Probing the heat‐induced structural changes in bovine serum albumin by fluorescence spectroscopy and molecular modelling |
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| Authors: | Florentina‐Mihaela Ursache Iuliana Aprodu Oana‐Viorela Nistor Mihaela Bratu Elisabeta Botez Nicoleta Stănciuc |
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| Affiliation: | Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, Galati, Romania |
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| Abstract: | The fluorescence spectroscopy and in silico methods were used to evaluate the structural changes in bovine serum albumin (BSA) over a temperature range of 25–70 °C for 15 min. Experimental results indicated that the polypeptide chain rearrangements at temperatures higher than 40 °C lead to a lower exposure of hydrophobic residues causing the decrease in fluorescence intensity. The nonlinearity of the phase diagram indicated a sequential denaturation process involving several molecular species. The molecular dynamics simulations at the single‐molecule level showed the high stability of the BSA structure, compensating for the entropic costs associated with the prevalent helical folding. |
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| Keywords: | Bovine serum albumin Structural changes Fluorescence spectroscopy Molecular modelling |
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