Uncovering the Rab5‐Independent Autophagic Trafficking of Influenza A Virus by Quantum‐Dot‐Based Single‐Virus Tracking |
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| Authors: | Qiu‐Mei Wu Shu‐Lin Liu Gang Chen Wei Zhang En‐Ze Sun Geng‐Fu Xiao Zhi‐Ling Zhang Dai‐Wen Pang |
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| Affiliation: | 1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies and Wuhan Institute of Biotechnology, Wuhan University, Wuhan, P. R. China;2. Key Laboratory of Oral Biomedicine (Ministry of Education) and Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan, P. R. China;3. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, P. R. China |
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| Abstract: | Autophagy is closely related to virus‐induced disease and a comprehensive understanding of the autophagy‐associated infection process of virus will be significant for developing more effective antiviral strategies. However, many critical issues and the underlying mechanism of autophagy in virus entry still need further investigation. Here, this study unveils the involvement of autophagy in influenza A virus entry. The quantum‐dot‐based single‐virus tracking technique assists in real‐time, prolonged, and multicolor visualization of the transport process of individual viruses and provides unambiguous dissection of the autophagic trafficking of viruses. These results reveal that roughly one‐fifth of viruses are ferried into cells for infection by autophagic machineries, while the remaining are not. A comprehensive overview of the endocytic‐ and autophagic‐trafficking process indicates two distinct trafficking pathway of viruses, either dependent on Rab5‐positive endosomes or autophagosomes, with striking similarities. Expressing dominant‐negative mutant of Rab5 suggests that the autophagic trafficking of viruses is independent on Rab5. The present study provides dynamic, precise, and mechanistic insights into the involvement of autophagy in virus entry, which contributes to a better understanding of the relationship between autophagy and virus entry. The quantum‐dot‐based single‐virus tracking is proven to hold promise for autophagy‐related fundamental research. |
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| Keywords: | autophagy influenza A virus quantum dots Rab5 single‐virus tracking |
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