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| Survivin、Caspase-3、cyclinD1在骨肉瘤中的表达及临床意义 | |
| 引用本文： | 吴庆,吴凯,冯琼,付晓玲. Survivin、Caspase-3、cyclinD1在骨肉瘤中的表达及临床意义[J]. 矿产勘查, 2010, 0(1): 43-46,49 |
| 作者姓名： | 吴庆 吴凯 冯琼 付晓玲 |
| 作者单位： | [1]南昌大学第二附属医院骨科,南昌330006 [2]南昌大学医学院护理部,南昌330006 |
| 摘 要： | 目的研究Survivin、Caspase-3、cyclinD1在骨肉瘤中的表达及其临床意义。方法应用免疫组化S-P法检测36例骨肉瘤中Survivin、Caspase-3、cyclinD1的表达水平以及Survivin与Caspase-3、cyclinD1两者之间的相关性，并对所有病例进行随访。10例骨软骨瘤为对照组。结果①Survivin在骨肉瘤中的阳性率为63．7％（23／36），明显高于对照组骨软骨瘤10．0％（1／10）的阳性率，两者相比差异有统计学意义（P〈0．01）；Caspase-3在骨肉瘤中的阳性表达率为52．8％（19／36），而在骨软骨瘤中为90．0％（9／10），两者相比差异有统计学意义（P〈0．01）；cyclinD1蛋白在骨肉瘤中的阳性率为74．4％（29／36），高于在骨软骨瘤中的40．0％（4／10），两者相比差异有统计学意义（P〈0．05）；前2者表达部位在细胞浆，cyclinD1表达于细胞核。②Survivin和Caspase-3表达强度之间呈显著负相关（r=-0．46，P〈0．01）。⑧Survivin与cyclinD1表达呈正相关（r=-0．641，P〈0．01）。结论①Survivin在骨肉瘤组织中过表达与骨肉瘤的发生有关。②Caspase-3在骨肉瘤组织中低表达，Survivin可能通过抑制Caspase-3活性从而发挥其抑制骨肉瘤细胞的凋亡。③Survivin和cyclinD1在骨肉瘤的发生发展中可能起协同作用。 |
| 关 键 词： | 骨肉瘤 Survivin Caspase-3 cyclinD1 免疫组织化学 |
Clinical Significance and Expression Survivin,Caspase-3 and cyclinD1 in Osteosarcoma |
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| WU Qing,WU Kai,FENG Qiong,FU Xiao-ling. Clinical Significance and Expression Survivin,Caspase-3 and cyclinD1 in Osteosarcoma[J]. Mineral Exploration, 2010, 0(1): 43-46,49 | |
| Authors: | WU Qing WU Kai FENG Qiong FU Xiao-ling |
| Affiliation: | (a. Dpeartment of Orthopaedics, the Second Affiliated Hospital ;b. Department of Nursing ,Medical College ,Nanchang University ,Nanchang 330006 ,China) |
| Abstract: | Objective To investigate the expression of Survivin,Caspase-3 and cyclinD1 in osteosarcoma and its clinical signficance. Methods Using immunohistoehemistry （S-P method） to detect the expressions of Survivin,Caspase-3 and cyclinD1 in 36 osteosarcoma tissues and 10 ostanchondroma tissues. Results （1）The expression rate of Survivin was significantly higher in osteosarcoma （63.7% ,23/36） than that in osteochondroma（10.0%, 1/10）（P〈0.01）. The expression of Caspase-3 was detected in a greater proportion of osteochondroma（90.0% ,9/10） than that in osteosareoma（52.8%, 19/36）（P〈0.01）. The positive expression rate of cyclinD1 was higher in osteosareoma（74.4% ,29/36） than that in osteochondroma（40.0% ,4/10）. The former two are expressed in the cytoplasm,cyclinD1 is expressed in the nucleus. （2）There was a negative correlation was observed between the Survivin and Caspase-3 expressions in osteosarcoma（r=-0.46,P〈0.01）. （3） There was a significantly positive correlation between Survivin and cyclinD 1 （r = 0. 641, P〈 0. 01）. Conclusion （1） Survivin is over expressed in osteosmcoma. （2） Survivin exert probably the act of restraining oseteisarcoma cell apoptosis by the pathway of Caspase-3. （3）Survivin and cyclinD1 light play synergetie roles in the process of osteosarcoma. |
| Keywords: | osteosarcoma survivin caspase-3 cyclinD1 immunohistochemistry |
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