Characterization of Crude and Partially Purified Lipase from Geotrichum candidum Obtained with Different Nitrogen Sources |
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| Authors: | Rafael Resende Maldonado Eduardo Luiz Pozza Elizama Aguiar-Oliveira Fátima Aparecida Almeida Costa Francisco Maugeri Filho Maria Isabel Rodrigues |
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| Affiliation: | 1. +55 19 3521‐9900;2. , Food Department, Technical College of Campinas (COTUCA), University of Campinas (UNICAMP), Campinas, S?o Paulo, Brazil;3. +55 19 3521‐4052;4. , Laboratory of Bioprocess Engineering (LEB), Department of Food Engineering (DEA), Faculty of Food Engineering, University of Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, Campinas, S?o Paulo, Brazil;5. +55 77 34292709;6. , Bioscience Department, Multidisciplinary Institute on Health, Federal University of Bahia, Vitória da Conquista, Bahia, Brazil |
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| Abstract: | Lipases from Geotrichum candidum were produced in two different medium: A = 12 % (w/v) clarified corn steep liquor (CCSL) + 0.6 % (w/v) soybean oil (SO) and B = 3.5 % (w/v) yeast hydrolysate (YH) + 0.7 % (w/v) SO. Lipases were partially purified from both media by hydrophobic interaction chromatography using 3.0 mol L?1 of NaCl as mobile phase, and they were characterized in the crude and partially purified forms. The recovery of lipase activity from CCSL and YH via HIC were 96 and 94.3 %, and the purification factors were 44.3 and 86.7‐fold, respectively. All evaluated lipases had similar optimum pH (7.0–7.7), but, for the CCSL crude lipase, optimum temperature (47 °C) was 10 °C higher than others lipases evaluated. CCSL crude lipase possessed a higher thermo stability than YH crude lipase, e.g., at 37 °C (pH 7.0) the half‐life of CCSL crude lipase was 19.25 h and at pH 8.0 (30 °C) the half‐life was 48 h, which are five and ten times higher than with YH crude lipase, respectively. On the other hand, the YH crude lipase possessed a higher catalytic constant (kcat = 2.3 min?1) but with almost the same catalytic efficiency (Km/kcat = 32.12 mg mL min?1) in relation to CCSL crude lipase. The lipases differ in biocatalytic properties between substrates, suggesting that the two lipases can be employed for different applications. |
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| Keywords: | Central composite design Clarified corn steep liquor Fermentation Geotrichum candidum Hydrophobic interaction chromatography Lipase Thermo stability Yeast hydrolysate |
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