| 外源添加麝香草酚提高烟草幼苗抵御盐胁迫机理的研究 |
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| 引用本文： | 宋佳倩,徐亮,王悦霖,叶协锋,钟钏,李均,钟子正,姚鹏伟,王静,卢剑.外源添加麝香草酚提高烟草幼苗抵御盐胁迫机理的研究[J].中国烟草学报,2021,27(2):65-71. |
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| 作者姓名： | 宋佳倩 徐亮 王悦霖 叶协锋 钟钏 李均 钟子正 姚鹏伟 王静 卢剑 |
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| 作者单位： | 1.河南农业大学烟草学院, 国家烟草栽培生理生化研究基地, 烟草行业烟草栽培重点实验室, 郑州市金水区文化路95号 450002 |
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| 基金项目： | 国家自然科学基金资助项目“豫中农田土壤改良对生态系统碳收支及净温室效应影响研究”41907076烟草行业烟草栽培重点实验室项目“烤烟土壤肥力培育及提高肥料利用率技术研究”30800665 |
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| 摘 要： | 目的 土壤中盐分含量过高严重影响植物生长，引起盐胁迫。为探寻可以提高烟草耐盐性的外源缓解物质，课题组前期筛选了大量化合物，选用缓解效果最优的麝香草酚进行耐盐胁迫机理的研究。 方法 以烤烟K326为材料，对烟草根尖进行根尖细胞死亡的鉴定和钠钾离子流速的测定，并结合烟草幼苗根长、活性氧含量、抗氧化酶含量等生理指标，探究麝香草酚缓解烟草幼苗盐胁迫的机理。 结果 （1）添加麝香草酚显著降低了盐胁迫对烟草幼苗根系生长的抑制。（2）麝香草酚降低了盐胁迫下植株体内H2O2和MDA的积累、缓解了盐胁迫造成的根尖细胞死亡。（3）麝香草酚缓解了盐胁迫造成的Na+吸收和K+外排。 结论 外源添加麝香草酚可以通过降低H2O2造成的氧化损伤，维持细胞内钠钾离子平衡来缓解盐胁迫对烟草幼苗的伤害。
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| 关 键 词： | 麝香草酚 盐胁迫 活性氧 细胞死亡 非损伤微测技术度 |
| 收稿时间： | 2020-09-24 |
Study on the mechanism of exogenous thymol to improve tobacco seedlings' resistance to salinity stress |
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| Affiliation: | 1.Key Laboratory for Tobacco Cultivation of Tobacco Industry, National Tobacco Cultivation & Physiology & Biochemistry Research Center, College of Tobacco, Henan Agricultural University, Zhengzhou 450002, China2.Sichuan Province Liangshan Prefecture Huidong County Tobacco Company, Sichuan 615200, China3.Sichang Branch of Liangshan Tobacco Company of Sichuan Province, No. 368 Sanchakou East Road, Xichang City, Liangshan Yi Autonomous Prefecture 615000, China |
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| Abstract: | Objective High salt content in soil seriously affects plant growth and causes salinity stress. In order to explore the exogenous substances that can improve the salt tolerance of tobacco, the research group screened a large number of compounds in the early stage. Thymol with the best alleviation effect was adopted to study the mechanism of salinity stress. Methods Using flue-cured tobacco K326 as material, the cell death of root tip and the flow rate of sodium and potassium ion were determined were measured by chemical staining and non-invasive micromeasurement. Based on physiological indexes such as root length, active oxygen content and antioxidant enzyme content of tobacco seedlings, the mechanism of thymol in alleviating salinity stress in tobacco seedlings was explored. Results Test data show: 1) The addition of thymol significantly declined the inhibition effect of salinity stress on the root growth of tobacco seedlings; 2) Thymol reduced the accumulation of H2O2 and MDA in plants under salinity stress and alleviated the death of root tip cells caused by salt stress. 3) Thymol alleviated Na+ uptake and K+ efflux caused by salt stress. Conclusion Exogenous thymol can alleviate the salt stress-induced damage to tobacco seedlings by reducing the H2O2–induced oxidative damage and maintaining the balance of intracellular sodium and potassium ions. |
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