Hermetic storage as an alternative for controlling Callosobruchus maculatus (Coleoptera: Chrysomelidae) and preserving the quality of cowpeas

The aim of this study was to assess the use of modified atmosphere through hermetic storage in polyethylene silo bags and polyethylene terephthalate (PET) bottles as a technique to control Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae: Bruchinae) and to preserve the quality of cowpeas during storage. Cowpea grains were stored in polyethylene silo bags, polyethylene terephthalate (PET) bottles and glass recipients (control) for 30, 60, 90 and 120 days. Each treatment was replicated four times. After each storage period, we assessed the insect infestation percentage and measured the moisture content, the bulk density, the germination percentage and the electrical conductivity of the grains. The percentage of infestation by C. maculatus for cowpeas stored in silo bags and PET bottles was low and did not exceed 4% during the entire storage period. In contrast, the percentage of infestation by C. maculatus increased in untreated cowpeas over the storage period. The moisture content, bulk density, germination percentage and electrical conductivity of the cowpeas were preserved in both hermetic storage systems that were tested for 120 days. To conclude, the hermetic storage of cowpeas using silo bags and PET bottles can efficiently control C. maculatus and preserve the quality of cowpeas for at least 120 days of storage.     

Impact of moisture content and maize weevils on maize quality during hermetic and non-hermetic storage

The objective of this study was to determine the impact of moisture content and Sitophilus zeamais Motschulsky on maize quality during hermetic and non-hermetic storage conditions. Commercial Channel 211-97 hybrid maize kernels were conditioned to 14, 16, 18, and 20% moisture content (wet basis), and then three replications of 300 g of maize grain were stored in glass jars or triple Ziploc^® slider 66-μm(2.6-mil) polyethylene bags at four conditions: hermetic with weevils, hermetic no-weevils, non-hermetic with weevils, non-hermetic no-weevils. All jars and bags were stored in an environmental chamber at 27 °C and 70% relative humidity for either 30 or 60 d. At the end of each storage period, jars and bags were assessed for visual mold growth, mycotoxin levels, gas concentrations, pH level, the numbers of live and dead S. zeamais, and maize moisture content. The maize stored in non-hermetic conditions with weevils at 18 and 20% exhibited high levels of mold growth and aflatoxin contamination (>150 ppb). Conversely, very little mold growth was observed in maize stored in hermetic, and no aflatoxins were detected in any moisture level. CO₂ increased and O₂ gradually decreased as storage time increased for maize stored in hermetic conditions (with or without weevils) in all moisture level. No significant difference in pH was observed in any storage conditions (P < 0.05). Total mortality (100%) of S. zeamais was observed in all hermetically stored samples at the end of 60 days storage. Moisture content for hermetically stored maize was relatively constant. A positive correlation between moisture content and storage time was observed for maize stored in non-hermetic with weevils (r = 0.96, P < 0.05). The results indicate that moisture content and the number of S. zeamais weevils plays a significant role in maize storage, both under hermetic and non-hermetic conditions.     

Efficacy of metal silos and hermetic bags against stored-maize insect pests under simulated smallholder farmer conditions

Pesticide-free hermetic grain storage is an environmentally-benign alternative to synthetic pesticides, currently being used in many countries. However, in some African countries knowledge gaps exist on the effectiveness of hermetic maize storage, particularly where the Larger Grain Borer (LGB), Prostephanus truncatus occurs. Trials simulating African smallholder farmer conditions were conducted at two sites in contrasting agro-ecological zones in Zimbabwe for up to 12 months during the 2013/14 storage season. There were two hermetic treatments: metal silos and hermetic bags; and two non-hermetic treatments: a registered synthetic pesticide and untreated control, in polypropylene bags. Two modes of infestation: natural and combined (natural plus artificial) were used as factors. Treatments were arranged in a completely randomised design and stored in ordinary rooms. Hermetic treatments were significantly superior (P < 0.001) to non-hermetic treatments in preserving germination, controlling insect population development, suppressing maize grain damage, controlling grain dust production and consequently limiting weight loss during storage. Hermetic bags were more effective than non-hermetic treatments in reducing storage losses despite the plastic liners having multiple insect-induced perforations of more than 300 holes per plastic liner at termination. However, there were no significant differences between metal silos and hermetic bags regardless of the mode of infestation. There was strong correlation between total insect population per kg and: percentage grain damage, percentage weight loss, and grain dust which indicate the importance of controlling insect pest development during storage to reduce losses. Results show that hermetic storage can be an effective pesticide-free alternative to synthetic pesticides in reducing grain storage losses under smallholder farming conditions, even where LGB occurs.     

