| 菌源处理方式对发酵产氢微生物菌群及产氢性能的影响 |
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| 引用本文： | 刘义,樊绍群,徐耀伟,樊耀亭,侯红卫.菌源处理方式对发酵产氢微生物菌群及产氢性能的影响[J].化工学报,2010,61(6):1545-1549. |
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| 作者姓名： | 刘义 樊绍群 徐耀伟 樊耀亭 侯红卫 |
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| 作者单位： | 郑州大学化学系;中国电信股份有限公司北京研究院 |
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| 基金项目： | 国家重点基础研究发展计划项目，国家自然科学基金，高校博士点专项科研基金，教育部郑州大学"211"工程项目 |
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| 摘 要： | 菌源预处理方式是影响和制约发酵生物制氢效率的关键因素之一。本文以牛粪堆肥为产氢菌源，在20 L连续流搅拌槽式反应器(CSTR)反应器中，考察了红外烘干、浸泡、红外烘干结合曝气、红外烘干结合加糖曝气四种菌源预处理方式对发酵产氢微生物菌落及产氢性能的影响。结果表明，在T为36±1℃、pH为5.0±0.1、C：N：P=500：5：1、底物浓度为10 g/L、HRT为11 h的条件下，牛粪堆肥经红外烘干处理4 h后进一步加糖曝气3天，获得最大产气速率和最大氢浓度分别为0.5089 L H2·L·h) -1和55.5%。通过PCR-DGGE指纹图谱分析对不同菌源预处理条件下发酵产氢系统中微生物群落结构的变化信息进行了比较分析。产氢验证实验表明：在最佳产氢条件下，以红外烘干结合加糖曝气预处理的牛粪堆肥为产氢菌源，以蔗糖为底物，得到的最大氢产量、氢浓度、产氢速率分别为：387.6 ml H2/g-sucrose、55.5%和0.5089 L H2·L·h) -1.液相末端发酵产物中丁酸和乙酸占VFAs总量的75%以上。
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| 关 键 词： | 生物制氢 菌源处理方式 凝胶电泳分析 发酵 |
| 收稿时间： | 2009-10-14 |
| 修稿时间： | 2009-12-1
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Effect of treatment methods for natural bacteria source on microbial community and bio-hydrogen-producing potential |
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| LIU Yi,FAN Shaoqun,XU Yaowei,FAN Yaoting,HOU Hongwei.Effect of treatment methods for natural bacteria source on microbial community and bio-hydrogen-producing potential[J].Journal of Chemical Industry and Engineering(China),2010,61(6):1545-1549. |
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| Authors: | LIU Yi FAN Shaoqun XU Yaowei FAN Yaoting HOU Hongwei |
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| Abstract: | The pretreatment of bacteria source is one of the key factors affecting the efficiency of bio-hydrogen production.Prior to inoculation, cow dung compost as the natural microbial consortium is treated by following methods: (1)forced aeration combined adding sugar after infrared drying; (2)infrared drying; (3)infrared drying combined forced aeration; and (4)water immersion.The effects of the microbial community on bio-hydrogen production were investigated in a 20 L continuous stirred tank reactor (CSTR).The results showed that the maximum hydrogen production ability, hydrogen content and the rate of hydrogen production was 387.6 ml H2·g-sucrose) -1, 55.5% and 0.5089 L H2·L·h) -1 at fixed sucrose concentration of 10 g·L-1, operating pH of 5.0±0.1 and the pretreated bacteria source by method (1), respectively.The contents of acetic acid and butyric acid were more than 75% of VFAs in the terminal liquid phase by-product.Furthermore, the image analysis by PCR-DGGE technology showed that there was district difference in the number of electrophoresis band and the strength.The sample, of which the bacteria source was treated by method (1), exhibited two maximum illumination bands, which implies that there exist two kinds of dominant microbial community. |
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| Keywords: | bio-hydrogen production treatment method of bacteria source PCR-DGGE fermentation |
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