Self‐Assembled Injectable Nanocomposite Hydrogels Stabilized by Bisphosphonate‐Magnesium (Mg2+) Coordination Regulates the Differentiation of Encapsulated Stem Cells via Dual Crosslinking |
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| Authors: | Kunyu Zhang Qian Feng Jianbin Xu Xiayi Xu Feng Tian Kelvin W K Yeung Liming Bian |
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| Affiliation: | 1. Division of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong, P. R. China;2. Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong, P. R. China;3. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, P. R. China;4. Department of Orthopaedics and Traumatology, The University of Hong Hong, Hong Kong, P. R. China;5. Shun Hing Institute of Advanced Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong, P. R. China;6. Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, P. R. China;7. China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, P. R. China;8. Center for Novel Biomaterials, The Chinese University of Hong Kong, Hong Kong, P. R. China |
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| Abstract: | Nanocomposite hydrogels consist of a polymer matrix embedded with nanoparticles (NPs), which provide the hydrogels with unique bioactivities and mechanical properties. Incorporation of NPs via in situ precipitation in the polymer matrix further enhances these desirable hydrogel properties. However, the noncytocompatible pH, osmolality, and lengthy duration typically required for such in situ precipitation strategies preclude cell encapsulation in the resultant hydrogels. Bisphosphonate (BP) exhibits a variety of specific bioactivities and excellent binding affinity to multivalent cations such as magnesium ions (Mg2+). Here, the preparation of nanocomposite hydrogels via self‐assembly driven by bisphosphonate‐Mg2+ coordination is described. Upon mixing solutions of polymer bearing BPs, BP monomer (Ac‐BP), and Mg2+, this effective and dynamic coordination leads to the rapid self‐assembly of Ac‐BP‐Mg NPs which function as multivalent crosslinkers stabilize the resultant hydrogel structure at physiological pH. The obtained nanocomposite hydrogels are self‐healing and exhibit improved mechanical properties compared to hydrogels prepared by blending prefabricated NPs. Importantly, the hydrogels in this study allow the encapsulation of cells and subsequent injection without compromising the viability of seeded cells. Furthermore, the acrylate groups on the surface of Ac‐BP‐Mg NPs enable facile temporal control over the stiffness and crosslinking density of hydrogels via UV‐induced secondary crosslinking, and it is found that the delayed introduction of this secondary crosslinking enhances cell spreading and osteogenesis. |
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| Keywords: | dynamic coordination nanocomposite hydrogels self‐assembly stem cell differentiation |
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