Storage of WDGS under hermetic and non-hermetic conditions: Effect on sensory properties,microorganisms, mycotoxins and nutritional value

WDGS production in Argentina substantially increased in the last 5 years and become a frequent constituent for feed diets. However, due to the extremely high moisture content of the product, quality deterioration occurs in a few days under aerobic conditions; which creates a series of logistical problems for expanding the use of WDGS far away from production plants. Silo bag technology, a flexible hermetic storage system extensively used in Argentina, could be a good alternative for extending the storage time of WDGS. Thus, the main goal of this research was to compare the effect of hermetic and non-hermetic storage of WDGS using several quality parameters. Samples of WDGS from three different ethanol production plants were stored in hermetic (small scale silo bags) and non-hermetic conditions at 16 °C (60 days hermetic and 20 days no-hermetic). WDGS quality evolution was evaluated through sensory, microbiological, biochemical, nutritional and toxicological parameters. Results indicated that in the non-hermetic treatment, even though no evidence of nutritional quality losses was found in the mass of WDGS, spoilage developed at the surface after 10 day of storage. Additionally, evidence of mycotoxin formation was found (DON) during aerobic storage, implying that mycotoxin tests should be routinely implemented. On the contrary, hermetic storage in silo bag allowed a proper ensiling process, extending the shelf life of the product and preventing mycotoxins development for at least 60 days.     

Comparative performance of five hermetic bag brands during on-farm smallholder cowpea (Vigna unguiculata L.Walp) storage

Cowpea (Vigna unguiculata L. Walp) grain is an important source of protein for smallholder farmers in developing countries. However, cowpea grain is highly susceptible to bruchid attack, resulting in high quantitative and qualitative postharvest losses (PHLs). We evaluated the performance of five different hermetic bag brands for cowpea grain storage in two contrasting agro-ecological zones of Zimbabwe (Guruve and Mbire districts) for an 8-month storage period during the 2017/18 and 2018/19 storage seasons. The hermetic bag treatments evaluated included: GrainPro Super Grain bags (SGB) IVR™; PICS bags; AgroZ® Ordinary bags; AgroZ® Plus bags; ZeroFly® hermetic bags. These were compared to untreated grain in a polypropylene bag (negative control) and Actellic Gold Dust® (positive chemical control). All treatments were housed in farmers’ stores and were subjected to natural insect infestation. Hermetic bag treatments were significantly superior (p < 0.001) to non-hermetic storage in limiting grain damage, weight loss and insect population development during storage. However, rodent control is recommended, as rodent attack rendered some hermetic bags less effective. Actellic Gold Dust® was as effective as the hermetic bags. Callosobruchus rhodesianus (Pic.) populations increased within eight weeks of storage commencement, causing high damage and losses in both quality and quantity, with highest losses recorded in the untreated control. Cowpea grain stored in Mbire district sustained significantly higher insect population and damage than Guruve district which is ascribed to differences in environmental conditions. The parasitic wasp, Dinarmus basalis (Rondani) was suppressed by Actellic Gold Dust® and all hermetic treatments. All the hermetic bag brands tested are recommended for smallholder farmer use in reducing PHLs while enhancing environmental and worker safety, and food and nutrition security.     

Pest control treatments with phosphine and controlled atmospheres in silo bags with different airtightness conditions

