| F/F对嗜盐污泥以乙酸钠为底物生产PHB能力的影响 |
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| 引用本文： | 崔有为,冀思远,卢鹏飞,张宏宇.F/F对嗜盐污泥以乙酸钠为底物生产PHB能力的影响[J].化工学报,2015,66(4):1491-1497. |
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| 作者姓名： | 崔有为 冀思远 卢鹏飞 张宏宇 |
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| 作者单位： | 北京工业大学环境与能源工程学院, 北京 100124 |
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| 基金项目： | 国家自然科学基金项目(51178004,51478011);北京市自然科学基金项目(8132013);北京市教育委员会面上项目(KM201210005007)~~ |
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| 摘 要： | 嗜盐混合菌生产PHB具有较好的成本优势。本研究接种入海口底泥发展嗜盐活性污泥,利用嗜盐活性污泥以两步法工艺生产PHB。在连续300 d的试验中从长期试验、发酵生产PHB以及表观动力学等方面探索了不同盛宴期/饥饿期(F/F)下筛选菌群的PHB积累能力。研究发现F/F作为重要的参数直接影响嗜盐活性污泥的PHB积累能力。在高F/F下非PHB积累菌群成为优势菌,而低F/F下促进高聚PHB嗜盐菌的选择和富集。在F/F≤0.33条件下筛选的嗜盐活性污泥最大PHB含量可以达到细胞干重的46.7%,PHB产率为0.358 mg PHB·(mg Ac)-1。研究结果将对进一步实现嗜盐污泥生产PHB提供有益的指导。
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| 关 键 词： | 聚-b-羟基脂肪酸酯 嗜盐活性污泥 两步法 PHA合成菌 菌群选择 生物过程 生物技术 数值分析 |
| 收稿时间： | 2014-07-30 |
| 修稿时间： | 2014-12-22 |
Influence of F/F on PHB production by halophilic activated sludge fed by sodium acetate |
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| CUI Youwei;JI Siyuan;LU Pengfei;ZHANG Hongyu.Influence of F/F on PHB production by halophilic activated sludge fed by sodium acetate[J].Journal of Chemical Industry and Engineering(China),2015,66(4):1491-1497. |
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| Authors: | CUI Youwei;JI Siyuan;LU Pengfei;ZHANG Hongyu |
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| Affiliation: | School of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China |
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| Abstract: | Poly-b -hydroxybutyrate (PHB) production by halophilic mixed consortia has great advantage in cost. PHB production by the two-step production process using halophilic activated sludge cultured from estuary sediment was investigated. During the continuous 300 d, the ability of PHB storage at different F/F was assessed from the performance of the long-term tests, experiments of PHB production and the observed kinetics. The ratio of feast to famine (F/F) as an important parameter directly affected the capacity of the PHB-storing of halophilic activated sludge. At a high F/F the non PHB-storing bacteria became dominant in the enrichment reactor, while a low F/F was in favor of the selection and enrichment of PHB-storing bacteria. Moreover, the culture selected at F/F less than 0.33 showed the best PHB-storing capacity with a PHB content of 46.7% cell dry weight and a PHB yield of 0.358 mg PHB·(mg Ac)-1. The results obtained in the study provided a guidance to the production of PHB by halophilic activated sludge. |
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| Keywords: | poly-b-hydroxybutyrate (PHB) halophilic activated sludge two-step production process PHA- storing bacteria culture selection bioprocess biotechnology numerical analysis |
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