Growth performance of rats fed raw and extruded African breadfruit‐based complementary diets: A response surface analysis

BACKGROUND: African breadfruit, from which diverse food forms are produced, is popular in most West African tropical regions. The present study is part of a larger research project aimed at developing appealing, processed, nutritious products from blends of breadfruit, corn and defatted soybean by utilising extrusion cooking using response surface analysis. The study describes the ability or otherwise of raw and extruded African breadfruit‐based diets, similar to weaning diets simulated with rats, to support growth of children and infants. RESULTS: Maximum protein efficiency and net protein ratios of 2.22 and 3.69, respectively, were obtained with rats fed diets made from 40 g 100 g^?1 African breadfruit + 5 g 100 g^?1 corn + 55 g 100 g^?1 soybean extrudates at 18 mL 100 mL^?1 feed moisture and 140 rpm screw speed. It compared favourably with a casein standard and was 54–69% and 42–55% higher than those fed raw diets. CONCLUSION: Extrusion cooking significantly improved the nutritional quality of African breadfruit–corn–defatted soybean as reflected in their protein efficiency and net protein values. The superiority in protein quality of diet 1 (containing 40 g 100 g^?1 breadfruit, 5 g 100 g^?1 corn and 55 g 100 g^?1 defatted soybean) over the other experimental diets has serious nutritional implication in dietary diversification to address protein‐energy malnutrition prevalent in Nigeria and other West African countries especially where African breadfruit is known to be highly cultivated as a food resource. Copyright © 2007 Society of Chemical Industry     

Production of maize–bambara groundnut complementary foods fortified pre‐fermentation with processed foods rich in calcium,iron, zinc and provitamin A

BACKGROUND: Maize–bambara groundnut complementary foods are deficient in calcium, iron, zinc and vitamin A. Food‐to‐food fortification could be cheaper, safer and more easily adopted by local communities compared to the use of chemically pure compounds and vitamins to enrich such foods. RESULTS: Maize–bambara groundnut complementary foods fortified for iron, zinc, calcium and vitamin A by blending with a multi‐mix (1.41:1:2.25, w/w) of processed roselle calyces, cattle bones, and red palm oil in a 1:2.1 (w/w) ratio showed significant increases in calcium, iron, zinc and vitamin A contents of 3.26–4.225, 0.083–0.134 and 0.015–0.017 g kg^?1 and 4855.3–7493.7 µgRE kg^?1, respectively. CONCLUSION: The maize–bambara groundnut foods had calcium, iron, zinc and vitamin A contents that satisfy the proposed nutrient requirements for infants. Only the maize–bambara groundnut and maize–bambara groundnut malt fermented by backslopping [(MB)_b and (MB_m)_b] containing red palm oil emulsified with Brachystegia eurycoma had calcium contents significantly (P < 0.05) higher than Nutrend, a complementary food produced by Nestle (Nigeria) PLC. These products are from raw materials produced in commercial quantities by rural farmers using household level technologies which the rural and urban poor can more easily access in order to reduce micronutrient malnutrition. Copyright © 2010 Society of Chemical Industry     

Viscoelastic properties and pasting characteristics of fermented maize: influence of the addition of malted cereals

Investigations were conducted on the viscoelastic properties and pasting characteristics of fermented maize with malted cereals and their suitability for infant feeding. A 3 × 3 × 2 factorial experimental design with malting time, cereal malt concentration and cereal type was used. Maize, millet, and sorghum malts were added to fermented maize to reducing its bulk density. Samples were analysed for their viscoelastic properties and pasting characteristics using Brabender Viscoamylograph. Sorghum malt in comparison to millet and maize malts was not effective in terms of lowering the hot and cold paste viscosities of the fermented maize. Maize and millet malts liquefied the dough considerably during both the hot and cold paste viscosities. The effect of 4‐day malted millet was most pronounced whilst the highest activity of maize malt was observed with the 3‐day malted flours. The addition of maize and millet malts to fermented maize were most effective in lowering the viscoelastic properties of the resulting porridges.     

