| 22个烤烟品种苗期对增强UV-B辐射的耐性分析及鉴定指标筛选 |
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| 引用本文： | 顾浩,张锦韬,陈颐,李嘉亮,蔡虓,杨志新,王力,何承刚.22个烤烟品种苗期对增强UV-B辐射的耐性分析及鉴定指标筛选[J].中国烟草学报,2022,28(6):94-103. |
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| 作者姓名： | 顾浩 张锦韬 陈颐 李嘉亮 蔡虓 杨志新 王力 何承刚 |
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| 作者单位： | 1.云南农业大学烟草学院, 昆明市盘龙区金黑公路95号 650201 |
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| 基金项目： | 湖南中烟工业有限责任公司烟草农业示范与推广科技项目202143000934114云南省烟草公司科技计划重点项目2020530000241025 |
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| 摘 要： | 目的 对22个烤烟品种苗期耐UV-B辐射能力进行综合评价，并筛选耐性鉴定指标。 方法 测定22个烤烟品种苗期在自然UV-B辐射（对照）和4.60 kJ/m2·d增强UV-B辐射下的7项生长指标和9项生理指标，依据各单项指标对增强UV-B辐射的响应指数进行主成分分析，并通过隶属函数和系统聚类等分析方法，对22个烤烟品种苗期耐UV-B辐射能力进行综合评价，再结合逐步回归分析筛选烤烟苗期耐UV-B辐射鉴定指标。 结果 将16个单项指标进行主成分分析，得到5个相互独立的综合指标，通过隶属函数法计算得出各品种对增强UV-B辐射耐性评价值（D），利用聚类分析，筛选出7个对UV-B辐射耐性较强、4个对UV-B辐射耐性中等、7个对UV-B辐射耐性弱、4个对UV-B辐射较敏感的烤烟品种，采用逐步回归分析建立烤烟苗期对UV-B辐射耐性预测方程:D'=0.510+0.233SFW+0.135SOD+0.114UV-AS+0.113LDM－0.068W-SS+0.073RDM（R2=0.974，P＜0.05），其平均预测精度为95.24%。 结论 NC102、云烟87、云烟85、NC82、中烟201、NC297和中烟100对UV-B辐射耐性较强，中烟103、云烟201、云烟97和云烟203对UV-B辐射较敏感；筛选出幼苗鲜质量（SFW）、超氧化物歧化酶（SOD）活性、紫外吸收物质（UV-AS）含量、叶干质量（LDM）、水溶性糖（W-SS）含量和根干质量（RDM）作为烤烟苗期耐UV-B辐射鉴定指标。
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| 关 键 词： | UV-B辐射 烤烟品种 苗期 综合评价 |
| 收稿时间： | 2022-03-11 |
Analysis of the tolerance of 22 flue-cured tobacco varieties to enhanced UV-B radiation at seedling stage and screening of identification indices |
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| Affiliation: | 1.College of Tobacco Science, Yunnan Agricultural University, Kunming 650201, China2.China Tobacco Hunan Industrial Co., Ltd., Changsha 410007, China3.Yunnan Academy of Tobacco Agricultural Sciences, Kunming 650021, China |
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| Abstract: | Purpose This study aims to evaluate the tolerance of 22 flue-cured tobacco varieties to UV-B radiation at seedling stage and screen the identification indices. Methods Seven growth indices and nine physiological indices of 22 flue-cured tobacco cultivars were measured under natural UV-B radiation (control) and enhanced UV-B radiation (4.60 kJ/m2·d) at seedling stage. Principal component analysis was carried out according to the response indices of each single index to enhanced UV-B radiation, the UV-B radiation tolerance of 22 flue-cured tobacco varieties at seedling stage was comprehensively evaluated by means of membership letter and systematic cluster analysis, and the identification indices of UV-B radiation tolerance of flue-cured tobacco at seedling stage were selected by stepwise regression analysis. Results Through principal component analysis of 16 single indices, five independent comprehensive indices were obtained. The evaluation value (D) of enhanced UV-B radiation tolerance of each variety was calculated by membership function method. Using cluster analysis, seven flue-cured tobacco varieties with strong UV-B radiation tolerance, four with medium UV-B radiation tolerance, seven with weak UV-B radiation tolerance and four with relatively weak UV-B radiation tolerance were selected, and the prediction equation of flue-cured tobacco's tolerance to UV-B radiation at seedling was established by stepwise regression analysis: D'=0.510+0.233SFW+0.135SOD+0.114UV-AS+0.113LDM-0.068W-SS+0.073RDM(R2=0.974, P < 0.05). The average predicted accuracy was 95.24%. Conclusion NC102, Yunyan 87, Yunyan 85, NC82, Zhongyan 201, NC297 and Zhongyan 100 high higher tolerance to UV-B radiation, while Zhongyan 103, Yunyan 201, Yunyan 97 and Yunyan 203 are more sensitive to UV-B radiation. Seedling fresh weight (SFW), sup eroxide dismutase (SOD) activity, UV absorbing substance (UV-AS), leaf dry mass (LDM), water-soluble sugar (W-SS) content and root dry mass (RDM) were selected as the evaluation indices of UV-B radiation resistance of flue-cured tobacco at seedlings. |
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