| 基于FUNGuild的镰刀菌根腐病发病烟株根际真菌群落研究 |
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| 引用本文： | 李朋发,杨龙,李桂龙,徐后娟,王玉军,李忠佩.基于FUNGuild的镰刀菌根腐病发病烟株根际真菌群落研究[J].中国烟草学报,2019,25(2):63-68. |
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| 作者姓名： | 李朋发 杨龙 李桂龙 徐后娟 王玉军 李忠佩 |
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| 作者单位： | 1.中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室, 南京市北京东路71号 210008 |
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| 基金项目： | 国家自然科学基金项目41771298国家重点基础研究发展计划（973）项目2015CB150501 |
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| 摘 要： | 为了解镰刀菌根腐病发病烟株与未发病烟株在根际真菌群落结构与功能上的差异，联合应用高通量测序和FUNGuild技术研究了镰刀菌根腐病发病烟株与未发病烟株根际土壤中的真菌群落，结果表明:（1）镰刀菌根腐病发病烟株根际真菌群落的Shannon指数为4.14，而未发病烟株则高达4.71，未发病烟株根际真菌群落多样性显著高于发病烟株，镰刀菌根腐病发病烟株与未发病烟株根际土壤中的真菌群落在组成上有显著差异；（2）镰刀菌根腐病发病烟株根际真菌以病理营养型为主，占54.64%，而未发病烟株根际真菌则以腐生营养型为主，占46.10%；（3）镰刀菌根腐病发病烟株根际的病原菌相对丰度为30.73%，显著高于未发病烟株的1.12%，镰刀菌在病原菌群落中占绝对优势地位。高通量测序与FUNGuild联用可对土传病害感病烟株的根际优势病原真菌群落进行准确鉴定，可为烟草病害的防控提供科学参考。
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| 关 键 词： | 烟草 镰刀菌根腐病 根际 真菌 功能 FUNGuild |
| 收稿时间： | 2018-08-05 |
Research of rhizosphere fungi communities of Fusarium root rot diseased tobacco based on FUNGuild |
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| Affiliation: | 1.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China2.University of Chinese Academy of Sciences, Beijing 100049, China3.College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China |
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| Abstract: | In this study, high-through sequencing and FUNGuild were jointly used to investigate the difference in structure and functions of rhizosphere fungi communities between unaffected and Fusarium root rot diseased tobaccos. The results showed: 1) the structure of rhizosphere fungi communities between unaffected and Fusarium root rot diseased tobacco was significantly different, and the alpha diversity of rhizosphere fungi communities of unaffected tobacco was significantly higher than that of diseased tobacco; 2) pathotroph predominated the rhizosphere fungi communities of Fusarium root rot diseased tobacco (54.64%) while saprotroph dominated the rhizosphere fungi communities of unaffected tobacco (46.10%); 3) The relative abundance of rhizospheric pathogen was 30.73% in rhizosphere fungi communities of Fusarium root rot diseased tobacco, which was significantly higher than that of unaffected tobacco (1.12%). In addition, Fusarium species was dominant in the pathogen communities. The co-application of high-through sequencing and FUNGuild is useful in making a comprehensive identification of rhizogenic dominated pathogenic fungi communities of soil-borne diseased tobacco, which provides a scientific reference for tobacco disease control. |
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