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菌株Serratia marcescens SYBC08产过氧化氢酶液态发酵工艺的优化及酶性质研究
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国家863计划项目(2010AA101501)


Optimization of Fermentation Conditions for Catalase by Serratia marcescens SYBC08 and Characterization of Its Crude Enzyme
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    摘要:

    利用单因素筛选和正交试验对菌株Serratia marcescens SYBC08液态发酵产酶的培养基和条件进行了优化,其最优工艺为:柠檬酸25 g/L,玉米浆粉36 g/L,初始pH值为6.75,接种量为体积分数4%,装液量50 mL,转速250 r/min,35℃培养36 h产酶活力可达9 553 U/mL,是优化前的5.49倍。通过对硫酸铵沉淀得到的过氧化氢酶进行酶学性质研究,该酶在碱性条件(pH值为9.0)条件下,60℃下保温150 min酶活力几乎不变,65℃半衰期为150 min,其比商品化的牛肝过氧化氢酶具有更高的热稳定性。该酶最佳催化温度是20℃,在0℃依然展示了78%的活力。这些结果表明该酶具有良好的冷适应和热稳定性,在高温、碱性条件或极低温条件有应用潜力。

    Abstract:

    The nutrient and environmental conditions for catalase production by Serratia marcescens SYBC08 were optimized with single factor experiment and orthogonal design in this study.The optimum conditions were listed as follows: 25 g/L citric acid,36 g/L corn steep liquor powder,initial pH 6.75,liquid volume 50 mL/250 mL flask,4% inoculation,35 ℃,250 rpm,for 36 hours.With the optimum conditions,the catalase titer achieved at 9553 U/mL,which was 5.49-fold than that of the control.Properties of the catalase after purification by ammonium sulfate precipitation were studied.At 60 ℃ and pH 9.0,the enzyme was stable for 150 min;at 65 ℃ and pH 9,the half-life of the enzyme was approximately 150 min;its thermo stability was higher than that of commercial catalase from bovine.It was also active at 20 ℃ and had 78% of its activity at 0 ℃.These results suggest that the enzyme displays a property with cold adaption and thermo stability,and it has potential applications in high-temperature,alkaline and low-temperature conditions.

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曾化伟,张峰,蔡宇杰,廖祥儒,李娇阳,邢玉鹏,张大兵.菌株Serratia marcescens SYBC08产过氧化氢酶液态发酵工艺的优化及酶性质研究[J].食品与生物技术学报,2011,30(3):410-416.

ZENG Hua-wei, ZHANG Feng, CAI Yu-jie, LIAO Xiang-ru, LI Jiao-yang, XING Yu-peng, ZHANG Da-bin. Optimization of Fermentation Conditions for Catalase by Serratia marcescens SYBC08 and Characterization of Its Crude Enzyme[J]. Journal of Food Science and Biotechnology,2011,30(3):410-416.

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  • 在线发布日期: 2014-06-17
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