Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry |
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| Authors: | Firas Jumaah Margareta Sandahl Charlotta Turner |
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| Affiliation: | 1. , Department of Chemistry, Centre for Analysis and Synthesis, Lund University, Lund, Sweden;2. +46 46 222 8125+46 706 222 752 |
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| Abstract: | A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze‐dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively. This discrepancy between FAME and total lipids could be explained by the presence of wax esters, sterol esters, carotenoids and phospholipids, as determined by supercritical fluid chromatography. |
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| Keywords: | Bilberry Supercritical fluid extraction Supercritical fluid chromatography GC– MS Lipids Fatty acids |
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