| 脂肪酶位置选择性的影响因素 |
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| 引用本文： | 康佳惠子,王秀秀,贾敏,徐同成,宗爱珍,杜方岭,黄凤洪,尤艳莉.脂肪酶位置选择性的影响因素[J].中国油脂,2023,48(7):115-119. |
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| 作者姓名： | 康佳惠子 王秀秀 贾敏 徐同成 宗爱珍 杜方岭 黄凤洪 尤艳莉 |
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| 作者单位： | 1.烟台大学 生命科学学院，山东 烟台 264005； 2.山东省农业科学院 农产品加工与营养研究所/山东省特殊医学用途配方
食品工程技术研究中心/山东省农产品精深加工技术重点实验室/农业农村部新食品资源加工重点实验室，济南 250100 |
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| 基金项目： | 泰山学者工程（黄凤洪）；山东省重点研发计划（医用食品）（2019YYSP027） |
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| 摘 要： | 为开发高效、绿色、低成本的脂肪酶应用于结构脂质的制备，从内源性(脂肪酶的来源、基因序列、空间位阻)和外源性(底物空间结构、反应介质、固定化条件及反应pH)两方面讨论了脂肪酶位置选择性的影响因素。脂肪酶催化特异性由内源性因素和外源性因素共同决定。改变底物空间结构、反应介质、固定化条件和反应pH,常被用作提高脂肪酶催化性能的处理方式。只有充分考虑内外两方面因素的影响，才能充分提高脂肪酶的位置选择性和催化效率。该综述有望为高选择性脂肪酶的开发开辟新的研究方向，为后续结构脂质的高效合成提供启发性的研究思路。
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| 关 键 词： | 脂肪酶 位置选择性 结构脂质 |
Factors affecting the regioselectivity of lipase |
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| KANG Jiahuizi,WANG Xiuxiu,JIA Min,XU Tongcheng,ZONG Aizhen,DU Fangling,HUANG Fenghong,YOU Yanli.Factors affecting the regioselectivity of lipase[J].China Oils and Fats,2023,48(7):115-119. |
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| Authors: | KANG Jiahuizi WANG Xiuxiu JIA Min XU Tongcheng ZONG Aizhen DU Fangling HUANG Fenghong YOU Yanli |
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| Affiliation: | 1.School of Life Sciences, Yantai University,Yantai 264005, Shandong, China; 2.Institute of
Agro-Food Science and Technology/Shandong Engineering Research Center for Formulated Foods for
Special Medical Purposes/Key Laboratory of Advanced and Deep Processing Technology of Agricultural
Products of Shandong Province/Key Laboratory of New Food Resource Processing of Ministry of
Agriculture and Rural Affairs, Shandong Academy of Agricultural Sciences, Jinan 250100, China |
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| Abstract: | To develop efficient, green and low-cost lipases for the preparation of structured lipid, the factors affecting lipase regioselectivity were discussed in terms of both endogenous (source of lipase, gene sequence, spatial resistance) and exogenous (substrate spatial structure, reaction medium, immobilization conditions and reaction pH). The lipases catalytic specificity were determined by the endogenous and exogenous factors, and changing the substrate spatial structure, reaction medium, immobilization conditions and reaction pH, were often used as treatment ways to improve the catalytic performance of the lipase. Only by fully considering the effects of both internal and external factors, the regioselectivity and catalytic efficiency could be improved. The summarize is expected to develop new research directions for the development of highly selective lipase and provide enlightening research ideas for the subsequent efficient synthesis of structured lipid. |
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| Keywords: | lipase regioselectivity structured lipid |
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