The silo bag technology has been extensively used in Argentina for storing grains (e.g. wheat, corn, barley, sunflower and soybean among others) since the mid-1990s. Silo bag are widely considered a hermetic storage system in which PH₃ fumigation is frequently implemented for pest control. However, there is insufficient information regarding the potential airtightness of silo bags and how it could affect the performance of fumigation and controlled atmosphere treatments. In this study, a pressure decay test (PDT) was implemented to characterize airtightness level of silo bags set up following various procedures. PH₃ fumigation treatments with different dosages and hermeticity levels were conducted, and fumigant concentration was monitored. Controlled atmosphere treatments with carbon dioxide were also implemented in silo bags with different hermeticity levels. Results showed that less than half of the bags tested in the field had a PDT indicated for fumigation (90 s), and that when a bag without thermo sealing was used for fumigation, this treatment failed. However, it was demonstrated that with simple and inexpensive practices silo bags can achieve high enough airtightness conditions to implement successful PH₃ fumigation (5 days above 200 ppm with a dosage of 1 g of PH₃/m³) and even controlled atmosphere treatments (more than 18 days with CO₂ concentration above 70%). This study shows that silo bags could be used as a cost competitive hermetic storage technology for performing controlled atmosphere treatments.     

Comparative effects of hermetic and traditional storage devices on maize grain: Mycotoxin development,insect infestation and grain quality

A large-scale study was conducted to assess which of the five most accessible hermetic storage devices on the Kenyan market fulfill the needs of smallholder farmers by positively impacting three major areas of concern: insect infestation, grain quality, and mycotoxin (aflatoxin and fumonisin) contamination. Efficacy of two hermetic silos (plastic and metal) and three hermetic bags (PICS, GrainPro's GrainSafe™, and Super Grain) was directly compared to current maize storage in polypropylene (PP) bags under local environmental conditions using representative storage volumes during a 6-month storage period. Impact of maize grain stored at typical (∼15%) and recommended (<13.5%) moisture levels and potential efficacy losses through frequent interruption of the underlying hermetic principals was assessed. Hermetic storage significantly reduced the increase in aflatoxin compared to PP bags regardless of the moisture level of the grain. An <5% per month aflatoxin increase was achieved by three of the five devices tested: Metal silo, PICS and GrainSafe™ bag. A strong correlation between grain moisture, storage time and aflatoxin development was found in PP bags, but not in any of the hermetic devices. The same result was not obtained for fumonisin development in stored maize. The rate of Fumonisin increase was similar in all tested devices, including the polypropylene bags, and conditions. The periodic opening of the hermetic devices had no significant effect on the efficacy of the hermetic devices but the repeated disturbance of the PP bags led to a significant increase in aflatoxin levels. The maize weevil Sitophilus spp. was most commonly found with a total incidence of 72%. Grain storage under hermetic conditions reduced insect infestation, grain weight loss and discoloration. However, maize storage above recommended moisture levels led to a distinct odor development in all hermetic devices but not the PP bags. Hence, proper grain drying is a prerequisite for maize storage in airtight conditions.     

Physical factors in the hermetic SuperGrainBag® and effect on the larger grain borer [Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae)] and aflatoxin production by Aspergillus flavus during the storage of ‘Obatanpa’ maize (Zea mays L.) variety

Controlling invasive pests and aflatoxin production by moulds in stored grains is a global challenge to food security and public health, particularly in Africa. Food storage systems are designed to provide constraints to spoilage organisms by presenting mechanical barrier or unfavourable atmospheric conditions to their growth and productivity. This study examined the physical factors generated in hermetic SuperGrainBags^® during the storage of ‘Obatanpa’ variety of maize (Zea mays L.) and the effect on growth of the larger grain borer Prostephanus truncatus (Horn) and aflatoxin contamination by Aspergillus flavus. Cultured P. truncatus or A. flavus was introduced into 1.5 kg of the dried maize and stored in either hermetic SuperGrainBags^® or non-hermetic polypropylene bags. The hermetic conditions elicited an increase in the interstitial temperature (ca. 27 °C) but a decrease in the relative humidity (<70%), oxygen concentration (<6.4%) and the grain moisture content (<13.7%), the combined effects of which inhibited growth of the insects and aflatoxin production by the moulds. Total mortality of P. truncatus occurred after 52 d of storage in the SuperGrainBags^® while aflatoxins concentration remained within safe limits for human consumption. In contrast, there was proliferous growth of P. truncatus and significant increase in aflatoxin concentration to lethargic levels within the polypropylene bags where temperature, relative humidity and grain moisture increased significantly. Accordingly, grain damage and weight loss percentages were significantly high in the polypropylene bags while that in the SuperGrainBags^® were negligible. Altogether, the SuperGrainBags^® better preserved the maize grain quality and safeguarded it against health risks than the polypropylene bags.     

