| 荧光免疫层析试条光电信号处理及特征量选取 |
| |
| 引用本文： | 高跃明,李天麒,林传阳,潘少恒,韦孟宇,杜民.荧光免疫层析试条光电信号处理及特征量选取[J].电子测量与仪器学报,2015,29(5):662-668. |
| |
| 作者姓名： | 高跃明 李天麒 林传阳 潘少恒 韦孟宇 杜民 |
| |
| 作者单位： | 1. 福州大学物理与信息工程学院 福州 350108; 福州大学福建省医疗器械和医药技术重点实验室 福州 350002
2. 福州大学福建省医疗器械和医药技术重点实验室 福州 350002; 澳门大学科技学院电机及电脑工程系 澳门特别行政区 999078 |
| |
| 基金项目： | 科技部台港澳合作，福建省产学合作重大 |
| |
| 摘 要： | 荧光免疫层析定量检测技术是一种正在兴起的即时检验技术。在前期荧光免疫层析定量检测仪的研究基础上,采用小波算法对A/D采样后的荧光信号进行去噪处理,选用中值滤波剔除基线漂移,最后比较了采用测试线峰值和面积作为特征量的定量检测效果。数据显示,去噪后信噪比提高10 d B,相对均方差减小30.4%;采用峰值和面积值作为定量特征量的测量CV值分别为5.1%和0.4%,测量误差分别为9.1%和0.9%。结果表明采用积分面积作为特征量对荧光信号进行定量检测具有更好的准确度和重复性。
|
| 关 键 词： | 免疫层析 定量检测 特征量 基线漂移 |
Fluorescent immune chromatographic strip signal processing and feature selection |
| |
| Gao Yueming,Li Tianqi,Lin Chuanyang,Pan Shaoheng,Wei Mengyu,Du Min.Fluorescent immune chromatographic strip signal processing and feature selection[J].Journal of Electronic Measurement and Instrument,2015,29(5):662-668. |
| |
| Authors: | Gao Yueming Li Tianqi Lin Chuanyang Pan Shaoheng Wei Mengyu Du Min |
| |
| Affiliation: | 1.College of Physics and Telecommunication Engineering, Fuzhou University
2.Key Lab of Medical Instrumentation and Pharmaceutical Technology of Fujian Province
3.Department of Electrical and Computer Engineering, Faculty of Science and Technology, University
of Macau |
| |
| Abstract: | Immunofluorescence chromatographic quantitative detection technology is an emerging technique of the point of care test (POCT). Based on the previous work of immunofluorescence quantitative chromatography detector, the wavelet algorithm was used to denoise the fluorescence signal after the A/D sampling in this paper. Then the baseline drift was removed by the median filter method. Finally, the peak height and the area were sellected as the characteristic value for the quantitative detection. The experimental data shown that the SNR improved 10dB and the SRMS reduced by 30.4% after denoising. In the quantitatively detect step, the CV were 5.1% and 0.4%, and the test error were 9.1% and 0.9%, for the peak hight and area characteristic value, respectively. The results show that the peak area of the test line as the characteristic value has better accuracy and repeatability than the peak height. |
| |
| Keywords: | immune-chromatographic quantitative detection characteristic value baseline drift |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《电子测量与仪器学报》浏览原始摘要信息 |
|
点击此处可从《电子测量与仪器学报》下载全文 |
|
