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Residues of different gel formulations on dentinal walls: A SEM/EDS analysis
Authors:Angela Longo do Nascimento  Fernanda Hoffmann Busanello  Marcus Vinícius Reis Só  Milton Carlos Kuga  Jefferson Ricardo Pereira  Fabiana Soares Grecca
Affiliation:1. Department of Conservative Dentistry, Federal University of Rio Grande Do Sul, Porto Alegre, Rio Grande do Sul, Brazil;2. Department of Restorative Dentistry, School of Dentistry, S?o Paulo State University (UNESP), Araraquara, S?o Paulo, Brazil;3. Department of Prosthodontics, Dental School, University of Southern Santa Catarina, Tubar?o, Santa Catarina, Brazil
Abstract:The aim of this study was to evaluate the presence of residues of sodium hypochlorite gel, chlorhexidine gel, and EDTA gel on dentinal walls after canal preparation through chemical SEM‐ elemental chemical microanalysis (EDS) analysis. Forty‐eight single‐rooted teeth were selected. They had their crowns sectioned and were instrumented with a reciprocating system. The canals were irrigated with 5 mL of saline solution during root canal preparation. After instrumentation, the root canals were irrigated with 3 mL 17% EDTA followed by 1 min of ultrasonic passive activation (3× 20 sec) to remove the smear layer, and then irrigated with 3 mL of saline solution. The specimens were randomized into three groups (n = 12) according to the chemical substance that filled the root canal for 30 min: GI: 5.5% sodium hypochlorite gel; GII: 2% chlorhexidine gel; GIII: 24% EDTA gel; Negative control group: no substance was used. Then, the root canals were irrigated with 6 mL of saline solution followed by 1 min of ultrasonic passive activation (3× 20 sec). After ultrasonic activation, the canals were irrigated with 2 mL saline. The roots were sectioned, and the percentage of each chemical element present in the samples was analyzed through chemical SEM‐EDS microanalysis. All experimental groups showed a significantly higher percentage of chemical elements (Na and/or Cl) than the control group (P < 0.03). This in vitro study has shown that, regardless of chemical solutions used even after the final irrigation protocol, chemical residues of different substances remained attached to the root canal walls. Microsc. Res. Tech. 78:495–499, 2015. © 2015 Wiley Periodicals, Inc.
Keywords:endodontics  sodium hypochlorite  chlorhexidine  EDTA  electron probe microanalysis
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