| DNA修饰纳米金溶胶的稳定性 |
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| 引用本文： | 杨薇,李(韦华),张金利. DNA修饰纳米金溶胶的稳定性[J]. 化工学报, 2006, 57(4): 970-974 |
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| 作者姓名： | 杨薇 李(韦华) 张金利 |
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| 作者单位： | 天津大学化工学院，天津 300072 |
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| 基金项目： | 中国科学院资助项目 , 教育部春晖计划项目 |
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| 摘 要： | 纳米金在分子生物学、生物传感器等领域具有广阔的应用前景.针对纳米金在电解质溶液中易形成不可逆聚集的问题，通过紫外光谱、TEM、Zeta电位测试等表征，研究了DNA分子修饰对纳米金溶胶稳定性的影响.结果表明，所制备的纳米金粒子的初始Zeta电位与粒径有关，平均粒径为13 nm的金粒子对应-44.4 mV的电位;当加入钠离子缓冲液后，纳米金粒子迅速聚集沉积.紫外光谱的动力学特性曲线表明，经过与巯基相连的DNA修饰后，纳米金粒子能够在钠离子缓冲液中稳定存在.
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| 关 键 词： | 纳米金 DNA修饰 胶体 粒子团聚 |
| 文章编号： | 0438-1157（2006）04-0970-05 |
| 收稿时间： | 2005-07-04 |
| 修稿时间： | 2005-07-042005-10-20 |
Stability of gold nanoparticles modified with DNA |
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| YANG Wei,LI Wei,ZHANG Jinli. Stability of gold nanoparticles modified with DNA[J]. Journal of Chemical Industry and Engineering(China), 2006, 57(4): 970-974 |
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| Authors: | YANG Wei LI Wei ZHANG Jinli |
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| Affiliation: | School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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| Abstract: | Gold nanoparticles show promising applications in the field of molecular biology and R&D of biological sensors. Aiming at overcoming the irreversible aggregation of nano-gold in electrolyte solutions, this work investigated the effect of DNA modification on nano-gold stability in the presence of sodium ions, through the characterization of UV, TEM and Zeta potential measurements. It was indicated that the Zeta potential of nano-gold depended on the diameter of particles. Nano-gold particles with diameter of 13 nm were stable at the Zeta potential of -44.4 mV, while irreversible aggregation of particles appeared quickly when a sodium buffer was added to the solution. UV spectra of kinetic properties for the DNA modified nano-gold particles reflected the high stability of the system with the addition of sodium ions. |
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| Keywords: | nano-gold DNA modification colloid particle aggregation |
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