Total Phosphorus Content in Technologically Unprocessed Meat

The total phosphorus content was measured in raw, technologically unprocessed meats. Our results show that total phosphorus content in different raw meats varies substantially (from 1.41 g/kg to 4.22 ± 0.93 g/kg). Based on the results, manufacturers of meat products are advised that before production of any meat product, the precise amount of total phosphorus in raw meat starting material has to be known. Based on this, the content of added synthetic phosphates and polyphosphates together with the content of natural phosphorus in the meat would be within the prescribed values stated in the Regulation, which are 8 g/kg.     

Total Phosphorus Content in Various Types of Cooked Sausages from the Serbian Market

In the period January 2014 to March 2015, phosphorous levels were measured in 701 samples of different types of cooked sausages from the Serbian market. The highest level of phosphorus (9.52 g/kg, expressed as P₂O₅) was found in fine comminuted sausage, and the lowest (0.94 g/kg) in meat pâté. The most common (33.38%) range of phosphorous levels was 4.01-5.00 g/kg P₂O₅. National Regulation allows total phosphorus content (as P₂O₅) up to 8.00 g/kg. Only three sausages (0.43%) did not meet this requirement, but the rest of the analyzed sausages (99.57%) were safe for consumption, with respect to content of phosphorus.     

Quality of donkey meat and carcass characteristics

A study based on 15 entire donkey males was carried out to evaluate carcass quality and nutritional characteristics of meat obtained by these animals slaughtered at 15 months of age and a mean final body weight of 181 kg. The meat had a low (2.02 g/100 g) fat content, an appreciable (22.8 g/100 g) protein content, and cholesterol content was 68.7 mg/100 g. Glycogen was also determined (0.45 g/100 g) within 12 h of sampling. Potassium was the mineral with the highest content (343 mg/100 g), followed by phosphorus (212 mg/100 g), sodium (52 mg/100 g) and magnesium (24 mg/100 g). Donkey meat obtained from young animals can be considered a good alternative to other red meats and not only for the production salami, or other fermented meat products.     

Physical,Chemical and Sensory Properties of Baked Products from Blends of Wheat and African Yam Bean (Sphenostylis stenocarpa) Water-Extractable Proteins

Blends of wheat flour (WF) and African yam bean water-extractable proteins (AYBWEP) were processed into bread and cookies in the following ratios: 100: 0; 95: 5; 90: 10; 85: 15; 80: 20. The proximate composition, physical, chemical properties and sensory properties of bread and cookies samples from the blends were determined. Breads and cookies produced from the resultant blends were significantly higher (p < 0.05) in protein (16.39% – 18.36%) than the control (11.80% – 12.58%). Carbohydrate content decreased from 60.74% with addition of AYBWEP to 52.81% following 20% substitution. The pH of bread samples prepared from whole wheat flour and blends of wheat flour and AYBWEP were significantly different (p < 0.05) while bulk density and specific volume were not significantly different (p > 0.05). The pH of bread samples and cookies decreased with increase in the proportion of the AYBWEP blend from 5% to 20%. The highest specific volume (3.70 ml/g) was observed in bread samples prepared from the control 100: 0 blends while the 80:20 blends had the lowest specific volume (3.10 ml/g). There was no significant difference (p > 0.05) in the bulk density and thickness of the cookies. The cookies prepared using 80: 20 blends had the higher diameter (22.53 cm) and spread factor (54.03 cm) compared to the control. Generally, acceptability of the bread and cookies decreased with higher ratios of AYBWEP inclusion. The sensory acceptability scores showed the best AYBWEP substitution level for making bread and cookies was 5% and 10% of the AYBWEP respectively. The results are discussed in the context of the growing importance of promoting the processing and utilization of lesser known local crops in baked products.enrichment.     

Growth of Listeria Monocytogenes in Traditional Austrian Meat Jelly Products

Individual components of a traditional meat jelly (cooked meat chunks, gelatin and preboiled vegetable) with differences in pH and a_w can constitute a niche for the multiplication of Listeria. Listeria monocytogenes counts remained stable in jelly over 21 days at 2 and 8 °C, whereas in meat and vegetables, a >1 log₁₀ unit increase was observed after 7 days at 2 °C (or >5 log₁₀ at 8 °C). In the composed product, Listeria numbers remained stable at 2 °C (21 days), but increased more than 1 log₁₀ during 7 days at 8 °C. Improving safety of jellied meat by lowering pH is discussed.     

