| 双水相分配结合温度诱导相分离从酵母中提取谷胱甘肽 |
| |
| 引用本文： | 梅乐和,林东强.双水相分配结合温度诱导相分离从酵母中提取谷胱甘肽[J].化工学报,1998,49(4):470-475. |
| |
| 作者姓名： | 梅乐和 林东强 |
| |
| 作者单位： | 浙江大学生物化工系!杭州310027,浙江大学生物化工系!杭州310027,浙江大学生物化工系!杭州310027 |
| |
| 基金项目： | 国家自然科学基金!29731680,29706009 |
| |
| 摘 要： | 对用双水相分配技术结合温度诱导相分离技术从酵母中提取谷胱甘肽（GSH）进行了研究．考察了GSH在环氧乙烷──环氧丙烷无规共聚物（EOPO）／羟丙基淀粉（PES）系统中的分配行为，包括两次分离过程（双水相分配及温度诱导过程)中的不同系统组成、pH等对GSH分配的影响，确定了较佳的双水相系统──EOPO400013％，PES10010％，pH=10.5．在此基础上进行了从酵母细胞中提取GSH的工艺研究，设计了合理的分离流程，研究结果表明GSH的总萃取率可达80％以上．
|
| 关 键 词： | 温度诱导相 分离 谷胱甘肽 提取 酵母细胞 |
SEPARATION OF GLUTATHIONE BY AQUEOUS TWO-PHASE EXTRACTION COMBINED WITH TEMPERATURE-INDUCED PHASE SEPARATION |
| |
| Mei lehe,Lin Dongqiang,Zhu Ziqiang.SEPARATION OF GLUTATHIONE BY AQUEOUS TWO-PHASE EXTRACTION COMBINED WITH TEMPERATURE-INDUCED PHASE SEPARATION[J].Journal of Chemical Industry and Engineering(China),1998,49(4):470-475. |
| |
| Authors: | Mei lehe Lin Dongqiang Zhu Ziqiang |
| |
| Abstract: | The techniques for separation of glutathione（GSH）by aqueous two-phase extraction combined with temperature-induced phase separation were studied.The partition behavior of GSH in ethylene oxide-propylene oxide random copolymer(EOPO）/hydroxypropyl starch（PES)／water systems was investigated．The effects of such factors,as concentration of polymer,pH,on the partition coefficients in two separation processes（aqueous two-phase extraction and temperature-induced phase separation)were studied.The optimum conditions were EOPO4000 13%,PES100 10％,pH=10.5. The separation technique for GSH from yeast cell was also investigated.A feasible separation scheme was suggested,and the results show that the total extraction yield could reach over 80％． |
| |
| Keywords: | aqueous two-phase system temperature-induced phase separation glutathione ethylene oxide-propylene oxide random copolymer |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《化工学报》浏览原始摘要信息 |
|
点击此处可从《化工学报》下载全文 |
