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Optimization of Rhizopus niveus Lipase Partitioning by an Aqueous Biphasic System |
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| Authors: | Dayal Aradhana Harihara Puthran Sreeja Govindasamy Sharmila Chandrasekaran Muthukumaran |
| Affiliation: | 1. Bioprocess Laboratory, Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, Tamilnadu, India.;2. Department of Industrial Biotechnology, Government College of Technology, Coimbatore, Tamilnadu, India. |
| Abstract: | An unconventional aqueous two‐phase system (ATPS) composed of polyethylene glycol (PEG) and sodium carbonate was developed and optimized by employing response surface methodology for separation of Rhizopus niveus lipase. A five‐level central composite design was applied to evaluate the optimal level of three process variables in order to obtain maximum lipase separation. Experimental data were analyzed by regression and a polynomial model was created which was found significant. The maximum partition coefficient was achieved with the system PEG 4000/sodium carbonate. Validation experiments confirmed the high accordance of predicted and experimental results. The optimized ATPS can be applied as a suitable cost‐effective system for lipase extraction. |
| Keywords: | Aqueous two‐phase system Lipase Partition coefficient Response surface methodology |
