| 高效液相色谱法检测牙鲆体内的生物胺 |
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| 引用本文： | 赵中辉,林洪,徐杰. 高效液相色谱法检测牙鲆体内的生物胺[J]. 现代食品科技, 2011, 27(2): 228-231 |
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| 作者姓名： | 赵中辉 林洪 徐杰 |
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| 作者单位： | 中国海洋大学食品科学与工程学院,山东,青岛,266003 |
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| 摘 要： | 采用高效液相色谱(HPLC)法测定了牙鲆体内的多种生物胺.以邻苯二甲醛(OPA)为柱后荧光衍生剂,通过Capcell Pak MG C18色谱柱的梯度洗脱,将酪胺、腐胺、尸胺、组胺、胍丁胺、亚精胺和精胺等7种生物胺进行良好分离.测试结果表明,7种生物胺在0.05～30mg/L的范围内线性良好,相关系数都在0.999以上...
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| 关 键 词： | 高效液相色谱 柱后衍生 生物胺 牙鲆 |
Determination of Biogenic Amines in Paralichthys olivaceus by High Performance Liquid Chromatography |
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| ZHAO Zhong-hui,LIN Hong,XU Jie. Determination of Biogenic Amines in Paralichthys olivaceus by High Performance Liquid Chromatography[J]. Modern Food Science & Technology, 2011, 27(2): 228-231 |
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| Authors: | ZHAO Zhong-hui LIN Hong XU Jie |
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| Affiliation: | (College of Food Science and Engineering,Ocean University of China,Qingdao,266003) |
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| Abstract: | The method for determination of biogenic amines in Paralichthys olivaceus by reversed-phase high performance liquid chromatography and post-column derivatization with o-Phthalaldehyde(OPA) was developed in this paper.Seven amines(tyramine,putrescine, cadaverine,histamine,agmatine,spermidine and spermine) were well separated with this method.And the seven amines had a good linearity in the range from 0.05 to 30 mg/L,with the correlation coefficients being above 0.999(n=6).The average recoveries and the relative standard deviations of the seven amines in spiked sample were of 88.8%-106.9%and 3.0%～9.3%,respectively.The detection limits of all amines were 1.5μg/ L.In conclusion,this method was rapid and sensitive,with high recovery and fine reproducibility,which can be used to detect biogenic amines in Paralichthys olivaceus. |
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| Keywords: | high performance liquid chromatography(HPLC) post-column derivatization biogenic amine Paralichthys olivaceus |
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