Effectiveness of grain storage facilities and protectants in controlling stored-maize insect pests in a climate-risk prone area of Shire Valley,Southern Malawi

Shire Valley is one of Malawi's most vulnerable areas to climate change (CC). In addition to other impacts, CC is expected to affect storage insect pest status, and the efficacy of grain storage facilities and protectants. On-farm grain storage trials were therefore conducted in Shire Valley to assess the performance of storage facilities and grain protectants against storage insect pests. Eight smallholder farmers hosted the trials in Thyolo and Chikwawa districts. Seven grain storage treatments were evaluated for 32 weeks during two storage seasons: Neem leaf powder (NM), Actellic Super dust (ASD), ZeroFly^® bag (ZFB), Purdue Improved Crop Storage bag (PICS), Super Grain Bag (SGB), hermetic metal silo (MS) and untreated grain in a polypropylene bag (PP). Insect pest populations and grain damage increased with storage duration and differed significantly between treatments (p < 0.05). Grain stored in hermetic bags (PICS, SGB) sustained significantly lower (p < 0.05) insect damage and weight loss compared to other treatments across sites and seasons. The hermetic bags also outperformed the other treatments in suppressing insect numbers. However, germination rates of undamaged grains stored in the hermetic storage facilities (MS, PICS, SGB) for 40 weeks were extremely low (<15%) compared to that of undamaged grains from NM treatment (53–58%) and the other treatments (>75%) at both sites. The hermetic MS, ZFB bags, ASD and NM treatments did not effectively protect grain from insect damage. High in-store mean temperature (35.6 °C) and high initial grain moisture content (13.7%) may have negatively affected efficacy of some treatments and seed germination. Tribolium castaneum survival in the MS requires further investigation. The hermetic storage bags (PICS, SGB) can be recommended for long-term maize grain storage (≥32 weeks) by smallholder farmers in Shire Valley and other similar climate change-prone areas in sub-Saharan Africa.     

Triple bag hermetic technology for post-harvest preservation of Bambara groundnut (Vigna subterranea (L.) Verdc.)

Experiments were conducted to evaluate the performance of hermetic triple bagging using Purdue Improved Crop Storage (PICS) bags for storage of Bambara groundnut (Vigna subterranea (L.) Verdc.). One set of experiments used grain heavily infested by Callosobruchus maculatus (F.) while a second set began with a low level infestation. Each experiment consisted of V. subterranea grain kept in four replicate 50 kg PICS bags or two replicate woven bags as controls. Two to five days after the beginning of the experiments, oxygen level inside the bags averaged about 21% (v/v) in the controls but decreased significantly in PICS bags, reaching 10% (v/v) with the heavily infested grain but falling only slightly in the lightly infested grain. After 7 months of storage, (i) the number of C. maculatus adults found between and within the grains, (ii) the 100 seed weight, (iii) the number of seed with holes, and (iv) the percentage germination of grain stored in PICS bags did not differ from what had been measured on the day that the experiment was set up. In woven bags, by contrast, there was a massive increase in C. maculatus numbers with means of 309 and 251 adults per 500 g in heavily and lightly infested grain, respectively. Grain weight losses in the woven bag controls ranged from 8 to 19% and the percentage of C. maculatus emergence holes per 100 seed increased from 51 to 135%.     

Desiccant drying prior to hermetic storage extends viability and reduces bruchid (Callosobruchus chinensis L.) infestation of mung bean (Vigna radiata (L.) R. Wilczek) seeds

