首页 | 官方网站   微博 | 高级检索  
     

十二烷基硫酸钠对角质细胞基因表达的影响及作用通路研究
引用本文:赵 丹,苏 宁,张佳婵,李 萌,霍 彤,王昌涛.十二烷基硫酸钠对角质细胞基因表达的影响及作用通路研究[J].日用化学工业,2016,46(6).
作者姓名:赵 丹  苏 宁  张佳婵  李 萌  霍 彤  王昌涛
作者单位:北京工商大学理学院,北京 100048
基金项目:中国检验检疫科学研究院基本科研业务费装箱资金资助项目(2015JK015);质检公益性行业科研专项项目(201310132,201410019)
摘    要:利用人全基因表达谱芯片对十二烷基硫酸钠(SDS)刺激后的角质细胞总RNA进行检测,研究SDS刺激对角质细胞基因表达的影响及作用通路,并对可能引发的疾病进行预测。SDS刺激引起角质细胞中605个基因表达显著上调,368个基因显著下调。主要通过TNF信号通路进行信号传导,经分析筛选出10个关键差异表达基因,包括IL-6,TNF,CCL5,CCL20,CXCL8,CXCL3,CSF2,CSF1,TNFAIP3和NLRC4,以上基因表达异常会促进炎症的发展并激活免疫系统。SDS刺激可能引发特应性皮炎并对过敏与自身免疫疾病有促进作用。

关 键 词:十二烷基硫酸钠  角质细胞  刺激  基因芯片  炎症

Study with respect to effect of sodium dodecyl sulfate on gene expression of keratinocyte and its signaling pathway
Abstract:Detection of total RNA of keratinocytes after irritation of sodium dodecyl sulfate (SDS) was conducted utilizing human mRNA micro array to study the effect of SDS on gene expression of the keratinocytes and its signaling pathway, as well as to predict the possibility of inducing disease by the SDS. Results showed that expression of 605 genes are significantly up regulated and 368 genes are significantly down regulated after exposing to SDS. The signals are transformed mainly by TNF signaling pathway. Ten critical genes (IL-6, TNF, CCL5, CCL20, CXCL8, CXCL3, CSF2, CSF1, TNFAIP3 and NLRC4) are screened by the pathways. Abnormal expression of these genes will affect the development of inflammation and activation of immunity. Possibly, irritation of SDS may cause atopic dermatitis as well as promotion of allergies and autoimmune disease.
Keywords:sodium dodecyl sulfate  keratinocyte  irritation  micro array  inflammation
本文献已被 CNKI 等数据库收录!
点击此处可从《日用化学工业》浏览原始摘要信息
点击此处可从《日用化学工业》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号