On‐the‐Spot Immobilization of Quantum Dots,Graphene Oxide,and Proteins via Hydrophobins |
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| Authors: | Alfredo M Gravagnuolo Eden Morales‐Narváez Charlene Regina Santos Matos Sara Longobardi Paola Giardina Arben Merkoçi |
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| Affiliation: | 1. ICN2–Catalan Institute of Nanoscience and Nanotechnology, Barcelona, Spain;2. Department of Chemical Sciences, University of Naples “Federico II,”, Naples, Italy;3. ICREA–Catalan Institution for Research and Advanced Studies, Barcelona, Spain |
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| Abstract: | Class I hydrophobin Vmh2, a peculiar surface active and versatile fungal protein, is known to self‐assemble into chemically stable amphiphilic films, to be able to change wettability of surfaces, and to strongly adsorb other proteins. Herein, a fast, highly homogeneous and efficient glass functionalization by spontaneous self‐assembling of Vmh2 at liquid–solid interfaces is achieved (in 2 min). The Vmh2‐coated glass slides are proven to immobilize not only proteins but also nanomaterials such as graphene oxide (GO) and quantum dots (QDs). As models, bovine serum albumin labeled with Alexa 555 fluorophore, anti‐immunoglobulin G antibodies, and cadmium telluride QDs are patterned in a microarray fashion in order to demonstrate functionality, reproducibility, and versatility of the proposed substrate. Additionally, a GO layer is effectively and homogeneously self‐assembled onto the studied functionalized surface. This approach offers a quick and simple alternative to immobilize nanomaterials and proteins, which is appealing for new bioanalytical and nanobioenabled applications. |
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| Keywords: | graphene oxide quantum dots self‐assembling surface functionalization Vmh2 hydrophobin |
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