Seeds of mung bean (Vigna radiata (L.) R. Wilczek) are subject to loss of viability due to aging and damage from pulse beetles (or bruchids; Callosobruchus spp.) infestation during storage. We investigated whether seed drying using desiccants and hermetic packaging would prevent or ameliorate these consequences of storage. Sun-dried mung bean seeds at a moisture content of 10% were subjected to further drying for 72 h using five different desiccants: Drying Beads® (a zeolite-based desiccant), silica gel, sodium aluminum silicate, activated alumina, and cow-dung ash (a traditional desiccant). Seeds were subsequently stored in hermetic plastic containers in the presence of these desiccants under ambient conditions along with sun-dried seeds stored in cloth bags or in hermetic containers. In addition, parallel samples of each treatment were inoculated with one pair of bruchid beetles (C. chinensis L.) and stored under the same conditions. The seed drying treatments did not affect initial seed quality (germination percentage and seedling vigor) significantly. After storage for 9 months at ambient temperatures, seeds dried using Drying Beads, silica gel, sodium aluminum silicate and activated alumina had higher germination percentages, seedling vigor indices and soil emergence, and lower electrical conductivity (leakage upon imbibition) and fungal infestation compared to other conditions. In addition, the mung bean seeds inoculated with bruchids and stored with these effective desiccants had less damage, oviposition, and insect respiratory activity in the hermetic containers and maintained higher seed germination and seedling vigor after six months of storage compared to other treatments and controls. The results demonstrate the superior ability of desiccants to quickly and safely dry seeds prior to and during storage and the benefits of such drying and hermetic storage conditions for preventing seed deterioration and insect damage during storage.     

Storage of green coffee in hermetic packaging injected with CO2

The objective of this study was to evaluate the physical, chemical, and sensory qualities of green coffee beans (Coffea arabica L.) during storage in different types of packaging. Coffee was stored from October 2008 to September 2009 in a warehouse of the Agriculture Society Ltda. (SAAG) in Santana da Vargem, southern Minas Gerais State, Brazil. The treatments in the factorial design consisted of two types of packaging (hermetic big bags with the injection of up to 60% CO₂ in a controlled atmosphere; similar bags but without the injection of CO₂ in a modified atmosphere) and three sampling positions in the bags (high, medium, and low). At 3-month intervals during a 12-month period, grains were analyzed to determine their water content, color, electrical conductivity, potassium lixiviation, and content of sugars. Sensory analysis was also conducted at these sampling times. The storage of green coffee beans in hermetic big bags on a commercial scale under modified and controlled atmospheric conditions is viable over a 12-month period. The coffee packed in big bags maintained its quality and exhibited an intensification of the green coloration of the grains during storage. Sensory analysis of coffee beans stored in a controlled atmosphere showed that the medium sampling position yielded the best ratings. The results of this analysis demonstrated that this storage technique can potentially increase the effectiveness of methods used to preserve the sensory quality of coffee beans.     

Sorghum seed storage in Purdue Improved Crop Storage (PICS) bags and improvised containers

Seed storage is a major challenge for smallholder farmers in developing nations. Purdue Improved Crop Storage (PICS) bags effectively control the postharvest insect pests of cowpea and other crops. Farmers, encouraged by this success, have begun to expand the use of PICS bags for storing other crops. Little is known about how sorghum seed, one of these important crops, fares when stored under hermetic conditions. Accordingly, we stored sorghum seed for six months in either airtight containers (PICS bags or sealed plastic bottles) or open ones (woven polypropylene bags and open plastic bottles). Overall, sorghum seed stored in PICS bags and in sealed plastic bottles maintained its initial moisture level, germination rate and seed weight. Porous polypropylene bags and open plastic bottles lost moisture over six months. We conclude that sorghum seed can be safely stored in hermetic containers without any loss of quality for extended periods of time.     

Evaluations of the new deltamethrin-treated all-in-one hermetic bag for the control of the Khapra beetle,Trogoderma granarium (Everts)

The khapra beetle, Trogoderma granarium (Everts), is a highly destructive stored product insect that presents a significant threat to stored bagged grain. Hermetic packaging is designed to maintain the quality and safety of stored grain, while continually protecting the grain from insect infestations during storage. The objective of this research was to evaluate a prototype deltamethrin, all-in-one treated hermetic bag on contact efficacy, larval mobility, and the control of T. granarium in artificially infested wheat. The insecticidal activity of the deltamethrin-treated packaging was tested against larvae and adults of T. granarium through contact bioassays. There was a significant reduction in responsive adults after 5 day and >86% of larvae were unresponsive after 9 d of exposure on the treated bag. Trogoderma granarium movement toward a food bait was <10% after a 24 h exposure on the treated bag compared to ~45% of larvae on untreated bag. Lots of 15 kg of wheat were artificially infested with 100 T. granarium larvae and placed inside treated and untreated storage bags, sealed, and stored in a semi-field warehouse, and observed after 2, 6, and 8-weeks for T. granarium survival and grain quality attributes. The weight and number of insect damaged kernels was lower across all storage intervals for grain held in the treated bags, as compared with control bags. Live adult T. granarium were observed at 2-weeks in treated and untreated bags, but there were no live adults observed after 8-weeks of storage in both bags. The new prototype hermetic bags maintained positive grain qualities, however more information on the hermetic parameters are needed to understand how some individuals survived.     

