| 含水量对氧化铝柱色谱法同时分离纯化卵磷脂和脑磷脂的影响 |
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| 引用本文： | 司朝勇,吴素芳,许海丹,吕秀阳.含水量对氧化铝柱色谱法同时分离纯化卵磷脂和脑磷脂的影响[J].化工学报,2005,56(4):689-693. |
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| 作者姓名： | 司朝勇 吴素芳 许海丹 吕秀阳 |
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| 作者单位： | 浙江大学材料与化工学院，浙江 杭州 310027 |
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| 基金项目： | 浙江省科技计划 (2003C31047)，教育部优秀青年教师资助计划 (教人司 [2002] 40号) 项目.~~ |
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| 摘 要： | 系统地研究了固定相初始含水量和流动相含水量对氧化铝柱色谱法同时分离纯化卵磷脂和脑磷脂的影响.结果表明：在实验范围内，氧化铝的初始含水量对氧化铝柱色谱法同时分离纯化卵磷脂和脑磷脂有很大的影响，而流动相含水量则影响不大.氧化铝初始含水量在8%(质量分数)和流动相含水量在0.1%～1.1%(体积分数)范围内，卵磷脂和脑磷脂可实现基线分离，PC的回收率可达90%以上，PE的回收率可达40%以上.并对实验结果的重现性进行了考察.研究结果除了对卵磷脂和脑磷脂的精制有一定的指导作用外，对其他天然产物分离纯化的研究和生产也有一定的借鉴意义.
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| 关 键 词： | 氧化铝 柱色谱法 卵磷脂 脑磷脂 含水量 |
| 文章编号： | 0438-1157(2005)04-0689-05 |
| 收稿时间： | 2004-2-10 |
| 修稿时间： | 2004-6-15
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Effects of water content on simultaneous purification of PC and PE by alumina column chromatography |
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| SI Chaoyong,WU Sufang,XU Haidan,L Xiuyang.Effects of water content on simultaneous purification of PC and PE by alumina column chromatography[J].Journal of Chemical Industry and Engineering(China),2005,56(4):689-693. |
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| Authors: | SI Chaoyong WU Sufang XU Haidan L Xiuyang |
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| Affiliation: | SI Chaoyong,WU Sufang,XU Haidan,L(U) Xiuyang |
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| Abstract: | The effects of initial water content in alumina and water content of mobile phase on the simultaneous purification of phosphatidyl choline(PC) and phosphatidyl ethanolamine(PE) by alumina column chromatography were studied systematically.The results showed that the initial water content in alumina had great influence on the purification process, but only little changes could be seen for the water content of mobile phase within the experiment range.Baseline separation and higher recovery of PC could be reached at 8%(mass) initial water content in alumina and 0.1%—1.1%(vol) water content of mobile phase.The recovery of PC was more than 90% and the recovery of PE over 40%.Explanation and discussion of the results were given.In addition, reproducibility of the experiments was tested. Not only could the work give some guides in the preparation of high purity PC and PE, but also it would be very helpful in R&;D of other natural products. |
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| Keywords: | alumina column chromatography phosphatidyl choline phosphatidyl ethanolamine water content |
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