| L-半胱氨酸浓度对氧化还原电位和生物产氢过程的影响(英文) |
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| 引用本文： | 陈火晴,马晓轩,范代娣,骆艳娥,高鹏飞,杨婵媛.L-半胱氨酸浓度对氧化还原电位和生物产氢过程的影响(英文)[J].中国化学工程学报,2010,18(4):681-686. |
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| 作者姓名： | 陈火晴 马晓轩 范代娣 骆艳娥 高鹏飞 杨婵媛 |
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| 作者单位： | 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;2. Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical and Engineering, Northwest University, Xi'an 710069, China |
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| 基金项目： | the National High Technology Research and Development Program of China，the National Natural Science Foundation of China，the Specialized Research Fund for the Doctoral Program of Higher Education of China，Shaanxi Provincial Natural Science Foundation，Shaanxi Key Subject Program |
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| 摘 要： | The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,hydrogen production rate and hydrogen yield when its concentration was less than 500 mg·L-1,while it had negative effects when its concentration was higher than 500 mg·L-1.The hydrogen production was the highest 1.29 mol·mol-1(H2/glucose) when 300 mg·L-1L-cysteine was added into the culture,and the yield was 9.4% higher than that in the control.The oxidation-reduction potential(ORP) ,which was influenced by L-cysteine,also affected hydrogen production.The ORP values were in the range-300 mV to-150 mV when the L-cysteine concentration was higher than 500 mg·L-1.Although the ORP in this range was favorable for hydrogen production,it was not suitable for the biomass growth.Hence,less hydrogen was produced.When the L-cysteine concentration was lower than 500 mg·L-1,the ORP was more suitable for both biomass growth and hydrogen production.In addition,at least 91%glucose was consumed when L-cysteine was added to the culture media,compared to the 97.37% consumption without L-cysteine added.
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| 关 键 词： | anaerobic fermentation biohydrogen L-cysteine ORP |
| 收稿时间： | 2009-11-20 |
| 修稿时间： | 2009-11-20
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Influence of L-cysteine concentration on oxidation-reduction potential and biohydrogen production |
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| CHEN Huoqing,MA Xiaoxuan,FAN Daidi,LUO Yan''e,GAO Pengfei,YANG Chanyuan.Influence of L-cysteine concentration on oxidation-reduction potential and biohydrogen production[J].Chinese Journal of Chemical Engineering,2010,18(4):681-686. |
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| Authors: | CHEN Huoqing MA Xiaoxuan FAN Daidi LUO Yan'e GAO Pengfei YANG Chanyuan |
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| Affiliation: | 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;2. Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical and Engineering, Northwest University, Xi'an 710069, China |
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| Abstract: | The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation. The experimental results showed that L-cysteine could enhance the cell growth, hydrogen production rate and hydrogen yield when its concentration was less than 500 mg&;#8226;L&;#61485;1, while it had negative effects when its concentration was higher than 500 mg&;#8226;L&;#61485;1. The hydrogen production was the highest 1.29 mol&;#8226;mol&;#61485;1 (H2/glucose)] when 300 mg&;#8226;L&;#61485;1 L-cysteine was added into the culture, and the yield was 9.4% higher than that in the control. The oxidation-reduction potential (ORP), which was influenced by L-cysteine, also affected hydrogen production. The ORP values were in the range &;#61485;300 mV to &;#61485;150 mV when the L-cysteine con-centration was higher than 500 mg&;#8226;L&;#61485;1. Although the ORP in this range was favorable for hydrogen production, it was not suitable for the biomass growth. Hence, less hydrogen was produced. When the L-cysteine concentration was lower than 500 mg&;#8226;L&;#61485;1, the ORP was more suitable for both biomass growth and hydrogen production. In addition, at least 91% glucose was consumed when L-cysteine was added to the culture media, compared to the 97.37% consumption without L-cysteine added. |
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| Keywords: | anaerobic fermentation biohydrogen L-cysteine ORP |
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