Field effectiveness of improved hermetic storage technologies on maize grain quality in Central Mexico

Maize is the main crop cultivated by small scale farmers (SSF) in Mexico, and its production represents an important goal for local food security. However, SSF very often face severe post-harvest losses in maize, mainly because of insect pests and the lack of suitable storage technology. This study was conducted to compare the field effectiveness in terms of maize quality under on-field conditions of SSF of two improved hermetic storage technologies with the traditional storage. The field experiment was performed on-farm in the highlands of Central Mexico. Maize grains were stored in three storage devices: hermetic plastic bag (sBag), hermetic plastic silo (Bioxilo), or traditional polypropylene sacks. The study considered the quantitative evaluation of storage, physical, nutritional, and industrial qualities of maize after 4, 8 and 12 months of storage. Environmental conditions of storage such as oxygen levels, temperature and humidity were monitored. After 1 year of field storage, compared with traditional sacks, sBag and Bioxilo showed a significant lower losses in storage due to insects. When controlled infested maize by artificial insect infestation methods was used, the improved technologies were also effective in reducing grain losses. Furthermore, both sBag and Bioxilo prevented grain quality detriment in terms of physical, nutritional and industrial (for tortilla and seed) properties (p < 0.01). Additionally, compared with traditional storage, sBag and Bioxilo showed efficient control of the hermetic conditions in terms of oxygen, temperature and humidity, but no significant differences were detected between the two improved technologies. In summary, under on-farm conditions, the successful preservation of maize grain quality was achieved by the improved sBag and Bioxilo in the highlands of Mexico. In the future, positive effects of this storage system will need to be validated under tropical conditions.     

Comparative utility of hermetic and conventional grain storage bags for smallholder farmers: a meta-analysis

Postharvest management is critical to attaining household food, nutrition, and income security. Hermetic grain storage bags offer an effective pesticide-free way to protect stored grain against fungal and insect infestation. We evaluated articles indexed in the Web of Science that included experiments comparing the storage efficacy of conventional and hermetic storage bags based on grain germination rate, insect infestation, physical damage, mycotoxin contamination, and changes in weight and moisture content. Compared with grain stored in hermetic bags, grain stored in conventional bags lost 3.6-fold more seed viability, contained 42-fold more insects, had 11-fold more physical damage, and lost 23-fold more grain weight, while grain moisture levels were similar for both hermetic and conventional storage bags. Mycotoxin contamination levels were not as frequently assessed. Levels could be low in grain stored in both types of bags, or levels could be low in hermetic bags and significantly higher in conventional bags. The improved properties of grain stored in hermetic bags can increase food security and household income by providing safe storage options for maintaining seed germinability, and for consumption and/or sale when food supplies are high, or when prices are low. Hermetic bags are economically feasible for use by subsistence farmers in Sub-Saharan Africa for grain for household consumption and for carrying-over seed for planting in the next season. Additional studies are needed to verify the mycotoxin contamination results and to determine if there are differences in functional food characteristics, e.g. flavor and cooking properties, that have not been as comprehensively studied. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.     

The use of modified atmospheres to control Sitophilus zeamais and Sitophilus oryzae on stored rice in Portugal

This study reports the efficacy of using CO₂ against Sitophilus zeamais and Sitophilus oryzae as an alternative treatment to fumigation for rice stored in a rice mill in Portugal. The trials were conducted in a silo containing 40 tonnes of polished rice and in four hermetic big bags of 1 tonne capacity; two with paddy and two with polished rice. The composition of the atmosphere ranged from 90 to 95% CO₂ and 0.7–2.1% O₂. Three trials were carried out at different temperatures and treatment times; stored rice in the silo at 29.6 ± 0.1 °C for 26 days (first trial), at 34.1 ± 0.2 °C for 10 days (second trial), and in big bags at 22 °C for 26 days (third trial).To evaluate the efficacy of each treatment, metal cages with 16 g of infested rice where placed at bottom, middle, top and surface of the polished rice in the silo. Four replications of each type of infested rice containing one-week-old S. zeamais adults, or eggs of S. zeamais or S. oryzae, were incubated in the laboratory, at the same temperature as in the silo, to serve as a control.In all modified atmosphere treatments adults of S. zeamais, and eggs of both S. oryzae and S. zeamais, showed mortality close to 100% and no F1 emergence was recorded in any treatment sample. This was the first time that a Portuguese rice mill used modified atmospheres.     

