| 化学修饰Novozym-435酶催化合成维生素E琥珀酸酯的研究 |
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| 引用本文： | 尹春华,张聪,高明.化学修饰Novozym-435酶催化合成维生素E琥珀酸酯的研究[J].中国化学工程学报,2011,19(1):135-139. |
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| 作者姓名： | 尹春华 张聪 高明 |
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| 作者单位： | Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China |
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| 基金项目： | Supported by the Fundamental Research Funds for the Central Universities and the State Key Development Program for Basic Research of China(2007CB714304) |
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| 摘 要： | Vitamin E succinate was synthesized in organic solvents using a modified Novozym-435 as catalyst.In order to improve the catalytic performance of Novozym-435,the enzyme was modified using acetic anhydride, propionic anhydride and succinic anhydride separately.We found that both the hydrolytic activity and the thermal stability of the modified Novozym-435 were enhanced compared with the unmodified enzyme.The modified Novozym-435 catalysts were used to synthesize the succinate derivative of vitamin E.Compared with the native Novozym-435,the catalytic activity of the modified novozym-435 in promoting the synthesis of vitamin E succinate was dramatically increased,with the novozym-435 modified with succinic anhydride(N435-S)as the most active catalyst.Conditions for the synthesis of vitamin E succinate were also optimized.A mixture of tert-butanol and DMSO(volume ratio of 2︰3)was the most suitable medium for the reaction,whereas the appropriate molar ratio of vitamin E to succinic anhydride and reaction temperature were 1︰5 and 40°C,respectively.Under these reaction conditions,the yield of vitamin E succinate reached 94.4%.N435-S could be reused for five batches.
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| 关 键 词： | lipase vitamin E succinate vitamin E modification |
| 收稿时间： | 2010-7-15 |
| 修稿时间： | 2010-11-15
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Enzyme-catalyzed synthesis of vitamin E succinate using a chemically modified Novozym-435 |
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| YIN Chunhua,ZHANG Cong,GAO Ming.Enzyme-catalyzed synthesis of vitamin E succinate using a chemically modified Novozym-435[J].Chinese Journal of Chemical Engineering,2011,19(1):135-139. |
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| Authors: | YIN Chunhua ZHANG Cong GAO Ming |
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| Affiliation: | Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China |
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| Abstract: | Vitamin E succinate was synthesized in organic solvents using a modified Novozym-435 as catalyst.In order to improve the catalytic performance of Novozym-435,the enzyme was modified using acetic anhydride, propionic anhydride and succinic anhydride separately.We found that both the hydrolytic activity and the thermal stability of the modified Novozym-435 were enhanced compared with the unmodified enzyme.The modified Novozym-435 catalysts were used to synthesize the succinate derivative of vitamin E.Compared... |
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| Keywords: | lipase vitamin E succinate vitamin E modification |
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