Pasting,thermal, gel texture,resistant starch and colour properties of unripe banana flour from 10 desert banana varieties cultivated in South Africa

Unripe banana flour is a potential commercial ingredient in various food products for increased resistant starch and reduced gluten contents. In the present study, the pasting (rapid visco-analysis), gel texture (penetration test), thermal (differential scanning calorimetry), colour (tri-stimulus colour indices) and the resistant starch properties of unripe banana flour produced from different dessert banana varieties (n?=?10) cultivated in South Africa, were analysed and juxtaposed to wheat and maize flour. The functional properties varied significantly (p?≤?0.05) between banana varieties, and from wheat and maize flours, to various extents. Selected functional property ranges of unripe banana, wheat and maize flours, respectively included; flour colour index (63.16–76.42, 77.34 and 80.96), paste viscosity (405.5–556.6, 124.7 and 115.6 RVU), gelatinization temperature (64.67–71.21, 71.11 and 69.95?°C), gel firmness (7.24–11.44?×?10^??2 N, 3.49?×?10^??2 N and 6.56?×?10^??2 N) and resistant starch content (19.9–47.4, 2.8 and 2.2% w/w). Multivariate analysis (principle component analysis) showed that the unripe banana flours from different varieties were distinguished from each other based on the pasting temperature. The unripe banana flours were distinguished from both wheat and maize flour based on breakdown and peak paste viscosities. The breakdown viscosity was the most positively related measure to the resistant starch content with a linear regression R-squared value of 0.898, indicating a significant role played by granule structure in resistance to enzymatic hydrolysis. The present research demonstrates that selection of appropriate dessert banana variety is important when replacing staple flours (wheat and maize) with unripe dessert banana flour as a functional ingredient.     

Composition of Ghanian fermented maize meal and the effect of soya fortification on sensory properties

There were no significant differences in the composition of four fermented maize doughs used by different ethnic groups in Ghana. The average maize dough contained 49.6% moisture, 37.5% starch and had a titratable acidity of 4.3 mg NaOH g^?1 and pH of 3.76. Fermented maize dough (48% m.c.) was fortified with defatted soya flour at 50, 100, 150 and 200 g kg^?1 on a replacement basis and used in two traditional food products. Triangle taste tests indicated a significant difference between the control and samples fortified with 10% or more soya flour. Based on a preference test, maize dough products with up to 10% added soya flour were acceptable to Ghanaians.     

Cultivation,preparation and consumption of ensete (ensete ventricosum) in Ethiopia

In Ethiopia almost 10 million people are dependent on ensete (Ensete ventricosum (Welw) Cheesrnan), also known as ‘false banana’. In the Gurage area in Central Ethiopia, agronomic and nutritional aspects of ensete were studied in 60 households in six villages. Ensete is propagated vegetatively and has a 6-year growing cycle during which it is transplanted three or four times. Men harvest the plants; women scrape the pseudo stem in order to separate the starchy pulp from the fibre, and pulverise the corm. The pulp is fermented and stored for up to 5–7 years in earthen pits. The yield of ensete food (ko'cho) was found to be 34 kg per plant or 9.5 tons ha ^?1 per year. Compared with other foods grown in Ethiopia, the energy yield of ensete (6.1 MJ m ^?2 per year) was higher than that of all cereals, Irish potato, sweet potato and banana, but lower than that of cassava. The protein yield of ensete was higher (11 ± 4gm^?2 per year) than all of the crops mentioned above, except for banana and Irish potato. To make ensete bread, fermented pulp is squeezed to make it drier, chopped to shorten the fibres and a 2 cm layer is baked for 15 min. Unfermented freshly harvested corm is also eaten after boiling. All foods have a low protein content (4–22 gkg^?1). Bu'lla, white desiccated juice collected from the pulp, is more energy rich (8.5 MJ kg^?1) than ko'cho (6.5 MJkg^?1). A dietary survey, conducted in 39 households comprising 237 persons, showed that the average daily intake of 0.55 kg ensete provided 68% of total energy intake, 20% of protein, 28% of iron but no vitamin A. Energy intake from all food consumed was very low, being only 60% of requirements, while protein intake at 107% was ample. Since ensete can be stored for years, is readily available throughout the year and can withstand dry periods, its cultivation can significantly improve household food security in highland areas prone to drought and famine.     

