Combined Urea Complexation and Argentated Silica Gel Column Chromatography for Concentration and Separation of PUFAs from Tuna Oil: Based on Improved DPA Level |
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| Authors: | Hongyan Mu Jun Jin Dan Xie Xiaoqiang Zou Xiaosan Wang Xingguo Wang Qingzhe Jin |
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| Affiliation: | 1. , State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, People's Republic of China;2. , College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, People's Republic of China;3. Zhong Hai Ocean (wuxi) Marine Equipment Engineering Co., Ltd., Jiangnan University National University Science Park, Wuxi, Jiangsu, People's Republic of China;4. 86+ 510 8587 6799 |
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| Abstract: | Eicosapentaenoic acid (EPA, 20:5n‐3), docosapentaenoic acid (DPA) isomers (22:5n‐6 and 22:5n‐3) and docosahexaenoic acid (DHA, 22:6n‐3) derived from tuna oil were concentrated by three stages of urea fractionation at various crystallization temperatures and different fatty acid/urea ratios. Thereafter, polyunsaturated fatty acids concentrate containing comparatively enriched DPA levels was purified by argentated silica gel column chromatography. A product containing 22.2 ± 0.6 % EPA, 4.6 ± 0.0 % DPAn‐6, 5.9 ± 0.1 % DPAn‐3 and 42.3 ± 1.2 % DHA was obtained at 1:1.6 fatty acid/urea ratio (w/w) by crystallization at ?8 °C for 16 h, ?20 °C for 8 h, and ?8 °C for 16 h. A DPA isomer concentrate containing 26.1 ± 0.5 % DPAn‐6 and 22.3 ± 0.4 % DPAn‐3 was achieved by argentated silica gel chromatography in the 6 % acetone/n‐hexane solvent fraction (v/v), and the recovery of both fatty acids was 66.1 ± 3.2 and 70.7 ± 2.2 %, respectively. Furthermore, 91.9 ± 2.5 % EPA and 99.5 ± 2.1 % DHA with recoveries of 47.8 ± 2.0 and 56.7 ± 3.3 %, respectively, were obtained in various fractions. |
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| Keywords: | Tuna oil Urea inclusion Eicosapentaenoic acid Docosapentaenoic acid Docosahexaenoic acid Argentated silica gel column chromatography |
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