| SBBR反应器中耐冷微生物的驯化与识别 |
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| 引用本文： | 吴涵,陈滢,刘敏,王淑莹,张伟.SBBR反应器中耐冷微生物的驯化与识别[J].化工学报,2020,71(2):766-776. |
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| 作者姓名： | 吴涵 陈滢 刘敏 王淑莹 张伟 |
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| 作者单位： | 1.四川大学建筑与环境学院，四川 成都 610065;2.北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室，北京 100124;3.四川大学高分子研究所高分子材料工程国家重点实验室，四川 成都 610065 |
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| 基金项目： | 中央高校基本科研业务费专项;实验室开放基金;国家级大学生创新创业训练计划项目 |
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| 摘 要： | 以模拟生活污水为处理对象，三种不同填料为生物膜载体，运行 A、B、C三个SBBR，通过逐步降温（25、20、15、10、6和5℃）培养驯化处理低温污水的生物膜。运行251周期，三个反应器COD出水浓度均低于50 mg/L；B反应器对氨氮去除效果优于A和C。特别在低温5℃时，A、B和C三个反应器氨氮平均出水浓度分别为14.1、3.79和14.1 mg/L。高通量测序结果显示，5℃时，三个反应器中均以降解有机物的微生物为主；只有B反应器中驯化富集出了嗜冷硝化菌（Candidatus Nitrotoga）；而其他有硝化作用的菌属在B反应器中的丰度也高于A和C反应器；不利于脱氮的类固氮菌（Elstera）在A和C反应器中丰度较高。此结果可从微生物角度解释三个反应器处理效果的差异性，表明B反应器处理低温污水有显著优势，其所用填料可结合低温菌进一步开发利用。
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| 关 键 词： | 废水 生物膜 低温 高通量测序 微生物群落结构 |
| 收稿时间： | 2019-07-01 |
| 修稿时间： | 2019-07-31 |
Domestication and identification of cold-resistant bacteria in SBBR reactor |
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| WU Han,CHEN Ying,LIU Min,WANG Shuying,ZHANG Wei.Domestication and identification of cold-resistant bacteria in SBBR reactor[J].Journal of Chemical Industry and Engineering(China),2020,71(2):766-776. |
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| Authors: | WU Han CHEN Ying LIU Min WANG Shuying ZHANG Wei |
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| Affiliation: | 1.College of Architecture and Environment, Sichuan University, Chengdu 610065, Sichuan, China;2.National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China;3.State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute at Sichuan University, Chengdu 610065, Sichuan, China |
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| Abstract: | Taking simulated domestic sewage as the treatment object, three different fillers are biofilm carriers, and three SBBRs of A, B and C are operated, and the biofilm of low temperature sewage is domesticated and treated by gradual cooling (25, 20, 15, 10, 6 and 5℃). During the 251 operating cycles, the COD effluent concentration of the three reactors was less than 50 mg/L. The ammonium removal efficiency of the B reactor was better than A and C. At 5℃, the ammonium concentration of effluent of A, B and C were 14.1, 3.79 and 14.1 mg/L, respectively. The results of high-throughput sequencing showed that the dominant microbial species in SBBR were mainly those can degrade organic matter at 5℃. Psychrophilic nitrifying bacteria (Candidatus Nitrotoga) were found only in B reactor. The abundance of other common nitrifying bacteria in the B reactor is higher than which in the A and C reactors. The nitrogen-fixing bacteria (Elstera), which adverse to remove ammonium，has the minimal distribution in the B reactor. These results can explain the difference of the pollutant removal of the three reactors from point of view of microorganism, which shows that B reactor has significant advantages in treating low temperature wastewater. |
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| Keywords: | waste water biofilm low temperature high throughput sequencing microbial community structure |
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