| 拟粉红锁掷孢酵母降解展青霉素的机制 |
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| 引用本文： | 孙艺文,赵利娜,郑香峰,林珍,李侨飞,程洋洋,张红印.拟粉红锁掷孢酵母降解展青霉素的机制[J].食品与生物技术学报,2020,39(2):16-23. |
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| 作者姓名： | 孙艺文 赵利娜 郑香峰 林珍 李侨飞 程洋洋 张红印 |
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| 作者单位： | 江苏大学 食品与生物工程学院，江苏 镇江 212013;江苏大学 生命科学研究院，江苏 镇江 212013 |
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| 基金项目： | 国家自然科学基金项目(31571899);国家重点研发计划项目(2016YFD0400902-04);江苏省农业科技自主创新资金项目(CX(15)1048)。 |
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| 摘 要： | 展青霉素(PAT)是一类主要由曲霉属和青霉属等真菌产生的有毒次级代谢产物,主要污染水果及其制品,严重威胁着人类的健康。为了寻找一种安全高效的PAT脱毒方法,近年来采用生物法清除PAT已成为主要的研究方向。作者研究了拟粉红锁掷孢酵母(Sporidiobolus pararoseus)对PAT降解的影响及其降解机制。结果显示,S.pararoseus可以显著降低苹果伤口处PAT的积累量,体外实验发现S.pararoseus与PAT(5μg/mL)共同培养18 h,PAT可以被S.pararoseus完全降解。S.pararoseus对PAT的降解作用既不是酵母细胞壁的吸附作用,也不是细胞的吸收作用,而是细胞正常代谢下产生的胞内酶对PAT具有降解作用。本研究有助于了解S.pararoseus降解PAT的作用机制,为真菌毒素的生物降解提供了新的理论依据。
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| 关 键 词： | 展青霉素 拟粉红锁掷孢酵母 生物降解 机制 |
The Possible Mechanisms Involved in Degradation of Patulin by Sporidiobolus pararoseus |
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| SUN Yiwen,ZHAO Lin,ZHENG Xiangfeng,LIN Zhen,LI Qiaofei,CHENG Yangyang,ZHANG Hongyin.The Possible Mechanisms Involved in Degradation of Patulin by Sporidiobolus pararoseus[J].Journal of Food Science and Biotechnology,2020,39(2):16-23. |
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| Authors: | SUN Yiwen ZHAO Lin ZHENG Xiangfeng LIN Zhen LI Qiaofei CHENG Yangyang ZHANG Hongyin |
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| Affiliation: | (School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;Institute of Life Sciences,Jiangsu University,Zhenjiang 212013,China) |
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| Abstract: | Patulin(PAT) is a mycotoxin mainly produced by Aspergillus and Penicillium. PAT contamination worldwide is considered to be a serious health concern. New strategies to prevent or reduce patulin contamination in fruits and derived products are meaningful. Recently,biological approach for PAT detoxification is being explored. In this study,the efficacy of patulin degradation by Sporidiobolus pararoseus and the degradation mechanisms involved were investigated. The findings revealed that S. pararoseus can significantly reduce the accumulation of PAT in apple wounds. PAT(5 μg/mL) was completely degraded at 18h post incubation with S. pararoseus in vitro. Further research found that PAT degradation is independent of the absorption of yeast cells and cell walls. The intracellular enzymes obtained from the S. pararoseus cells showed they are more efficient to degrade PAT than the yeast cells. Our findings contributes to the understanding of the mechanisms of patulin degradation by S. pararoseus,which provided a foundation for the detoxification of patulin in fruits and derived products. |
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| Keywords: | patulin Sporidiobolus pararoseus biodegradation mechanism |
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