Standing Surface Acoustic Wave (SSAW)‐Based Fluorescence‐Activated Cell Sorter |
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| Authors: | Liqiang Ren Shujie Yang Peiran Zhang Zhiguo Qu Zhangming Mao Po‐Hsun Huang Yuchao Chen Mengxi Wu Lin Wang Peng Li Tony Jun Huang |
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| Affiliation: | 1. Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA, USA;2. Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC, USA;3. Key Laboratory of Thermo‐Fluid Science and Engineering, Ministry of Education, Xi'an Jiaotong University, Xi'an, Shaanxi, China;4. Ascent Bio‐Nano Technologies, Inc., Research Triangle Park, NC, USA;5. C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA |
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| Abstract: | Microfluidic fluorescence‐activated cell sorters (μFACS) have attracted considerable interest because of their ability to identify and separate cells in inexpensive and biosafe ways. Here a high‐performance μFACS is presented by integrating a standing surface acoustic wave (SSAW)‐based, 3D cell‐focusing unit, an in‐plane fluorescent detection unit, and an SSAW‐based cell‐deflection unit on a single chip. Without using sheath flow or precise flow rate control, the SSAW‐based cell‐focusing technique can focus cells into a single file at a designated position. The tight focusing of cells enables an in‐plane‐integrated optical detection system to accurately distinguish individual cells of interest. In the acoustic‐based cell‐deflection unit, a focused interdigital transducer design is utilized to deflect cells from the focused stream within a minimized area, resulting in a high‐throughput sorting ability. Each unit is experimentally characterized, respectively, and the integrated SSAW‐based FACS is used to sort mammalian cells (HeLa) at different throughputs. A sorting purity of greater than 90% is achieved at a throughput of 2500 events s?1. The SSAW‐based FACS is efficient, fast, biosafe, biocompatible and has a small footprint, making it a competitive alternative to more expensive, bulkier traditional FACS. |
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| Keywords: | acoustofluidics fluorescence‐activated cell sorters sheathless focusing standing surface acoustic waves |
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