建立一种表面增强拉曼光谱（SERS）快速检测硫酸特步他林（TS）的方法。首先制备了表面增强活性基底，并通过电镜实验和紫外一可见吸收光谱对增强剂进行表征。通过实验证明制备出的银溶胶对硫酸特步他林拉曼信息增强效果最佳。因此, 筛选银溶胶作为表面增强剂。采用便携式拉曼光谱仪对水和尿液中的硫酸特步他林进行了检测，确定800 cm-1、1050 cm-1、1330 cm-1、1450 cm-1位移处的拉曼信号为硫酸特步他林的拉曼特征峰。通过进一步实验获得表面增强拉曼光谱信号强度与硫酸特步他林浓度在1~10 mg/L范围内呈现良好的线性关系，相关系数R2=0.97，检出限为0.5 mg/L，检测时间60 s。空白样品在三个水平的加标回收实验中，回收率分在为84.92%、91.63%、89.14%。与传统实验室大型仪器检测方法高效液相色谱法的对比实验结果具有较好的一致性。这项研究结果证明该方法具有快速、准确、无损、操作简便等优点，为“瘦肉精”监管打下了良好的基础。

A method for rapid detection of terbutaline sulfate by surface enhanced Raman spectroscopy (SERS) was developed. Firstly, the surface-enhanced active substrate was prepared, and the enhancer was characterized by electron microscopy and UV-Vis absorption spectroscopy. The experimental results showed that the prepared silver sol exhibited the greatest enhancing effect on the Raman signal information of terbutaline sulfate, thus, the silver sol was selected as the surface enhancer. A portable Raman spectrometer was used to detect terbutaline sulfate in water and urine. The Raman signals at 800 cm-1, 1050 cm-1, 1330 cm-1 and 1450 cm-1 were identified as the Raman characteristic peaks of terbutaline sulfate. A good linear relationship between the signal intensity of surface enhanced Raman spectrum and the concentration of terbutaline sulfate in the range of 1~10 mg/L was obtained through further experiments. The correlation coefficient R2 was 0.9718, with the detection limit as 0.5 mg/L and the detection time as 60 s. The recoveries of the blank samples were 84.9%, 91.6% and 89.1% in the three-levels standard spike-and-recovery experiments. These results were in good agreement with those of comparative experiments by high performance liquid chromatography-based traditional large-scale analysis instrument methods. The results of this research showed that the method is advantageous as it is fast, accurate, non-destructive and easy to operate, which laid a good foundation for the supervision of “lean meat powder”.

济南市引进创新团队资助项目（2020GXRC028）