Effect of Sources of Fibre on Performance of Growing Snail

The proximate and physicochemical properties of cassava leaf and peel meals were evaluated with a view to possible replacement of wheat offal which is the conventional source of fibre in animal feed, with these meals. The effect of feeds produced with cassava leaf and peel meals on the performance of growing snails was also investigated. Feeds (F1, F2 and F3) were formulated to contain 240, 235 and 230 g/kg cassava root meal each and 85, 85 and 90 g/kg cassava peel meal, wheat offal meal and cassava leaf meal respectively. The formulated feeds contain approximately 18.0% crude protein, 7.5% ash, 3% fat, 6.0% crude fibre 8%, calcium, 0.7% phosphorus, and energy level of 2400 kcal ME / kg. A total of 54 growing snails (Archachatina marginata) were used to investigate the nutritive potential of the formulated feeds on performance of growing snails for 15 weeks. Concentrations of the crude protein, crude fat, crude fibre, ash and calcium in cassava leaf meal were higher than those of wheat offal and cassava peel meal, with the exception of nitrogen free extract which was highest (70.01%) in cassava peel meal. Feed intake was 576 g 569 g and 581 g for snails fed with cassava leaf meal, cassava peel meal and wheat offal respectively but the corresponding weight gain ranged between 123.35 and 134.81 % being highest for F1. The feed conversion ratio shows that F1 > F3 > F2 indicating better conversion of feed to edible meat in F1. The results show that cassava leaves and peels have a strong potential to substitute the traditional wheat offal and can therefore be adapted as commercial feed ingredients.     

The advantage of using extrusion processing for increasing dietary fibre level in gluten-free products

Gluten-free products generally are not enriched/fortified and frequently are made from refined flour and/or starch. Such products have been found to provide lower amounts of total dietary fibre than their enriched/fortified gluten-containing counterparts.The objective of this study was to increase the level of total dietary fibre in gluten-free products by using extrusion technology and by incorporating a number of different fruits and vegetables, such as apple, beetroot, carrot, cranberry and gluten-free Teff flour cereal. The materials were added at the level of 30% into the gluten-free balanced formulation (control) made from rice flour, potato starch, corn starch, milk powder and soya flour. Different process conditions, such as water feed rate 12%, solid feed rate 15–25 kg/h, screw speed 200–350 rpm, barrel temperatures: 80 °C at feed entry and 80–150 °C at die exit were used. Pressure, material temperature and torque were monitored during extrusion runs. The relationships and interactions between raw ingredients, extrusion processing parameters and resulting extrudate nutritional and textural properties were investigated.The results of this study clearly show that extrusion technology has the potential to increase the levels of total dietary fibre in gluten-free products made from vegetables, fruits and gluten-free cereals.     

Effect of Soy Flour and Maize Flour Addition on Phase Separation in Moi-Moi from Soaked Cowpea (Vigna unguiculata) and Cowpea Flour from Different Cowpea Varieties

Two improved varieties of cowpea (IT89KO and IT90K-76) and one local variety (lsiocha) were used to investigate the effects of added soyflour and maize flour on the phase separation in moi-moi made from soaked cowpea and cowpea flour. Different levels (5%, 10%, 15%, 20% and 25%) of maize flour, soyflour and a 1:1 blend of soyflour and maize flour (soy/maize flour) were separately added. The moi-moi from the different combinations was evaluated for phase separation and per cent height of the upper layer calculated. Soaking of cowpea reduced the size of the upper layer in moi-moi compared with the use of cowpea flour. The addition of maize flour or the soy/maize flour reduced the upper layer compared with when either flour was added alone. The upper layer of moi-moi made from cowpea flour with added soy flour (21.20%) was significantly higher (p < 0.05) than moi-moi from soaked cowpea with added soy flour (15.2%). Moi-moi with added maize flour made from cowpea flour produced a significantly (p < 0.05) higher mean per cent upper layer (19.72%) compared with that from soaked cowpea (8.95%). Addition of soyflour produced the greatest upper layer for all varieties used. There were no significant (p > 0.05) differences in the per cent upper layers for moi-moi from all cowpea varieties when maize flour was added. Increasing the proportion of added flours increased the size of the upper layer. Complete prevention of the occurrence of phase separation in moi-moi by addition of soy flour and maize flour alone is not feasible.     

