| 不同硫源对小球诺卡氏菌R-9生长和脱硫活性的影响 |
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| 引用本文： | 罗明芳,邢建民,缑仲轩,李珊,刘国梁,谢渝春,刘会洲,陈家镛.不同硫源对小球诺卡氏菌R-9生长和脱硫活性的影响[J].化工学报,2003,54(10):1413-1417. |
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| 作者姓名： | 罗明芳 邢建民 缑仲轩 李珊 刘国梁 谢渝春 刘会洲 陈家镛 |
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| 作者单位： | 生化工程国家重点实验室、中国科学院过程工程研究所分离科学与工程青年实验室，北京100080 |
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| 基金项目： | 国家重点基础研究发展规划项目 (No G2 0 0 0 0 480 0 4)，中国石油化工(集团)公司资助项目(No X5 990 0 9)~~ |
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| 摘 要： | 用氯化钡沉淀结合傅里叶变换红外光谱法，证明小球诺卡氏菌（Nocardia globerula）R-9从二苯并噻吩中脱除的含硫产物以硫酸盐的形式存在于水相.比较了R-9分别以二苯并噻吩、硫酸钠和二甲基亚砜作为惟一硫源时的生长和脱硫活性.结果表明，二苯并噻吩、硫酸钠和二甲基亚砜均可以作为R-9的生长硫源，且高浓度（25 mmol•L-1）硫酸钠和二甲基亚砜对细胞的生长不产生抑制作用.2 mmol•L-1左右的硫酸钠或二甲基亚砜作为硫源时培养的细胞脱硫活性最高.提高硫源和碳源浓度可以提高R-9在发酵罐中的比生长速率和细胞浓度(OD600).
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| 关 键 词： | 比脱硫活性 二苯并噻吩 Nocardia globerula 生长 生物脱硫 |
| 文章编号： | 0438-1157(2003)10-1413-05 |
| 收稿时间： | 2002-5-22 |
| 修稿时间： | 2002年5月22日 |
EFFECTS OF DIFFERENT SULFUR SOURCES ON GROWTH AND DESULFURIZATION ACTIVITY OF Nocardia globerula R-9 |
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| LUO Mingfang,XING Jianmin,GOU Zhongxuan,LI Shan,LIU Guoliang,XIE Yuchun,LIU Huizhou and CHEN Jiayong.EFFECTS OF DIFFERENT SULFUR SOURCES ON GROWTH AND DESULFURIZATION ACTIVITY OF Nocardia globerula R-9[J].Journal of Chemical Industry and Engineering(China),2003,54(10):1413-1417. |
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| Authors: | LUO Mingfang XING Jianmin GOU Zhongxuan LI Shan LIU Guoliang XIE Yuchun LIU Huizhou and CHEN Jiayong |
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| Abstract: | Sulfate was identified as the final sulfur-containing product of DBT desulfurization by Nocardia globerula R-9 by means of precipitation with barium chloride and Fourier transform-infrared spectrometry. The growth and desulfurization activity of cells grown with DBT, sodium sulfate or dimethyl sulfoxide as the sulfur sources were investigated. The results showed that the said sulfur-containing compounds could be used as the sulfur sources of this strain. Furthermore, high concentration of sodium sulfate or dimethyl sulfoxide did not inhibit the growth of the strain. For the specific desulfurization activity, the optimal concentration of sulfur source was about 2 mmol•L-1. |
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| Keywords: | specific desulfurization activity dibenzothiophene Nocardia globerula growth biodesulfurization |
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