Storage of mung bean (Vigna radiata [L.] Wilczek) and pigeonpea grains (Cajanus cajan [L.] Millsp) in hermetic triple-layer bags stops losses caused by Callosobruchus maculatus (F.) (Coleoptera: Bruchidae)

Dry mung bean and pigeonpea grains that had sustained some insect damage but fumigated before the start of the experiment were stored in triple-layer hermetic bags (Purdue Improved Crop Storage (PICS™ bags) or woven polypropylene (PP) bags for 6 months. Some of the bags were artificially infested with cowpea bruchid Callosobruchus maculatus (F.) (PICS1, PP1) while others were not (PICS0, PP0). In an additional trial, PP bags containing the grains were treated with Actellic Super^® dust before being artificially infested (PP1Ac). Moisture content, live adult C. maculatus count, grain damage, weight loss, and seed germination were determined on a monthly basis. At six months, moisture contents of grain stored in PICS and PP bags remained below 12%. Storage in PICS bags halted multiplication of C. maculatus, and the initial damage level and weight of grains did not change. Conversely, in the PP bags, C. maculatus populations increased massively and seed damage reached 71.8 ± 1.9%, 76.9 ± 0.4%, and 60.3 ± 0.6% corresponding to weight losses of 14.5 ± 0.1%, 16.5 ± 0.2% and 12.5 ± 0.1% in PP0, PP1 and PP1Ac, respectively, in mung beans. With the pigeonpeas, seed damage reached 55.1 ± 0.6%, 95.7 ± 0.4% and 75.8 ± 0.9%, corresponding to weight losses of 13.0% ± 0.3%, 26.2 ± 0.2% and 13.5 ± 0.1%, in PP0, PP1 and PP1Ac, respectively. PICS bags are an effective tool for preserving mung beans and pigeonpeas against C. maculatus attack, and their performance is superior to that of Actellic Super^® dust.     

Efficacy of sensor assisted vacuum hermetic storage against chemical fumigated wheat

Alternatives to chemical fumigation, are being explored as the popular fumigants like methyl bromide and phosphine are being phased out for their ozone depleting nature and insect resistance, respectively. Vacuum hermetic storage has potential for storage of agricultural durable commodities without fumigants and can eliminate 99% of insect infestation. In present research, the vacuum hermetic storage was tested in field with assistance of sensors and compared with the conventional phosphine fumigated storage by grain quality assessment and interstitial atmosphere for six months. Relative humidity of the hermetic bags remained below 25%, whereas temperature followed the same pattern as of the ambient. Germination percentage, thousand kernel weight and besatz content did not change significantly (p > 0.05), whereas mould count and moisture content reduced over the storage time compared wheat stored in metal bin.     

Quality of maize grains treated with allyl isothiocyanate stored in hermetic bags

This purpose of this study was to evaluate the quality of maize grain treated with allyl isothiocyanate (AITC) and stored under hermetic conditions. Maize grains with moisture contents of 14.8 or 17.9% wet basis (w.b.) initial moisture content were packaged in high density polyethylene bags and submitted to treatment with AITC at a concentration of 300 μL kg⁻¹. The bags were stored in controlled temperature chambers at 15, 25 or 35 °C. At 30-day intervals, for a period of 150 days, three bags were removed from the chambers and the grains were submitted to commercial classification, moisture content, bulk density, germination potential and electrical conductivity analyses. It was verified that AITC at 300 μL kg⁻¹ has no significant effect on the physiological quality of maize grains stored in hermetic bags. The microbiological analyses indicate the control of fungi and yeasts on the grains stored with 17.9% w.b. and treated with AITC. The maize grains stored with 14.8% w.b. moisture content in hermetic systems suffered no deterioration and retained their quality for a longer period than grains stored with 17.9% w.b. moisture content, independent of AITC treatment.