Preliminary Studies of the Chemical Composition and Sensory Properties of Sweet Potato Starch-Wheat Flour Blend Noodles

Preliminary studies of chemical composition and sensory properties of instant noodles from blends of wheat flour and sweet potato starch were carried out. Sweet potato starch was used to replace wheat flour at 30, 40, 50, 60 and 70%. Proximate, vitamin A, mineral analysis and sensory evaluation were carried out by standard methods. Results showed that the formulated noodles had higher carbohydrate (63.34 – 70.53%), moisture (4.34 – 4.97%) and vitamin A (11.62 – 35.00 mg/100 g) but lower protein (3.36 – 7.89%), fat (16.91 – 25.09%), calcium (0.73 – 0.89%), phosphorus (0.24 – 0.32%), iron (0.10 – 0.27%) and ash content (1.17 – 3.17%) than the commercial noodles from wheat flour. The noodles containing 30% sweet potato starch showed no significant difference (p > 0.05) from commercial noodles (100% wheat flour) in terms of colour, crunchiness, taste and general acceptability, with improved nutrient composition. It is concluded that production and consumption of wheat flour/potato starch blend noodles should be encouraged to increase the calorie and vitamin A intake in the diet, especially for children who are the major consumers of noodles; apart from helping to promote and improve the utilization of sweet potato tubers.     

Incorporation of bovine dry blood plasma into biscuit flour for the production of pasta

Spray-dried blood plasma (DBP) (10.9 g/100 g [w/w] nitrogen) was added to medium-protein biscuit flour (1.4 g/100 g N) during pasta manufacture. High-protein durum semolina (2.0 g/100 g N) was used to produce the control pasta. Sensory data indicated that the addition of DBP produced pasta with significantly better colour intensity and acceptability, aroma intensity, flavour intensity, textural strength, texture acceptability, aftertaste intensity, aftertaste acceptability, and overall acceptability. The DBP/biscuit flour formulation that gave the optimum balance between pasta protein content and organoleptic acceptability contained 2.2 g/100 g DBP. A higher content of DBP resulted in increased protein levels, but these pasta formulations were less acceptable organoleptically.     

Enrichment of Noodles with Soy Flour and Carrot Powder

Noodles were produced from four flour blends of whole wheat, wheat-cassava, wheat-cassava-soy flour and wheat-cassava-carrot flour blends respectively. The flours were analyzed for proximate analysis, functional properties and total carotenoid content. The cooking time of all samples was also determined. Results show that there were significant differences (p < 0.05) between values obtained for moisture content, protein and total carbohydrates (p < 0.05) while values for fat and ash contents of the samples were not significantly different (p > 0.05). The functional properties also showed significant differences (p < 0.05) between the pasting properties except for pasting temperature and the cooking time of the samples. The total carotenoid content of the dried carrot sample was found to be 28.34 mg/100 g dry weight basis, while the noodle sample containing 10% dried carrot sample (CSC4) had a total carotenoid content of 1.80 mg/100 g dry weight basis. The results suggest that noodles made from the different flour blends can compare favourably with conventional noodles made from wheat flour in quality and that carrot flour can be used for noodle enrichment.     

Chemical,functional and nutritional properties of wet dehulled niger (Guizotia abyssinica Cass.) seed flour

Niger seeds were dehulled using hot lye treatment. Dehulling of niger seeds increased the protein and fat contents from 24 to 35 and 31–53 g/100 g and decreased the crude fiber from 16.9 to 2.2 g/100 g, respectively. The defatted flour prepared from dehulled niger seeds was evaluated for chemical, functional and nutritional properties and compared to the undehulled flour. The protein content of the dehulled flour increased from 44 to 63 g/100 g. Dehulling resulted in inactivation of trypsin inhibitor activity. The dehulled flour had higher water and fat absorption capacities. However, the nitrogen solubility, emulsification capacity and foaming properties decreased. Chemical score of the dehulled flour was higher; threonine was the first limiting amino acid followed by lysine and isolecuine. The in vitro digestibility of the protein increased due to dehulling (85.5%) compared to that of undehulled flour (76%). The calculated nutritional indices, essential amino acid index, biological value, nutritional index and C-PER, were higher in dehulled flour compared to undehulled flour. The available lysine content appeared to be unchanged.     