Effect of soaking,dehulling, cooking and fermentation with Rhizopus oligosporus on the oligosaccharides,trypsin inhibitor,phytic acid and tannins of soybean (Glycine max Merr.), cowpea (Vigna unguiculata L. Walp) and groundbean (Macrotyloma geocarpa Harms)

Soybean, cowpea and groundbean, three locally grown legumes in West Africa, were processed into tempe, an Indonesian-type fermented food. Changes in oligosaccharides, trypsin inhibitor, phytic acid and tannins were monitored during the pretreatments (soaking and soaking–dehulling–washing–cooking) and fermentation with Rhizopus oligosporus. About 50% of raffinose and more than 55–60% of sucrose and stachyose were lost during the pretreatments of the beans. Stachyose decreased during fermentation with a reduction of 83.9%, 91.5% and 85.5% respectively for soybean, cowpea and groundbean while raffinose remained fairly constant. Galactose, the predominant sugar, glucose, fructose, maltose and melibiose increased during the first 30 and 36 h of fermentation of cowpea and groundbean, but decreased thereafter. Soaking the beans for 12–14 h had no effect on the level of trypsin inhibitor of the beans while it increased the phytic acid content to 1.7% in soybean and to 0.8% and 0.7% in groundbean and cowpea. Cooking soaked and dehulled soybean, cowpea and groundbean for 30, 7 and 15 min respectively resulted in 82.2%, 86.6% and 76.2% in trypsin inhibitor. However, a slight increase in trypsin inhibitor was observed during soybean fermentation. Phytic acid decreased during fermentation by 30.7%, 32.6% and 29.1% respectively in soybean, cowpea and groundbean at the harvesting time. Tannins mainly located in seed coat were removed as a result of pretreatments mainly dehulling. These changes are beneficial especially in infant feeding based on cereal and legume-based foods.     

Increased quality and functionality of kimchi when fermented in Korean earthenware (onggi)

Kimchi was fermented in onggi and other containers at 4 °C for 4 weeks. Two types of onggi were used: glazed and nonglazed onggi. Other containers such as polyethylene plastic containers, polypropylene containers for use in a kimchi refrigerator, stainless steel and glass bottles were used. During the fermentation, kimchi fermented in onggi showed relatively stable acidity change compared with those in the other containers. Also it showed higher lactic acid bacteria multiplication (10⁸–10⁹ cfu g^?1 at 4th week) but slower general aerobic bacteria multiplication than the others. The springiness of kimchi fermented in onggi was more than 50% at 4th week, it is higher than the others. Its carbonic acid taste and overall acceptability were excellent in sensory evaluations. The antioxidative and cancer‐cell antiproliferative activities were also greater in kimchi fermented in onggi than the others. Between the two types of onggi, nonglazed onggi showed better results in both properties and functionalities of kimchi. Onggi showed higher gas permeability than polyethylene plastic containers and glass bottles because of its porous structure, and lactic acid bacteria could have benefited from this condition. It possibly contributes to optimal ripening of kimchi resulting in better properties, especially texture and taste, and health functionalities.     

Ripening temperature affects the content and distribution of isoflavones in sufu,a fermented soybean curd

Sufu, a fermented soybean curd, was prepared by ripening the salted tofu cubes in the Aspergillus oryzae‐fermented rice–soybean koji mash at 25, 35 or 45 °C for a period of 16 days. It showed that the 16‐day ripened sufu contains less total isoflavone content (629.29–739.68 μg g^?1 dried matter) than the salted tofu before ripening (942.59 μg g^?1 dried matter). Regardless of ripening temperature, ripening causes a major reduction in the content of β‐glucoside and malonylglucoside isoflavones along with a significant increase of aglycone isoflavone content. These changes were enhanced as the ripening period extended. Among the various treatments examined, sufu ripened at 45 °C showed the greatest increase in aglycone content coupled with the greatest decrease in malonylglucosides. The distribution of malonylglucosides decreased from an initial 40.32% in the tofu cube to 9.78% after 16 days of ripening at 45 °C. Meanwhile, the distribution of aglycone increased from 13.17% to 39.88%.     

Effect of milk fat replacement by polyunsaturated fatty acids on the microbiological,rheological and sensorial properties of fermented milks

A modified milk (W3DD) where fat had been replaced by oils enriched in ω‐3 polyunsaturated fatty acids was used for the manufacture of a set‐type fermented product. In order to improve the organoleptic properties of the product, 30 g l^?1 whey protein concentrate (WPC) was added during the manufacturing process. Samples were fermented employing a commercial probiotic starter culture (ABT‐2), which contained Streptococcus thermophilus ST‐20Y, Lactobacillus acidophilus LA‐5 and Bifidobacterium lactis BB‐12. The acidification process was dependent on the WPC addition, which favoured the increase of viable counts, but fermentation was not influenced by the milk fat composition. The highest counts of the probiotic strains, L acidophilus LA‐5 (3.3 × 10⁵ cfu g^?1) and B lactis BB‐12 (5.5 × 10⁷ cfu g^?1), after 21 days of storage at 4 °C, were found in fermented products derived from W3DD supplemented with WPC. Addition of WPC also increased the firmness of the products and reduced syneresis. No apparent colour changes due to fat composition or WPC supplementation were observed in the products. Milk fat replacement by oils rich in ω‐3 polyunsaturated fatty acids had a negative influence on the product texture but did not affect the typical yoghurt flavour. These defects were overcome by the addition of 30 g l^?1 WPC, which improved the appearance, texture and general acceptability scores in the product. Copyright © 2004 Society of Chemical Industry     

