汽车暖风装置放热量微机测试系统

本文介绍了一种汽车暖风装置放热量微机测试系统，该系统采用ＳＴＤ总线工业控制机对汽车用水暖式暖风装置进行自动化测试，测试结果及数据处理结果由ＣＲＴ显示器用汉字和数字显示并可用行式打印机输出有关数据和报表     

化学振荡反应微机自动测试系统

本文介绍了化学振荡反应微机自动测试系统的设计及功能。该测试系统硬件包含四大模块：温度测试和显示模块、化学振荡反应的振荡周期测试和显示模块、电压测试模块、数据上传到计算机的通信模块，系统也可以脱离计算机独立使用。下位机软件采用汇编语言编写，上位机用VisualBasic6．0语言编写。该测量系统可对化学振荡反应实验全过程的数据进行采集和测量，具有使用方便，设计新颖，实验数据稳定，现象直观等特点。     

微机继电器测试系统的研制

介绍了一套２８６微机及单板机组成的测试系统的开发过程，该系统用于批量继电器可靠性和触点接触电阻测试。系统实现了过程测试、故障检测及处理、数据处理的自动化，能实用于生产过程中双列直插式继电器的批量检测，解决人工抽样检测的弊端。系统在实测中取得了满意的结果，具有高可靠性和实用性。     

微机局域网在运载火箭总体测试发射中的应用

本文阐述了运载火箭总体测试发射工作的测试指挥情况，提出了具体的设计方案、硬件软件配置，信息分类，数据传输与交换，并分析了充分利用遥测数据提高发射可靠性，建立纵深立体化的Ｃ＾３Ｉ系统。     

微机控制时间继电器参数测试系统

本文介绍一个以APPLE—I兼容微机系统为测控核心的时间继电器参数自动测试系统的硬件、软件设计和工作原理。该系统只需输入被测试品的型号规格就能自动完成10个被测件的延时值及其重复动作误差、电源波动误差的同时测试,并进行数据统计、质量评判和结果打印。整个测试系统采用总线式结构,具有结构简单,便于维护,工作稳定可靠的优点。     

应用8751单片机和386PC机的电动转辙机测试系统

本文介绍电动转辙机微机测试系统的硬件组成和压力，电压、电流取样和Ａ／Ｄ转换原理以及软件设计思想，该能自动地测试电动转辙机的各种参数，具有工作效率高，数据准确可靠并自动记录存档的特点。     

一个全台压气机实验实时控制,采集,处理系统

传统的压气机实验测试方式已不能满足高性能航空发动机研制的要求，为此，本文介绍了一个全台压气机实验实时控制、采集、处理系统；给出了该系统的硬件结构、实现技术及其实时软件设计。系统由微型计算机控制扫描阀、ＮＥＦＦ－６２０巡检设备、三向位移机构等设备协调运行，使探针自动移位与数据采集、处理同步进行，并以图、表即时输出实验结果。系统投入使用以来，实验时间和成本均降低了５０％以上，测试精度０．３％。     

多功能空调设备性能试验台微机自控系统

本文针对自行研制的多功能空调设备性能试验台微机自控系统，介绍了系统的软、硬件结构，以及相应的测试原理和控制策略。与同类实验系统相比，提高了测试过程的自动化程度和空调设备性能的测试精度。     

微机遥控加油计量系统

本文以俄罗斯引进的尹开德500型电子遥控加油机改进为背景，叙述了微机遥控加油计量系统的原理，软件和硬件构成，给出了系统试验测试结果。     

机动车废气微机测试原理与数据分析

本文介绍机动车微机检测系统中车辆废气排放测试的方法与过程，着重阐述检测对象的数据变化特点及软件的跟踪算法，该方法对于测量范围大，显示精度高的场合有它独到之处，值得推广。     

CAMAC系统在时间飞行质谱测量中的应用

利用ＣＡＭＡＣ系统和微机建立的高速数据采集及传输系统，可以广泛应用于快速时间分辨的微弱信号检测，本文介绍了该系统硬件及软件设计，并给出了利用它测量激光溅射铁氰化钾产生团簇离子的飞行时间质谱（ＴＯＦ－ＭＳ）的实验结果。     

A Multilayer MEMS Platform for Single-Cell Electric Impedance Spectroscopy and Electrochemical Analysis

The fabrication and characterization of a microchamber electrode array for electrical and electrochemical studies of individual biological cells are presented. The geometry was tailored specifically for measurements from sensory hair cells isolated from the cochlea of the mammalian inner ear. Conventional microelectromechanical system (MEMS) fabrication techniques were combined with a heat-sealing technique and polydimethylsiloxane micromolding to achieve a multilayered microfluidic system that facilitates cell manipulation and selection. The system allowed for electrical stimulation of individual living cells and interrogation of excitable cell membrane dielectric properties as a function of space and time. A three-electrode impedimetric system was incorporated to provide the additional ability to record the time-dependent concentrations of specific biochemicals in microdomain volumes near identified regions of the cell membrane. The design and fabrication of a robust fluidic and electrical interface are also described. The interface provided the flexibility and simplicity of a “cartridge-based” approach in connecting to the MEMS devices. Cytometric measurement capabilities were characterized by using electric impedance spectroscopy (1 kHz–10 MHz) of isolated outer hair cells. Chemical sensing capability within the microchannel recording chamber was characterized by using cyclic voltammetry with varying concentrations of potassium ferricyanide $(hbox{K}_{3}hbox{Fe}(hbox{CN})_{6})$. Chronoamperometric recordings of electrically stimulated PC12 cells highlight the ability of the platform to resolve exocytosis events from individual cells.$hfill$[2007-0105]      

