| SRT对富集高聚PHA能力嗜盐MMC的影响 |
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| 引用本文： | 崔有为,林小媛,冀思远,施云鹏.SRT对富集高聚PHA能力嗜盐MMC的影响[J].化工学报,2016,67(6):2575-2582. |
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| 作者姓名： | 崔有为 林小媛 冀思远 施云鹏 |
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| 作者单位： | 北京工业大学环境与能源工程学院, 北京 100124 |
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| 基金项目： | 国家自然科学基金项目(51478011,51178004);北京市自然科学基金项目(8132013);北京市属高等学校高层次人才引进与培养计划项目。 |
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| 摘 要： | 调控菌群富集过程的工艺参数,富集具有高PHA积累能力的混合菌(MMC)是发酵生产PHA的第一步也是最重要的一步。采用批式培养条件,集中研究了污泥停留时间(SRT)对PHA积累菌群的富集作用。研究结果表明SRT影响富集MMC的底物降解速率(qS),微生物积累PHA的速率(qP)和PHA积累能力。长SRT(SRT4d)导致了较低的qS,qP和PHA细胞含量;过短的SRT(SRT1d)则降低了MMC的PHA转化率(YPHA/S)和PHA积累能力,刺激了非PHA积累菌群的快速增殖。研究确定的最佳SRT为2d,在此条件下富集的MMC最大PHA积累量可达细胞干重的56%。研究证明了SRT在选择嗜盐MMC中的重要作用,为快速富集PHA积累能力MMC奠定基础。
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| 关 键 词： | SRT PHA积累菌 聚合物 发酵 生物工程 |
| 收稿时间： | 2015-11-02 |
| 修稿时间： | 2016-02-29 |
Influence of SRT on enriching halophilic MMC with capacity of PHA storage |
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| CUI Youwei,LIN Xiaoyuan,JI Siyuan,SHI Yunpeng.Influence of SRT on enriching halophilic MMC with capacity of PHA storage[J].Journal of Chemical Industry and Engineering(China),2016,67(6):2575-2582. |
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| Authors: | CUI Youwei LIN Xiaoyuan JI Siyuan SHI Yunpeng |
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| Affiliation: | College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China |
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| Abstract: | Enriching mixed microbial culture (MMC) with capacity of PHA storage via controlling the enrichment process parameters is the first and the most important step for the fermentation production of PHA. This paper elucidated the role of sludge retention time (SRT) in the enrichment process under batch culture conditions. The results showed that SRT influenced the specific substrate degradation rate (qS), specific PHA accumulation rate (qP) and the PHA storage capacity of enriched MMC. The long SRT (SRT 4 d) led to the decreased qS, qP and PHA accumulation ability. The short SRT reduced the PHA conversion rate (YPHA/S) and PHA accumulation ability of MMC. The optimal SRT was determined as 2 d for enriched MMC in this study, which presented 56% cell dry weight PHA content. The study proved that the SRT played the important role in enriching halophilic MMC, which laid the foundation on enriching PHA-storage MMC rapidly. |
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| Keywords: | SRT PHA storage bacteria polymers fermentation biological engineering |
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