| 基于非靶向代谢组学的红花大金不同生育期烟叶代谢图谱差异分析 |
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| 引用本文： | 向海英,刘欣,逄涛,曾婉俐,许力,高茜,邓乐乐,张建铎,李雪梅,李勇,孔维松. 基于非靶向代谢组学的红花大金不同生育期烟叶代谢图谱差异分析[J]. 中国烟草学报, 2022, 28(4): 77-84. DOI: 10.16472/j.chinatobacco.2021.236 |
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| 作者姓名： | 向海英 刘欣 逄涛 曾婉俐 许力 高茜 邓乐乐 张建铎 李雪梅 李勇 孔维松 |
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| 作者单位： | 1.云南中烟工业有限责任公司技术中心，云南省烟草化学重点实验室，云南昆明红锦路181号 650231 |
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| 基金项目： | 中国烟草总公司科技重大专项"烟草物质代谢相关核心基因的编辑文库构建及素材创制"110202001028（JY-11）云南中烟工业有限责任公司科技项目"两个烟草生物碱关联编辑素材的精细化筛选研究"2020JC03云南省烟草化学重点实验室开放课题"烟草高通量靶向代谢组学方法开发及在育种素材中的应用"2021539200340239 |
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| 摘 要： | ![]()
目的 为分析不同生育期烟草烟叶代谢图谱差异。 方法 以烟草品种红花大金元为研究对象，采集团棵期、现蕾期和成熟期烟叶样品，利用LC-QTOF/MS进行代谢指纹图谱检测，利用统计分析方法对差异代谢物进行筛选，基于Spearman相关分析计算代谢物之间的相关性系数，利用MetaboAnalyst软件进行代谢通路富集分析。 结果 （1）不同生育期烟草样品代谢图谱存在明显差异，共鉴定出245个差异代谢物，其中类黄酮、有机酸、氨基酸、核苷、生物碱、脂类等物质种类最多。（2）烟碱及其衍生物，如烟碱-1-氧代、麦斯明、假木贼碱、马钱子碱、新烟碱、2, 3'-二联吡啶，均随烟草生育期而逐渐累积，而与烟碱合成相关的前体物质，如精氨酸、亚精胺、腐胺、羟基丁酸等，均随着生育进程逐渐减少，且与烟碱含量显著负相关。（3）与次级代谢物合成相关氨基酸和有机酸等物质在团棵期或现蕾期含量较多，而在成熟期含量较低，与成熟期次级代谢物合成有关。（4）大部分类黄酮和酚酸组分在成熟期时含量较高，可以提高烟草抗病和抗逆能力。（5）相关性分析发现，烟碱含量与谷氨酸、泛酸、吡哆醛、蜜二糖、3-羟基邻氨基苯甲酸和顺式玉米素-o-葡萄糖苷等物质显著正相关，可能是调控烟叶中烟碱含量的重要标志物。 结论 基于代谢组学揭示了不同生育期烟草代谢物变化规律，为优质烟草育种及品质分析提供技术支撑。
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| 关 键 词： | 烟草 代谢组学 生育期 烟碱 差异代谢物 |
| 收稿时间： | 2021-11-22 |
Differential analysis of metabolic profiles of Honghua Dajinyuan tobacco leaves in different growth stage based on untargeted metabolomics |
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| Affiliation: | 1.Yunnan Key Laboratory of Tobacco Chemistry, R & D Center of China Tobacco Yunnan Industrial Co., Ltd., Kunming, 650231, China2.Yunnan Acedemy of Tobacco Agriculture Science, Kunming 650051, China3.Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | ![]()
Background The difference of metabolic profiling of tobacco leaves in different growth stages was investigated using untargeted metabolomics. Methods Tobacco leaves in cluster, budding and maturation stages were collected and analyzed by LC-QTOF/MS. Statistic analysis was conducted to test the significance of difference in the metabolites among different groups of tobacco leaves. Pathway analysis was performed on the MetaboAnalyst web-based software. The associations among metabolites were defined by Spearman correlation analysis. Results (1) There were significant differences in the metabolic profiles of tobacco samples in various growth stages. A total of 245 differential metabolites were identified, among which flavonoids, organic acids, amino acids, nucleosides, alkaloids and lipids were the dominant types. (2) Nicotine and its derivatives, such as 2, 3'-bipyridine, nornicotine, myosmine, anababine, nornicotine and anatabine and nicotine-1-oxo were gradually accumulated with maturation process; However, some synthesis precursor substances of nicotine such as arginine, and spermine, putrescine, 3-hydroxybutyric acid, exhibited the opposite trends. (3) The contents of amino acids and organic acids related to the synthesis of secondary metabolites were higher in cluster stage or budding stage than in the mature stage. (4) Most flavonoids and phenolic acids were higher in maturation stages, which could effectively improve resistance of tobacco plants to diseases and external stress. (5) Correlation analysis showed that the levels of glutamic acid, pantothenic acid, pyridoxal, meliciose, 3-hydroxyo-aminobenzoic acid and zeaxin-o-glucoside were significantly positively correlated with nicotine content, suggesting that these metabolites might be important markers for regulating nicotine synthesis in tobacco leaves. Conclusion This study reveals the change of tobacco metabolites in various growth stages, which provides technical support for tobacco breeding and quality analysis. |
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