Bioactivity of ellagic acid-, lutein- or sesamol-enriched meat patties assessed using an in vitro digestion and Caco-2 cell model system

Interest exists in the manufacture of functional meat products whereby synthetic antioxidants are replaced with naturally-sourced compounds. Therefore the aim of this study was to determine the bioactivity of pork and beef patties containing ellagic acid (600 μg/g), lutein (200 μg/g) or sesamol (500 μg/g). Cooked pork and beef patties were subjected to an in vitro digestion procedure and the resultant micelles were added to human intestinal Caco-2 cells. Supplementation with micelles from lutein-enriched pork patties protected (P < 0.05) against H₂O₂-induced cell injury whereas the presence of control beef, lutein-enriched beef or ellagic acid-enriched beef, at levels ?20% (v/v), enhanced (P < 0.05) oxidant-induced cytotoxicity. None of the pork patties significantly modulated cellular glutathione content. Micelles from all the enriched pork patties significantly protected against H₂O₂-induced DNA damage. In conclusion, the cytoprotective and genoprotective effects of ellagic acid, lutein, and sesamol, when incorporated into meat systems, depend greatly on the food matrix.     

Pithecellobium jiringa legume flour for potential food applications: Studies on their physico-chemical and functional properties

Jering seeds are traditionally consumed as a legume in South-East Asia. In the present study, we evaluated the physico-chemical, functional and cooking properties of jering seed flour with a major aim to popularise this seed for development of new food products. The seed flour had high moisture (58.55%) and crude protein contents (14.19%). Results on the functional properties revealed significant differences in the protein solubility, which showed a decrease from pH 2 to pH 4, with high water absorption (2.83 ml/g) and oil absorption (1.83 ml/g) capacities. The emulsifying activity and stability of the seed flour was highest at 0.4 M (26.27% and 75.75%, respectively). Addition of carbohydrates (lactose, maltose and sucrose) decreased the least gelation capacity. Results of this study showed functional properties of jering seed flour to be dependent on the concentration, pH and ionic strengths, which can be suitably modified for development of new food products.     

Cold atmospheric pressure plasma processing of insect flour from Tenebrio molitor: Impact on microbial load and quality attributes in comparison to dry heat treatment

In this study, the applicability of semi-direct cold atmospheric pressure plasma (CAPP) during postharvest processing of Tenebrio molitor flour is investigated. Besides analyzing the decontamination efficacy, plasma-induced impact on techno-functionality, protein solubility, composition and structure was determined and compared to heat induced effects.Following CAPP treatment, the total microbial load of the Tenebrio flour of 7.72 log₁₀ cfu/g was reduced to 7.10 (1 min), 6.72 (2.5 min), 5.79 (5 min), 5.19 (7.5 min), 5.21 (10 min) and 4.73 (15 min) log₁₀ cfu/g. With increasing exposure to CAPP, protein solubility at pH 4 almost linearly decreased to a minimum of 54%. Water binding capacity decreased from 0.79 to 0.64 g_water/g whereas oil binding capacity increased from 0.59 to 0.66 g_oil/g. Gel electrophoresis revealed a decrease of all protein fractions at pH 4 whereas at pH 10 the band pattern significantly shifted to protein fractions with higher molecular weights.Industrial relevanceEdible insects are rich in valuable protein, fat, fibre, minerals and micronutrients. Although a wide range of species represent a valuable alternative protein source that could contribute to food and feed security, they are industrially hardly exploited. The tailored application of proper processing technologies could lead to novel insect-based high-protein food and feed products with unique functional properties supporting the increase in acceptability among potential consumers. Current research concentrates on developing processing chains including innovative nonthermal approaches. Cold atmospheric pressure plasma (CAPP) has gained attention as an effective technology for the decontamination and modification of fresh and dry agricultural products. In the postharvest chain of edible insects, the application of CAPP could contribute to the development of safe and high-quality insect-based products in the food and feed sector.     

Bread Making Potential of Composite Flour of Wheat-Acha (Digitaria exilis staph) Enriched with Cowpea (Vigna unguiculata L. walp) Flour

Bread-making potentials of composite flours containing 90% wheat and 10% acha enriched with 0-15% cowpea flour were investigated. Proximate composition and functional properties of the blends were studied using AOAC standard methods. Bread loaves were prepared from the blends using the straight dough method and evaluated for loaf height, loaf volume, loaf weight and sensory characteristics. Crude protein, crude fat, crude fibre and ash contents increased significantly (p < 0.05) with increase in level of cowpea flour addition, but moisture content was not significantly (p > 0.05) different among the blends. Functional properties, with exception of bulk density and swelling capacity, were significantly (p < 0.05) different among the blends. Average loaf height and loaf volume decreased significantly (p < 0.05) with increased cowpea flour but loaf weight showed opposite trend with significant (p < 0.05) differences as cowpea flour increased. However, the addition of cowpea flour significantly (p < 0.05) decreased the loaf specific volume but all enriched samples were not significantly (p > 0.05) different. Bread samples from composited blends were rated lower than bread from all wheat bread. Bread loaves from enriched composite flour with up to 10% cowpea flour were acceptable to the panelists.     

