Responses of MAC‐T Cells to Inhibited Stearoyl‐CoA Desaturase 1 during cis‐9, trans‐11 Conjugated Linoleic Acid Synthesis |
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| Authors: | Tao Wang Honggu Lee Yuguo Zhen |
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| Affiliation: | 1. College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street no. 2888, Nanguan District, Changchun, Jilin Province, People’ Republic of China;2. Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, Jilin Agricultural University, Xincheng Street No. 2888, Nanguan District, Changchun, Jilin Province, People’ Republic of China;3. Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, 120 Neungdong‐ro, Gwangjin‐gu, Seoul, South Korea;4. JLAU‐Borui Dairy Science and Technology Research & Development Center, Jilin Agricultural University, Xincheng Street no. 2888, Nanguan District, Changchun, Jilin Province, People’ Republic of China |
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| Abstract: | Cis‐9‐conjugated, trans‐11‐conjugated linoleic acid (CLA) is known for its positive activities on human health. The synthesis of cis‐9, trans‐11 CLA in mammary glands is generally thought to be catalyzed by stearoyl‐CoA desaturase 1 (SCD1), but this has not been rigorously established. In this study, we hypothesized that the inhibition of SCD1 (by CAY10566) would block the synthesis of cis‐9, trans‐11 CLA in bovine mammary alveolar cells (MAC‐T) cells. Results showed that MAC‐T cells incubated with 10 nM CAY10566 for 12 h (CAY) produced less cis‐9, trans‐11 CLA (p < 0.01), lower 14:1/(14:1 + 14:0)% (p < 0.01), more trans‐11 18:1 (TVA) accumulation (p < 0.01), and reduced SCD1 mRNA levels (p < 0.01) compared with the control group (CON). Moreover, the mRNA abundances of sterol regulatory element‐binding protein 1 SREBPF1], acyl‐CoA synthetase short‐chain family member 2 ACSS2], and lipin 1 LPIN1] were significantly elevated when SCD1 was inhibited in the CAY group (p < 0.05). Taken together, CAY10566 inhibition of SCD1 resulted in lower cis‐9, trans‐11 CLA synthesis ability, and SREBF1, ACSSS2, and LPIN1 were negatively associated with SCD1. These findings not only provide the direct evidence that cis‐9, trans‐11 CLA synthesis is catalyzed by SCD1, but also help us understand the responses of MAC‐T cells to SCD1 inhibition. |
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| Keywords: | cis‐9 trans‐11 CLA CAY10566 Stearoyl‐CoA desaturase 1 MAC‐T cells |
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