Identification of the Major Components of Buddleja officinalis Extract and Their Metabolites in Rat Urine by UHPLC‐LTQ‐Orbitrap |
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| Authors: | Mohan Sun Zhiqiang Luo Yang Liu Ruirui Yang Lina Lu Guohua Yu Xiaoyun Ma Aoxue Liu Yafang Guo Haiyu Zhao |
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| Affiliation: | 1. School of Chinese Materia Medica, Beijing Univ. of Chinese Medicine, Beijing, P.R. China;2. Inst. of Chinese Materia Medica, China Acad. of Chinese Medical Sciences, Beijing, P.R. China |
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| Abstract: | Buddleja officinalis Maxim, one of the most popular herbal medicines in China, is widely prescribed for curing eye diseases for centuries. In this study, the major components of B. officinalis extract (BOE) and their metabolites in rat urine were detected and identified by ultra‐high‐pressure liquid chromatography coupled with linear ion trap‐orbitrap tandem mass spectrometry (UHPLC‐LTQ‐Orbitrap). A total of 19 compounds, including 8 flavonoids and 11 phenylethanoid glycosides, were confirmed or tentatively identified from BOE. In vivo, 33 components, including 3 prototypes and 30 metabolies, were confirmed or tentatively identified in rat urine samples. The metabolic pathways of different types of compounds were also proposed. This study would effectively narrow the range of potentially bioactive constituents of BOE and shed light to its action mechanism. |
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| Keywords: | Buddleja officinalis extract major compounds metabolites UHPLC‐LTQ‐Orbitrap |
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