| 表面增强拉曼光谱法快速测定凉茶中的甲硝唑 |
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| 引用本文： | 林长虹,黄 惠,李小苑,彭 贵,邓琳娜,申红桃,江宛桐,古丽君,伍 聪,江培淳.表面增强拉曼光谱法快速测定凉茶中的甲硝唑[J].食品安全质量检测技术,2023,14(24):177-183. |
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| 作者姓名： | 林长虹 黄 惠 李小苑 彭 贵 邓琳娜 申红桃 江宛桐 古丽君 伍 聪 江培淳 |
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| 作者单位： | 深圳市计量质量检测研究院,深圳市计量质量检测研究院,深圳市计量质量检测研究院,深圳市计量质量检测研究院,深圳市计量质量检测研究院,深圳市计量质量检测研究院,深圳市计量质量检测研究院,深圳市计量质量检测研究院,深圳市计量质量检测研究院,深圳市计量质量检测研究院 |
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| 基金项目： | 广东省市场监督管理局科技项目（2022CZ12） |
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| 摘 要： | 目的 基于表面增强拉曼光谱法(surface enhanced Raman spectroscopy, SERS),建立了一种快速检测凉茶中甲硝唑的方法。方法 以乙腈为提取剂，以十八烷基键合硅胶固定相和N-丙基乙二胺为净化剂对凉茶样品进行提取净化获得凉茶样品待测液。利用光还原原理,在太阳光照下通过甲醇还原四氯金酸合成了金纳米颗粒。将合成的金纳米颗粒作为基底材料,对凉茶样品待测液进行表面增强拉曼光谱分析。结果 在0.05~3.00 g/L范围内,甲硝唑的特征峰强度和浓度具有良好的线性关系,相关系数为0.9991,方法检出限为0.05 g/L,方法成功应用于凉茶样品中甲硝唑的检测,回收率为92.1%～102%,相对标准偏差为3.8%~8.5%。结论 本方法准确度高、操作简单快速,满足凉茶中甲硝唑的快速检测要求,在凉茶中非法添加物现场快速检测方面具有良好的应用潜力。
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| 关 键 词： | 表面增强拉曼光谱 甲硝唑 凉茶 |
| 收稿时间： | 2023/10/10 0:00:00 |
| 修稿时间： | 2023/12/10 0:00:00 |
Rapid detection of metronidazole in herbal tea by surface-enhanced Raman spectroscopy |
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| LIN Chang-Hong,HUANG Hui,LI Xiao-Yuan,PENG Gui,DENG Lin-N,SHEN Hong-Tao,JIANG Wan-Tong,GU Li-Jun,WU Cong,JIANG Pei-Chun.Rapid detection of metronidazole in herbal tea by surface-enhanced Raman spectroscopy[J].Food Safety and Quality Detection Technology,2023,14(24):177-183. |
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| Authors: | LIN Chang-Hong HUANG Hui LI Xiao-Yuan PENG Gui DENG Lin-N SHEN Hong-Tao JIANG Wan-Tong GU Li-Jun WU Cong JIANG Pei-Chun |
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| Affiliation: | Shenzhen Academy of Metrology & Quality Inspection,Shenzhen Academy of Metrology & Quality Inspection,Shenzhen Academy of Metrology & Quality Inspection,Shenzhen Academy of Metrology & Quality Inspection,Shenzhen Academy of Metrology & Quality Inspection,Shenzhen Academy of Metrology & Quality Inspection,Shenzhen Academy of Metrology & Quality Inspection,Shenzhen Academy of Metrology & Quality Inspection,Shenzhen Academy of Metrology & Quality Inspection,Shenzhen Academy of Metrology & Quality Inspection |
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| Abstract: | Objective To establish a rapid method for detecting metronidazole in herbal tea using surface-enhanced Raman spectroscopy (SERS). Methods Using acetonitrile as the extraction agent, octadecyl bonded silica gel fixed phase and primary secondary amines as the purification agent, the herbal tea sample was extracted and purified to obtain the test solution. Additionally, gold nanoparticles were synthesized based on this photoreduction synthesis strategy, achieved by reducing tetrachloroauric acid with methanol under sunlight. These synthesized gold nanoparticles served as the base material for surface-enhanced Raman spectroscopy analysis in herbal tea samples.Results Within the concentration range of 0.05-3.00 g/L, a strong correlation coefficient of 0.9991 emerged between the intensity of the characteristic peak and metronidazole concentration. The method demonstrated an impressive detection limit of 0.05 g/L. Its successful application to real samples yielded a recovery rate ranging from 92.1% to 102% and a relative standard deviation of 3.8% to 8.5%. Conclusion This method stands out for its high accuracy and straightforward, rapid operation. It fulfills the requirements for swiftly detecting metronidazole in herbal tea and exhibits significant potential for on-site, rapid detection of illicit additives in herbal tea. |
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| Keywords: | Surface-enhanced Raman Spectroscopy metronidazole herbal tea |
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