Sensitive electrochemical immunosensor based on enlarged and surface charged gold nanoparticles mediated electron transfer

A simple and sensitive electrochemical immunosensor for the detection of protein biomarker interleukin-6 (IL-6) based on gold nanoparticles (AuNPs) as label was reported. The signal of the immunosensor was originated from enlarged and positively charged AuNPs mediated electron transfer between insulating self-assemble monolayer (SAM) modified electrode and K₃Fe(CN)₆ solution. The gold electrode was first modified with SAM to block the electron transfer between the electrode and K₃Fe(CN)₆ solution. After the preparation of the immunosensor, the AuNPs attached onto electrode was enlarged and positively charged when treated in solution containing HAuCl₄, ascorbic acid and cetyltrimethylammonium bromide (CTAB). The enlarged and positively charged AuNPs then mediated electron transfer between the electrode and K₃Fe(CN)₆ solution, creating redox current that is proportional to the concentration of IL-6 detected. The reported immunosensor has high sensitivity and wide linear range. This novel signal amplification strategy can be applied to the detection of different kinds of protein biomarkers.     

Trace detection of nitroaromatic compounds with layer-by-layer assembled {SBA/PSS}n/PDDA modified electrode

The {SBA/PSS}_n/PDDA films modified electrode was prepared by layer-by-layer (LBL) assembly with mesoporous SiO₂ (SBA), poly(sodium 4-styrene-sulfonate) (PSS) and poly(diallyldimethylammonium chloride) (PDDA) in this paper. SBA is a large pore-size mesoporous material with highly ordered hexagonally arranged mesochannels and high thermal stability etc. The electrochemical characteristics of the {SBA/PSS}_n/PDDA films have been studied by electrochemical impedance spectroscopy in 0.1 M KCl solution containing 5.0 mM Fe(CN)₆³⁻/Fe(CN)₆⁴⁻ at the formal potential of 0.230 V. The ultratrace nitroaromatic compounds (NACs) such as TNT, TNB, DNT and DNB were determined by differential pulse voltammetry (DPV) measurement. The sensitivities for NACs determination with {SBA/PSS}_n/PDDA modified electrode were dependent on the number of layers, pH and ionic strength of electrolyte, based on which a set of optimized conditions for film fabrication was inferred. The current responses were linear with NACs ranging from 10⁻⁹ to 10⁻⁷ mol/l. The results showed that the {SBA/PSS}_n/PDDA modified electrode established a new way for fast, simple and sensitive analysis of NACs.     

Electrochemical copper (II) sensor based on self-assembled 4-amino-6-hydroxy-2-mercaptopyrimidine monohydrate

A 4-amino-6-hydroxy-2-mercaptopyrimidine monohydrate (AHMP)-based self-assembled monolayer (SAM) was formed on the gold electrode surface. Ellipsometric measurements evidenced the SAM formation on the gold electrode surface. The structural integrity of the modified gold electrode was also characterized by insulating properties of the SAM that were detected by cyclic voltammetry. The results of cyclic voltammetry showed that the SAM, which was formed by assembly of AHMP, was stable but did not completely block the redox-activity of ferrocene and K₃[Fe(CN)₆]/K₄[Fe(CN)₆]. In contrast completely blocked redox-activity was observed after the treatment of AHMP-based SAM with saturated solution of 4-formylphenylboronic acid in 1,4-dioxan. The modified electrodes exhibited a selective response towards Cu(II) ions in the presence of some interfering ions such as Cd(II), Co(II), Fe(II), Ni(II) and Pb(II). This study is the first scientific report on the application of AHMP-modified electrode as a selective Cu(II) sensor in the presence of some interfering cations.     

Microfabricated electrochemical sensors for exhaustive coulometry applications

The development of microfabricated electrochemical systems suitable for deployment in sensor networks that operate with a minimum of operator intervention are of great interest; therefore, a coulometric sensing system for exhaustive coulometry with the potential for calibration-free operation has been designed, fabricated and evaluated to support such development. The sensor chips were microfabricated onto a silicon substrate and contained a variety of specially designed thin-film gold working electrodes (ranging from one to five per chip) and a Ag/AgCl pseudo-reference electrode. A custom flow cell containing fluidic connections and counter electrode chamber was also constructed to integrate the sensor and to create an electrolysis chamber with a fixed volume. Different chip designs were evaluated as exhaustive coulometric sensors in terms of reproducibility and longevity using Fe(CN)₆^3?/4? as model analytes. The relative standard deviation (RSD) for a chip tested over a period of 42 days was 5.5% whereas the sensor-to-sensor reproducibility was within 6.3%.     

