| 液相色谱串联质谱法测定加工食品中丙烯酰胺的含量 |
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| 引用本文： | 刘刚,王毅,王鑫,雷激,何楠.液相色谱串联质谱法测定加工食品中丙烯酰胺的含量[J].西华大学学报(自然科学版),2021,40(2):97-102. |
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| 作者姓名： | 刘刚 王毅 王鑫 雷激 何楠 |
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| 作者单位： | 1.四川省食品药品检验检测院，四川 成都 611731 |
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| 摘 要： | 建立了高效液相色谱串联质谱法测定加工食品中丙烯酰胺的方案；对多种类型的加工食品基质采用提取净化措施，利用高效液相色谱质谱对目标物质进行分离定性，结合同位素内标定量法对样本中丙烯酰胺进行定量分析。通过优化色谱条件和质谱参数，丙烯酰胺上机质量浓度在3~2 000 ng/mL具有良好的线性相关性，相关系数R≥0.999，检出限为3 μg/kg，定量限为10 μg/kg。对不同基质样本进行考察，方法表现出良好的稳定性和准确性，具有良好的回收率，适用于加工类食品中丙烯酰胺的定性定量分析。
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| 关 键 词： | 丙烯酰胺 同位素内标法 液相色谱质谱 加工食品 |
| 收稿时间： | 2020-06-18 |
Determination of Acrylamide in Processed Foods by Liquid Chromatography Tandem Mass Spectrometry |
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| LIU Gang,WANG Yi,WANG Xin,LEI Ji,HE Nan.Determination of Acrylamide in Processed Foods by Liquid Chromatography Tandem Mass Spectrometry[J].Journal of Xihua University:Natural Science Edition,2021,40(2):97-102. |
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| Authors: | LIU Gang WANG Yi WANG Xin LEI Ji HE Nan |
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| Affiliation: | 1.Sichuan Institute for Food and Drug Control, Chengdu 611731 China |
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| Abstract: | To establish a method for the determination of acrylamide in processed foods by using high performance liquid chromatography tandem mass spectrometry, series of processed food substrates were extracted and purified, and then the target substance was separated and qualitative analyzed with LCMSMS, and the isotopic internal standard quantitative method was also used to determine the qualitative of acrylamide in processed foods. By optimizing the chromatographic condition and mass spectrum parameters, the acrylamide standard solution from 3 ng/mL to 2 000 ng/mL had a good linear relationship (LR)with LCMSMS, the LR≥0.999, and the detection limit was 3 μg/kg, and the quantitative limit was 10 μg/kg and the detection method also had good recovery. The method had good stability and accuracy and good recovery rate. It was suitable for qualitative and quantitative analysis of acrylamide in processed food. |
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