| 基于高通量测序的雪茄烟叶细菌多样性与功能预测 |
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| 引用本文： | 闫铁军,郑霖霖,李秀妮,刘利平,潘勇,王剑,龙飞,周平,时向东.基于高通量测序的雪茄烟叶细菌多样性与功能预测[J].中国烟草学报,2022,28(4):125-132. |
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| 作者姓名： | 闫铁军 郑霖霖 李秀妮 刘利平 潘勇 王剑 龙飞 周平 时向东 |
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| 作者单位： | 1.湖北中烟工业有限责任公司，湖北 武汉 430048 |
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| 基金项目： | 黄鹤楼叶束式手卷雪茄原料生产技术研究2019420000340640 |
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| 摘 要： | 背景和目的 环境条件与烟叶理化性质的改变直接影响烟叶的细菌群落结构和功能。为探究海南不同产区茄衣、茄套和茄芯细菌群落组成，研究雪茄烟叶细菌群落结构及代谢功能。 方法 以海南光村、昌江、白沙和五指山4个产区茄衣、茄套和茄芯为试验材料，基于16S rRNA测序技术分析细菌群落结构，PICRUSt预测分析细菌群落的代谢功能。 结果 12个样本中共聚类2317个OTU，属于10个科。五指山产区茄套的细菌群落丰富度及多样性最高，其Chao1指数、ACE指数和Shannon指数分别为344.00、344.00和6.54。光村和五指山产区细菌群落丰富度及多样性均表现为茄衣 < 茄套、茄芯。昌江产区则表现为茄衣 > 茄芯、茄套。茄衣的主要优势菌科为葡萄球菌科，相对丰度明显高于茄套、茄芯，不同产区之间无明显差异。茄套和茄芯的主要优势菌科为肠杆菌科，明显高于茄衣，且昌江、五指山 > 光村、白沙。KEGG代谢通路分析结果表明，不同产区茄衣、茄套和茄芯细菌预测功能种类差异较小，丰度差异较大。 结论 在科分类水平上，不同产区不同用途雪茄烟叶细菌群落多样性丰富，细菌群落结构组成相似，相对丰度有所差异，葡萄球菌科、肠杆菌科是造成烟叶细菌群落结构差异的关键菌科。细菌群落代谢途径丰富，主要富集在碳水化合物代谢、氨基酸代谢和能量代谢通路中，除此之外，也较多的富集在辅助因子以及维生素代谢等通路。
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| 关 键 词： | 雪茄烟叶 高通量测序 细菌群落组成 功能预测 |
| 收稿时间： | 2021-11-02 |
Diversity analysis and function prediction of bacterial communities in cigar leaves based on high-throughput sequencing technology |
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| Affiliation: | 1.China Tobacco Hubei Industrial Co., Ltd., Wuhan 430048, China2.Tobacco College of Henan Agricultural University, Zhengzhou 45000, China3.Sichuan Agricultural University, Chengdu 610000, China |
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| Abstract: | Background The change of environmental conditions and the physical and chemical properties of tobacco leaves directly affects the structure and function of tobacco leaf bacterial communities. The purpose of this study was to explore the composition of bacterial communities in cigar wrapper leaves, case leaves and core leaves in different producing areas of Hainan, and to study the bacterial community structure and metabolic function of cigar tobacco. Methods 16S rRNA sequencing technology was adopted to analyze the bacterial community structure, and PICRUSt was employed to predict the metabolic profiles of the bacterial community. Results (1) Alpha diversity analysis results show that 12 samples clustered a total of 2317 OTUs, belonging to 10 sections. The diversity and richness of bacterial communities in the aubergine tobacco leaves in the five-fingered mountain production area were the highest, with the values of Chao1, ACE and Shannon indexing 344.00, 344.00 and 6.54, respectively. (2) The analysis of the structure of the flora shows that the main dominant bacteria on the surface of the cigar wrapper leaves are Staphylococcaceae, the main dominant bacteria on the surface of the cigar case and cigar core leaves are Enterobacteriaceae and Pseudomonadaceae, respectively; (3) The results of KEGG metabolic pathway analysis show that the main enrichment pathways of microorganisms on the surface of tobacco leaves in different producing regions are consistent, mainly in carbohydrate metabolism, amino acid metabolism, energy metabolism and metabolism of cofactors and vitamins. Conclusion At the level of family classification, the bacterial community diversity is abundant in different cigar tobacco leaves from different producing areas with different uses, and the bacterial community structure composition is similar, but the relative abundance is different., wherestaphylococcaceae and enterobacteriaceae are the key bacteria causing differences in the structure of tobacco leaf bacterial community. Bacterial community is rich in metabolic pathways, mainly enriched in carbohydrate metabolism, amino acid metabolism and energy metabolism pathways, in addition, also enriched in cofactor and vitamin metabolism pathways. |
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