Analysis and risk assessment of ethyl carbamate in various fermented foods

Ethyl carbamate (EC) was analyzed in fermented foods, comprising various matrices; subsequently, a risk assessment for EC was carried out. In total, 128 fermented foods were purchased from department stores and traditional markets in Korea. The samples comprised 55 lactic acid–fermented vegetable (kimchi), 53 fermented soybean paste (doenjang, gochujang, cheonggukjang, and ssamjang), 20 fermented fish products (jeotgal), 18 yoghurt, 37 bread, 12 cheese, and 23 vinegar samples. The level of EC in fermented soybean pastes ranged from not detectable to 240.2 ng g^?1. EC was not detected in kimchi, jeotgals, yoghurt, and cheese. Bread and vinegar contained EC up to 3.70 and 16.79 ng g^?1, respectively. The estimated daily intake and chronic daily intake of EC were 1.98 ng kg body weight (bw)^?1 day^?1, 1.50 ng kg bw^?1 day^?1, respectively. Excess cancer risk and margin of exposure of EC were 7.5 × 10^?8 and 151,515, respectively.     

Nutrient and sensory qualities of extruded malted or unmalted millet/soybean mixture

Complementary blends were prepared from mixtures of malted or unmalted millet and soybean. The malted or raw millet was fortified with malted or raw soybean grain mixture at two ratios: 50% (an equal amount to the millet) or 30%. The mixture was extruded in a single screw 600 Jr. insta pro-extruder. The extruded mixtures were analysed for nutrient and sensory qualities. The crude protein was least (18.3%) in unmalted mixtures of 70% millet and 30% soybean (RM70RS30). A significantly (P<0.05) higher protein value of 28.9% was observed in malted mixtures (MM50MS50). Extruded combinations of 50% raw millet and 50% raw soybean (RM50RS50) had a significantly (P<0.05) higher gross energy value (1819 kJ/100 g) than other mixtures. There were significant differences between the calcium, phosphorus, magnesium, sodium and zinc levels of the mixtures. The mixtures were relatively high in minerals. Lysine and methionine content in the mixtures varied respectively, from 4.39 g/100 g protein in RM70RS30 to 5.30 g/100 g protein in MM50MS50 and 1.28 g/100 g protein in RM5ORS5O to 12 17 g/100 g⁻¹ protein in MM70MS30. The sensory evaluation showed that taste, flavour and texture increased with a greater proportion of malted ingredients, but the colour became unacceptable. While increase in the level of soybean improved the chemical values, mixtures with less soybean were more acceptable.     

Formation of heterocyclic amines during cooking of duck meat

Heterocyclic amines (HAs) are an important class of food mutagens and carcinogens produced in meat cooked at high temperature. In the present study, the effects of various cooking methods: boiling, microwave cooking, charcoal-grilling, roasting, deep-frying and pan-frying on the formation of HAs in duck breast were studied. The various HAs formed during cooking were isolated by solid-phase extraction and analysed by HPLC. Results showed that both the varieties and contents of HAs and the cooking loss of duck breast increase along with increasing cooking temperature and time. Pan-fried duck breasts contained the highest amount of total HAs, followed by charcoal-grilling, deep-frying, roasting, microwave cooking and boiling. 9H-pyrido[3,4-b]indole (norharman) and 1-methyl-9H-pyrido[3,4-b]indole (harman) were detected in all of the cooked duck meat, with levels in the range of 0.1–33?ng?g^?1. 2-Amino-1-methyl-6-phenylimidazo[4,5-f]pyridine (PhIP) was formed easily in duck meat cooked by pan-frying and charcoal-grilling in the range of 0.9–17.8?ng?g^?1. 2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) was identified in duck meat cooked by charcoal-grilling and pan-frying, in the range of 0.4–4.2?ng?g^?1. 2-Amino-3,8-dimethyl-imidazo[4,5-f]quinoxaline (MeIQx) was detected in amounts below 4.5?ng?g^?1 in duck meat cooked by charcoal-grilling, roasting, deep-frying and pan-frying. The other HAs were detected in amounts below 10?ng?g^?1. Colour development increased with cooking temperature, but no correlation with HAs’ content was observed.     

