| 刘 辉,张 娟,张 燕,赵丹霞,吴胜泽.重组酶聚合酶快速扩增法测定瓶(桶)装水中铜绿假单胞菌[J].食品安全质量检测学报,2020,11(19):6915-6920 |
| 重组酶聚合酶快速扩增法测定瓶(桶)装水中铜绿假单胞菌 |
| Establishment of a recombinase polymerase amplification technology to detect Pseudomonas aeruginosa in bottled (or barreled) water |
| 投稿时间：2020-06-29 修订日期：2020-09-27 |
| DOI： |
| 中文关键词: 瓶(桶)装水 铜绿假单胞菌 重组酶聚合酶扩增法 |
| 英文关键词:bottled (or barreled) water Pseudomonas aeruginosa recombinase polymerase amplification |
| 基金项目:广州市科技计划项目(201804010244)、广州市科技计划项目(201904010102)、农业部农产品贮藏保鲜质量安全风险评估实验室(广州)暨广东省食品质量安全重点实验室开放课题(2019KF007) |
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| 中文摘要: |
| 目的 建立重组酶聚合酶扩增法(recombinase polymerase amplification, RPA)检测瓶(桶)装水中铜绿假单胞菌(Pseudomonas aeruginosa)的分析方法。方法 通过基因组DNA提取、RPA扩增反应方法对样品进行检测。结果 该方法能够在20 min内特异地检测出铜绿假单胞菌, 方法检出限为10 pg/μL铜绿假单胞菌基因组模板, 对人工污染的水样最低检出限为36 CFU/mL, 且重复性良好。结论 本研究建立的RPA检测方法能特异、准确、高效地检测出铜绿假单胞菌, 而且操作简单、耗时短, 为瓶(桶)装水中铜绿假单胞菌的快速诊断提供技术参考。 |
| 英文摘要: |
| Objective To establish a method for the determination of Pseudomonas aeruginosa in bottled (or barreled) water by recombinase polymerase amplification (RPA) technology. Methods The samples were detected by genomic DNA extraction and RPA amplification. Results The method could detect Pseudomonas aeruginosa within 20 min and the method detection limit was 10 pg/μL. The minimum detection limit of artificial pollution water sample was 36 CFU/mL. The method repeatability was good. Conclusion The established RPA detection method can detect Pseudomonas aeruginosa specifically, accurately and efficiently, and it is simple to operate and less time-consuming. It will provide a technical reference for the rapid diagnosis of Pseudomonas aeruginosa in bottled (or barreled) water. |
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