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黄孢原毛平革菌选择性合成木质素过氧化物酶和锰过氧化物酶
引用本文:李华钟,章燕芳,华兆哲,陈坚.黄孢原毛平革菌选择性合成木质素过氧化物酶和锰过氧化物酶[J].过程工程学报,2002,2(2):137-141.
作者姓名:李华钟  章燕芳  华兆哲  陈坚
作者单位:江南大学生物工程学院工业生物技术教育部重点实验室,江苏,无锡,214036
摘    要:在黄孢原毛平革菌(Phanerochaete chrysosporium)的限氮浸没式培养中,研究了不同条件对选择性合成木质素过氧化物酶(LiP)和锰过氧化物酶(MnP)的作用. LiP只在很窄的氮源浓度范围内(0.8~1.8 mmol/L)才能测到,而MnP在较宽浓度范围内(0.4~1.8 mmol/L)表现出较高的酶活水平. 培养基中缺乏Mn2+不能合成MnP,但可以得到活力较高的LiP. Mn2+]在0.06~0.84 mmol/L时可获得LiP. 添加微量Mn2+即可获得较高活力的MnP,此后增加Mn2+对MnP的活力影响不大,直至浓度为3.36 mmol/L才表现出明显的抑制作用. 通纯氧可使LiP活力提高50%,但对MnP影响不大.

关 键 词:黄孢原毛平革菌  木质素过氧化物酶  锰过氧化物酶  选择性合成
文章编号:1009-606X(2002)02-0137-05
修稿时间:2001年7月16日

Selective Synthesis of Lignin Peroxidase and Manganese Peroxidases by Phanerochaete chrysosporium
LI Hua-zhong,ZHANG Yan-fang,HUA Zhao-zhe,CHEN Jian.Selective Synthesis of Lignin Peroxidase and Manganese Peroxidases by Phanerochaete chrysosporium[J].Chinese Journal of Process Engineering,2002,2(2):137-141.
Authors:LI Hua-zhong  ZHANG Yan-fang  HUA Zhao-zhe  CHEN Jian
Abstract:Selective synthesis of lignin peroxidase (LiP) and manganese peroxidase (MnP) by Phanerochaete chrysosporium in N-limited submerged cultures was studied under different physiological conditions. Whereas lignin peroxidase was detectable only in a narrow range of nitrogen source concentration (0.8~1.8 mmol/L), manganese peroxidase reached considerable levels over a broad range (0.4~2.0 mmol/L). In the absence of Mn2+, only lignin peroxidase was detectable, which was also found when the concentration of Mn2+ was in the range of 0.06~0.84 mmol/L. High level of manganese peroxidase was obtained at very low concentration of Mn2+, and almost kept constant with the increment of Mn2+, but was repressed obviously when the concentration of Mn2+ was beyond 3.36 mmol/L. Pure oxygen increased the activity of lignin peroxidase by 50%, but had no obvious effect on manganese peroxidase.
Keywords:Phanerochaete chrysosporium  lignin peroxidase  manganese peroxidase  selective synthesis  
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