Furan in commercial baby foods from the Spanish market: estimation of daily intake and risk assessment

ABSTRACT

The occurrence of furan in commercial baby food samples from the Spanish market was evaluated using an automated headspace solid-phase microextraction method coupled to gas chromatography-mass spectrometry (HS-SPME-GC-MS). A total of 76 baby food samples including infant formula, baby cereals, fruit in cans and/or jars, vegetables, meat, and fish, were surveyed for furan content. The lowest concentration of this compound was found in infant formula (<0.02–0.33 ng ml^?1), and cereal-based food (0.15–2.1 ng g^?1) while baby food containing fish showed the highest concentrations (19–84 ng g^?1). Following recommendation of the European Food Safety Authority (EFSA), the effect on furan content was evaluated of consumer home preparation of foods, heating and handling. Furan concentrations were reduced by up to 35% when samples were heated in a dish using microwave oven and by up to 53% when a hot water bath was used. Finally, we estimated the furan intake from baby food consumption (0.002–1.18 µg kg^?1 body weight day^?1) and we calculated the margin of exposure (MOE) from samples as purchased and also after home preparation of the food. For infant formula and cereal baby foods, the MOEs (26,278–412,776) indicated no infant health concern or priority, while for meat and fish-based baby foods the values pointed to a potential public health risk, even considering the furan losses during preparation at home.     

Effect of extraction method and ripening stage on banana peel pigments

Carotenoids are one of the most widespread pigments in nature and can be used as health‐promoting natural food colorants. Banana peel, which is a by‐product of banana processing, contains a range of bioactive compounds including carotenoids. There is no published research on the extraction of food‐grade carotenoids from banana peels. This study evaluated the change in the banana peel carotenoid content over its ripening stages and determined the best possible solvent to extract carotenoid for food applications. The solvents permitted under Food Standard Australia New Zealand were used in the study. Ripeness stage 5 contained the highest content of total carotene at 1.86 μg g^?1 of banana peel. From one gram of banana peel, 0.57 μg of xanthophyll and 0.84 μg of beta‐carotene were extracted from ripening stage 5 with a solvent combination of hexane–diethyl ether–acetone and hexane–diethyl ether, respectively.     

Potential of Bambara Groundnut (Vigna subterranea (L.) Verdc) Milk as a Probiotic Beverage—A Review

Bambara groundnut (Vigna subterraenea (L.) verdc) (BGN) is a legume; its origin have been traced back to Africa, and it is the third important legume; however, it is one of the neglected crops. It is highly nutritious, and has been termed a complete food. Its seed consist of 49%–63.5% carbohydrate, 15%–25% protein, 4.5%–7.4% fat, 5.2%–6.4% fiber, 3.2%–4.4% ash and 2% mineral compared to whole fresh cow milk 88% moisture, 4.8% carbohydrate, 3.2% proteins, 3.4% fat, 0.7% ash, and 0.01% cholesterol. Its chemical composition is comparable to that of soy bean. Furthermore, BGN has been reported to be a potential crop, owing to its nutritional composition, functional properties, antioxidant potential, and a drought resistant crop. Bambara groundnut milk (BGNM) had been rated higher in acceptability than milk from other legumes like soybean and cowpea. Probiotics have been defined as live microorganisms which when administered in adequate amount confer a health benefit on the host. These benefits have been reported to be therapeutic, suppressing the growth and activity in conditions like infectious diarrhea, irritable bowel syndrome, and inflammatory bowel disease. The nutritional profile of BGNM is high enough to sustain the growth of probiotics. BGNs are normally boiled and salted, eaten as a relish or roasted, and eaten as a snack. Hence, BGNM can also be fermented with lactic acid bacteria to make a probiotic beverage that not only increase the economic value of the nutritious legume but also help in addressing malnutrition.     

Cassava traits and end-user preference: Relating traits to consumer liking,sensory perception,and genetics

Breeding efforts have focused on improving agronomic traits of the cassava plant however little research has been done to enhance the crop palatability. This review investigates the links between cassava traits and end-user preference in relation with sensory characteristics. The main trait is starch and its composition related to the textural properties of the food. Pectin degradation during cooking resulted in increased mealiness. Nutritional components such as carotenoids made the cassava yellow but also altered sweetness and softness; however, yellow cassava was more appreciated by consumers than traditional (white) varieties. Components formed during processing such as organic acids gave fermented cassava products an acidic taste that was appreciated but the fermented smell was not always liked. Anti-nutritional compounds such as cyanogenic glucosides were mostly related to bitter taste. Post-harvest Physiological Deterioration (PPD) affected the overall sensory characteristics and acceptability. Genes responsible for some of these traits were also investigated. Diversity in cassava food products can provide a challenge to identifying acceptance criteria. Socio-economic factors such as gender may also be critical. This review leads to questions in relation to the adaptation of cassava breeding to meet consumer needs and preference in order to maximize income, health and food security.     