Performance of intelligent Mobile IPv6

Mobile IP is the current standard proposed by IETF for mobility management in IP networks. Mobile node communicating with static correspondent node (CN) has been considered in previous performance studies. We propose the mobility of CN and two additional mobility combinations for Mobile IP in this paper. CN mobility causes performance degradation in an environment with frequent handoffs. A new scheme for Mobile IPv6 called Intelligent Mobile IPv6 is proposed. It is observed through simulation results that the performance of Intelligent Mobile IPv6 is better than Mobile IPv6.     

Monofunctionalized β-cyclodextrins as sensor elements for the detection of small molecules

β-Cyclodextrins functionalized by different moieties that were tethered to a single 6-deoxyaminoglucose unit were investigated with respect to their suitability for sensor applications. Derivatizing the cyclodextrins with hydrophobic moieties like dipalmitoylglycerol and cholesterol allowed us to study packing density and orientation of the cyclodextrin tori at the air–water interface. From the pressure-area isotherms, it was concluded that the cyclodextrins are positioned towards the water subphase, with their molecular axis predominately parallel to the interface. By introducing a disulfide group, we managed to immobilize cyclodextrins on gold surfaces via self-assembly. MALDI mass spectrometry (MALDI MS) and XPS confirmed that the molecules are chemisorbed on the gold substrate displaying high surface coverage as determined by means of impedance spectroscopy. The inclusion of various charged guest molecules was monitored by changes in the charge transfer resistance of the redox couple [Fe(CN)₆]³⁻/[Fe(CN)₆]⁴⁻. The charge transfer resistance is sensitive to the surface potential, which leads to either repulsion or attraction of the redox active species.     

基于抗体包被金磁纳米微粒修饰的磁性安培免疫传感器研制及对人血清癌抗原19-9的检测

在金电极表面修饰壳聚糖(CS)膜,并在酸性pH下利用CS上形成的-NH3+静电吸引Fe(CN)]3-6-(FeCN)到电极表面,制成了在pH 6.5磷酸盐缓冲液(PBS)中具有电活性的Au | CS-FeCN电极;再通过外加磁场吸引,在Au | CS-FeCN表面修饰一层血清癌抗原19-9单克隆抗体(anti CA19-9)包被的金磁纳米微粒(Fe3O4(核)／Au(壳),简称GMP),由此构建了一类快速检测CA19-9的无试剂安培免疫传感器(Au| CS-FeCN／GMP-anti CA19-9).用扫描电镜(SEM),X-射线荧光光谱(XFS)和X-射线光电子能谱(XPS)对电极表面进行了表征;并采用循环伏安法(CV),示差脉冲伏安法(DPV)、交流阻抗法(E1S)分 别研究了该传感器的电化学性质和对CAl9-9的检测性能.实验表明:Au I CS-FeCN／GMP-anti CAl9-9电极在含CAl9-9的pH 6.5 PBS中于35℃下温育25 min,其DPV还原电流下降值(AI)与CA19-9在0.1～10 U／mL和10~50 U／mL范围内成正比,检出下限为0.056 U／mL(3d).用于血清样本检测并和标准ELISA方法对照,结果一致.只需移去外加磁场,用PBS清洗电极表面即可实现电极更新.该磁性免疫传感器集分离、富集和测定于一身,灵敏度高、稳定性好、表面易更新,有望用于人血清中痕量CA19-9的快速检测.     

Impedimetric immunosensor for electronegative low density lipoprotein (LDL) based on monoclonal antibody adsorbed on (polyvinyl formal)-gold nanoparticles matrix

Monoclonal antibodies (MAb) have been commonly applied to measure LDL in vivo and to characterize modifications of the lipids and apoprotein of the LDL particles. The electronegative low density lipoprotein (LDL⁻) has an apolipoprotein B-100 modified at oxidized events in vivo. In this work, a novel LDL⁻ electrochemical biosensor was developed by adsorption of anti-LDL⁻ MAb on an (polyvinyl formal)-gold nanoparticles (PVF-AuNPs)-modified gold electrode. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize the recognition of LDL⁻. The interaction between MAb-LDL⁻ leads to a blockage in the electron transfer of the [Fe(CN)₆]⁴⁻/K₄[Fe(CN)₆]³⁻ redox couple, which may could result in high change in the electron transfer resistance (R_CT) and decrease in the amperometric responses in CV analysis. The compact antibody-antigen complex introduces the insulating layer on the assembled surface, which increases the diameter of the semicircle, resulting in a high R_CT, and the charge transferring rate constant κ⁰ decreases from 18.2 × 10⁻⁶ m/s to 4.6 × 10⁻⁶ m/s. Our results suggest that the interaction between MAb and lipoprotein can be quantitatively assessed by the modified electrode. The PVF-AuNPs-MAb system exhibited a sensitive response to LDL⁻, which could be used as a biosensor to quantify plasmatic levels of LDL⁻.