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超高效液相色谱-串联质谱法测定鸡组织和 牛组织中的甲苄喹啉和癸氧喹酯
引用本文:林 黎,康海宁,肖陈贵,沈金灿,叶 刚.超高效液相色谱-串联质谱法测定鸡组织和 牛组织中的甲苄喹啉和癸氧喹酯[J].食品安全质量检测技术,2014,5(11):3371-3378.
作者姓名:林 黎  康海宁  肖陈贵  沈金灿  叶 刚
作者单位:深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心
基金项目:出入境检验检疫行业标准资助计划(2007B903),国家质检总局科技计划项目(2012IK186)
摘    要:目的 建立超高效液相色谱-串联质谱检测鸡组织和牛组织中甲苄喹啉和癸氧喹酯残留的检测方法。方法 样品采用乙腈提取,亲水-亲脂平衡介质固相萃取柱Hydrophile-Lipophile Balance(HLB) 净化,超高效液相色谱-串联质谱仪检测,外标法定量。结果 甲苄喹啉和癸氧喹酯在0.005~0.250 mg/ kg 范围内线性关系良好,相关系数均大于0.999,方法定量限为0.01 mg/kg,平均回收率范围为77.0%~110%,相对标准偏差范围为1.45%~12.4%。结论 本方法简便、快速、准确、灵敏度高,适用于鸡组织和牛组织中甲苄喹啉和癸氧喹酯残留的检测与确证。

关 键 词:甲苄喹啉、癸氧喹酯、超高效液相色谱-串联质谱法、鸡组织、牛组织
收稿时间:2014/10/9 0:00:00
修稿时间:2014/10/29 0:00:00

Determination of nequinate and decoquinate residues in chicken tissues and bovine tissues by ultra performance liquid chromatography-tandem mass spectrometry
LIN Li,KANG Hai-Ning,XIAO Chen-Gui,SHEN Jin-Can and YE Gang.Determination of nequinate and decoquinate residues in chicken tissues and bovine tissues by ultra performance liquid chromatography-tandem mass spectrometry[J].Food Safety and Quality Detection Technology,2014,5(11):3371-3378.
Authors:LIN Li  KANG Hai-Ning  XIAO Chen-Gui  SHEN Jin-Can and YE Gang
Affiliation:Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau
Abstract:Objective To develop a method for determination of nequinate and decoquinate residues in chicken tissues and bovine tissues by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The residues of nequinate and decoquinate in the test sample were extracted with acetonitrile. The extraction was cleaned with hydrophile-lipophile balance (HLB) cartridge. The residues were determined by UPLC-MS/MS, using external standard method. Results The limits of quantification were 0.010 mg/kg for nequinate and decoquinate, the standard curves were linear in the range of 0.005~0.250 mg/kg, with the correlation coefficients >0.999. The average recovery was between 77.0%~110%, the RSD was between 1.36%~12.4%. Conclusion This method is rapid, simple, precise, and sensitive for both confirmation and quantification of nequinate and decoquinate residues in chicken tissues and bovine tissues.
Keywords:nequinate  decoquinate  ultra performance liquid chromatography  - mass spectrum spectrometry(UPLC-MS/MS)  chicken  tissues  bovine  tissues
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