Compositional variation in β‐carotene content,carbohydrate and antioxidant enzymes in selected banana cultivars

Banana is one of the most important tropical fruits, and India ranks first in its world production. Banana cultivars rich in provitamin A carotenoids may offer a potential food source for alleviating vitamin A deficiency, particularly in developing countries. With a view to exploit banana peels as a source of valuable components, in the present study, proximate composition, carotenoids, beta‐carotene and some anti‐oxidative enzymes as well as carbohydrate content of selected Indian banana varieties were determined. Karpooravalli cultivar of banana showed the maximum accumulation of carotenoid content in the non‐edible (68 μg g^?1 d.w.) portion of banana, while being the second highest in beta‐carotene content (143.12 μg per 100 g). However, Red Banana ranked highest in total carotenoid contents for pulp (4 μg g^?1 d.w.) and beta‐carotene was estimated to be the highest in the case of peels (241.91 μg per 100 g) and in pulp (117.2 μg per 100 g). Karpooravalli cultivar of banana is also rich in carbohydrate content in terms of total starch (1786.0 μg g^?1 d.w. in peels and 544.85 μg g^?1 d.w. in pulp) and sugars (53.53 μg g^?1 d.w. in peels and 39.05 μg g^?1 d.w. in pulp). The catalase enzyme activity in these peels ranged from 5.66 to 35.57 nmol min^?1 mg^?1 proteins and was found at a higher level in cultivar Poovan, while the ascorbate peroxidase showed the range of 2.25 to 6.22 μmol min^?1 mg^?1 proteins. The peels of cultivars Red Banana and Karpooravalli are rich source of bioactive compounds, such as carotenoids (beta‐carotene), anti‐oxidative enzymes and carbohydrate contents.     

Development and nutritional quality evaluation of weaning foods based on malted, popped and roller dried wheat and chickpea

Optimal conditions for malting wheat and chickpea for preparation of weaning foods were standardized and malted flours from 48 h germinated wheat and 24 h germinated chickpea were blended to prepare malted weaning food. Wheat was dry-heat-parboiled (bulgurized), popped in hot sand and blended with popped chickpea flour to prepare popped weaning food. Mildly toasted and debranned wheat and dehusked chickpea flours were mixed and the blend was roller-dried for preparation of roller dried weaning food. the formulations had 60% wheat, 30% chickpea, 5% skim milk powder and 5% sucrose and contained about 16% protein. the cooked paste viscosity (dietary bulk) of malted food, popped food with malt, and roller dried food with malt was significantly lower than popped and roller dried foods at all comparable slurry concentrations. the energy density of malted and malt-added food slurries at spoon feeding consistency was 4.2 KJg⁻¹. PER (2.91), biological value (88.3) and true digestibility (87.5) values of malted food were higher than that of the other formulations.     

Nutraceuticals in gummy candies form prepared from lacto‐fermented lupine protein concentrates,as high‐quality protein source,incorporated with Citrus paradise L. essential oil and xylitol

As an addition to a normal diet, the use of food enriched with functional compounds has become increasingly popular. Alternatively, functional ingredients can be incorporated into nutraceuticals in gummy candies (GC) form. This study aimed to adapt lacto‐fermented lupine protein concentrates, as high‐quality protein and genistein sources, with Citrus paradise essential oil (EO), an antimicrobial and bean taste masking agent, and xylitol, a low glycaemic index sweetener, for preparation of nutraceuticals in GC form. For GC preparation, submerged fermentation with Lactobacillus sakei lupine variety Vilniai protein concentrate was selected, which had the highest protein content (90.11%), protein digestibility (89.94%) and genistein content (30.93 μg g^?1) and the lowest trypsin inhibitors activity (19.40%), among the concentrates assessed. GC formulated with xylitol, ascorbic acid, grapefruit EO (up to 0.2%) and lupine protein concentrate (up to 13.0%) allowed obtaining products that exhibited a good texture, high overall acceptability and contained desirable functional compounds.