本文对比研究了直接测定法、硝酸铝-亚硝酸钠-氢氧化钠比色法、三氯化铝比色法、差示分光光度法测定藤茶样品中总黄酮含量的准确性，并对方法的精密度、重复性、稳定性、加标回收率以及白砂糖、β-环糊精、羧甲基纤维素钠、果葡糖浆和焦糖色素对方法的干扰进行测定，最终用高效液相色谱法对结果进行验证。结果表明，以二氢杨梅素为对照品，在没有干扰物质的条件下，差示分光光度法最适合藤茶总黄酮的测定；该方法的精密度、重复性、稳定性和加标回收率的相对标准偏差（RSD）值分别为0.19%、2.26%、1.67%和100.97±1.63%。单独或者复合加入不同浓度的干扰物质，均对4种方法的检测结果造成明显的干扰。在复合干扰物存在时，直接测定法所测总黄酮含量最接近高效液相色谱法的测定结果。

The accuracy of direct measurement method, Al(NO3)3-NaNO2-NaOH colo rimetric method, AlCl3 colorimetric method and differential spectrophotometry for determining the total flavonoids in vine tea ( Ampelopsis grossedentata) were studied in this work. The accuracy, repeatability, stability, recovery rates of four methods were evaluated. The effects of white granulate sugar, β-CD, CMC-Na, fructose syrup and caramel on the interference for four methods were investigated. The results were validated by high performance liquid chromatography. The result showed that differential spectrophotometry was the most suitable method for determining the total flavonoids in vine tea with dihydromyricetin as the control and without interferent. The RSD for accuracy, repeatability, stability and recovery rates were 0.19%, 2.26%, 1.67% and 100.97%±1.63%, respectively. The addition of interferent with different concentration could cause the obvious interference to the detection results of the four methods. In the presence of complex interferent, the contents of total flavonoids determined by direct measurement method were most close to that determined by high performance liquid chromatography.

湖南省科技计划项目(2019NK3013-01；2018XF5050)；广东省重点研发计划项目（2019B020212003）；广州市科技项目(2016SK3038)；深圳市基础研究（自由探索）项目（JCYJ2018 0305163647462）