Physicochemical Properties of Mango Seed Flour

The proximate composition, functional and antinutritional properties of flour produced from seeds of mangoes grown in Ebonyi State were studied. Two cultivars: India and Indochinese were used for the study. The aim of the study was to determine any possible potential that flour obtained from these seeds might possess. Results revealed that flours from India and Indochinese cultivars had protein contents of 6.00 and 4.95%; moisture, 5.00 and 10.36%; fibre, 11.00 and 15% while the carbohydrate was 74.41 and 64.23% respectively. Results from the functional properties showed that flours from both of the cultivars had water absorption capacities of 2.0 g/g and 1.5 g/g; oil absorption capacity of 2.16 g/g and 1.83 g/g while foaming capacity was 3.79 g/g and 3.75 g/g with India cultivar having higher values for each of the functional properties studied although, there was no significant difference (p > 0.05) in the values except for the water absorption capacity. Levels of hydrogen cyanide, phytic acid and trypsin inhibitors were generally low and within safe levels.     

Desorption isotherms of salted minced pork using K-lactate as a substitute for NaCl

The aim of this study was to obtain and compare water desorption isotherms of ground meat containing NaCl (0.107 kg NaCl/kg raw-meat dry matter) and/or K-lactate as NaCl substitute at two different levels of molar substitution (30% and 100%). A thin layer of salted ground meat was dried and sampled at pre-determined times. The moisture content of the samples and their water activities (a_w) were measured at 5 °C and 25 °C. Results showed that ground meat with NaCl and/or different K-lactate contents had a similar water desorption isotherm for a_w ranging from 0.7 to 1.0. Below 0.7, the water equilibrium content fell with small decreases in a_w faster for meat with NaCl than for meat with K-lactate. K-lactate could reduce the excessive hardening at the surface of salted meat products. Experimental desorption isotherms were compared to those estimated using two approaches of the Ross equation. Models provided a good fit for the experimental data.     

Physical,Sensory and Microbiological Properties of Wheat-Fermented Unripe Plantain Flour

The physical, sensory and microbiological properties of wheat-fermented unripe plantain composite flour bread were studied. Mature unripe plantain was peeled, sliced, steam blanched, dried and milled into flour. The flour was made into a slurry (10 g of flour/3 ml of water) and allowed to ferment for 24 h. It was then dried, pounded and sieved through 0.25 mm sieve. The fermented unripe plantain flour was then blended with wheat flour in the ratios of (wheat:fermented unripe plantain) 100:0; 90:10; 80:20; 70:30; and 60:40. Bread was produced from the flour blends using the straight dough method. Loaf weight and volume decreased significantly (p < 0.05) with increasing levels of plantain flour inclusion. Sensory evaluation of the flour samples revealed significant differences in the ratings for crumb colour and texture between 100% wheat flour (100:0) and 60% wheat-40% fermented unripe plantain flour (60:40) bread but no significant difference was observed between all bread samples with respect to crust colour, taste, aroma and overall acceptability. The sensory scores showed that all the bread samples were acceptable. Microbiological analysis (total viable count) revealed that all the bread samples were free of microorganisms for up to four days after production.     

Physicochemical and Sensory Properties of Instant Kunun-Zaki Flour Blends from Sorghum and Mango Mesocarp Flours

Instant Kunun-zaki, a fermented non-alcoholic sorghum beverage, was prepared by mixing different per cent blend ratios of unmalted sorghum flour: mango mesocarp flour (90:0, 75:15, 70:20, 65:25, and 60:30) with 10% malted sorghum. Proximate compositions, chemical and functional properties of the blends were analyzed. Addition of mango mesocarp flour significantly (p ≤ 0.05) increased the ash (1.31 to 1.75%), crude fibre (2.57 to 3.39%) and decreased significantly (p < 0.05) the energy content (368.21 to 354.67 kcal/100 g) of the blends. The β-carotene content also increased from 95.65 to 139.13 μg/100 g with increased mango mesocarp flour. Hygroscopicity increased significantly (p < 0.05) from 6.10 to 10.28% while viscosity of the blends decreased significantly (p < 0.05) from 1715 to 1195.46 cP. Mango mesocarp flour addition increased the ash, crude fibre and introduced β-carotene into the product.