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磺胺甲恶唑酶联免疫分析法的建立 总被引:1,自引:0,他引:1
建立了磺胺甲恶唑(Sulfamethoxazole,SMX)酶联免疫分析方法,用以检测动物源性食品中SMX的残留。利用辣根过氧化物酶(HRP)标记SMX,兔抗SMX抗体制备固相抗体,通过标本中的SMX和一定量酶标SMX竞争结合固相抗SMX多抗,标本中SMX的量和显色后的OD450呈负相关的原理检测标本中的SMX含量。经方法学鉴定,本方法的灵敏度为0.067μg/L,批内变异系数<10%,批间变异系数<20%。牛奶、肉类、鸡蛋、蜂蜜样品的回收率分别为94.9%~116.0%、82.0%~107.6%、81.6%~94.9%、89.0%~93.0%,符合免疫分析方法的要求。 相似文献
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磺胺类药物是在农牧业和兽医临床中广泛应用的一种抗菌药,也用做饲料添加剂。但它在奶类和肉制品中的残留可能诱发细菌的耐药性,甚至可能致敏和致癌。目前,国内对动物源性食品中磺胺类药物残留监测主要为磺胺二甲嘧啶(SMZ)、磺胺二甲氧嘧啶(SDM)、磺胺间甲氧嘧啶(SMM)、磺胺喹恶啉(SQ)、磺胺甲恶唑(SMX)。在国内, 相似文献
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白蛋白酶联免疫分析试剂盒的研制 总被引:1,自引:1,他引:0
采用竞争性ELISA法检测人尿中白蛋白(Albumin,Alb)的含量。将Alb抗体包被96孔微孔板,Alb与辣根过氧化物酶结合形成酶标记物,建立了白蛋白酶联免疫分析(Alb-ELISA)方法。结果显示,本方法分析灵敏度为0.28 mg/L;批内、批间变异系数分别为2.63%~5.28%和2.40%~4.26%;回收率为95.0%~106.4%;高浓度Alb样品系列倍比稀释后,测定值与稀释度呈线性相关,相关系数为r=0.997 4。本试剂盒操作简便、快速,适用于临床检测和科研应用。 相似文献
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以β2-微球蛋白(β2-MG)抗体包被微孔板,四甲基联苯胺(TMB)为底物,用辣根过氧化物酶标记β2-MG,建立了β2-微球蛋白酶联免疫分析试剂盒,并对该方法进行了方法学分析。结果显示:本方法分析灵敏度为0.15 mg/L,批内、批间变异系数分别为4.1%~11.2%和14.1%~16.0%,回收率为93.3%~115.9%,高浓度β2-MG样品系列倍比稀释后,测定值与稀释度呈线性相关,相关系数为r=0.997 4。特异性结果显示:本试剂盒与铁蛋白基本无交叉反应,与白蛋白及甲胎蛋白在浓度分别低于10 mg/L及5 mg/L时有交叉反应,但反应很小。与放射免疫分析法(RIA)同时测定人血清样品,方法间有良好的相关性,相关方程为yELIA=1.006xRIA+0.406,r=0.900(n=59)。以上参数均符合临床免疫分析的要求。本试剂盒操作简便、快速,适用于临床检测和科研应用。 相似文献
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Shuheng HU 《等离子体科学和技术》2023,25(3):35510
Sulfamethoxazole (SMX) is an antibiotic and widely present in aquatic environments, so it presents a serious threat to human health and sustainable development. A dielectric barrier discharge (DBD) plasma jet was utilized to degrade aqueous SMX, and the effects of various operating parameters (working gas, discharge power, etc) on SMX degradation performance were studied. The experimental results showed that the DBD plasma jet can obtain a relatively high degradation efficiency for SMX when the discharge power is high with an oxygen atmosphere, the initial concentration of SMX is low, and the aqueous solution is under acidic conditions. The reactive species produced in the liquid phase were detected, and OH radicals and O3 were found to play a significant role in the degradation of SMX. Moreover, the process of SMX degradation could be better fitted by the quasi-first-order reaction kinetic equation. The analysis of the SMX degradation process indicated that SMX was gradually decomposed and 4-amino benzene sulfonic acid, benzene sulfonamide, 4-nitro SMX, and phenylsulfinyl acid were detected, and thus three possible degradation pathways were finally proposed. The mineralization degree of SMX reached 90.04% after plasma treatment for 20 min, and the toxicity of the solution fluctuated with the discharge time but eventually decreased. 相似文献
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《Progress in Nuclear Energy》2012,54(8):1148-1150
To determine allergenic foods, one of the most used tests is the immunoassays such as ELISA (enzyme-linked immunosorbent assay). The aim of this study was to determine the polyclonal antibody efficiency, produced in laboratory, to identify the presence the ovomucoid antigen in treated eggs by gamma irradiation for its inactivation. To evaluate the treatments, polyclonal antibody was produced. It was used, in nature, commercial laying eggs. Samples were submitted to the gamma radiation coming from a source of Co60, under a dose rate of 19.4 and 31.8 Gy/h, in the doses: 0 (control); 10 kGy; 20 kGy and 30 kGy, in all rates. By the ELISA’s test we can find the egg allergen ovomucoid and the radiation treatment do not showed considerable changes. So we can conclude that the antibody produced is capable of identify the ovomucoid allergenic protein and the gamma irradiation in such rates does not shows changes in that protein. 相似文献
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采用两株CA19-9单克隆抗体,一株用于125I标记、另一株包被于试管作为固相抗体,用血清稀释CA19-9抗原,制备标准品,建立了CA19-9双位点夹心免疫放射分析方法(IRMA)。分析采用两步法,本方法最小检测限为2.0 U/mL,批内、批间变异系数分别为 6.4%~9.5%和6.0%~12.6%,样品添加实验结果显示,回收率为88.1%~106.1%,血清样品倍比稀释后测定,测定值和稀释度的相关系数为0.990。CA19-9浓度至11800 U/mL时测定未见“弯钩”效应。69例健康人血清样品测定值为0.3~29.6 U/mL( ±s 为7.4±5.8 U/mL),和法国CIS公司的CA19-9免疫放射分析药盒同时测定84例人血清样品,二者测定值相关方程为y=1.32x-17.6,相关系数r=0.896。相似文献
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M.N.C. Harder V. ArthurS.G. Canniatti-Brazaca V.J.M. SavinoA.A.D. Coelho L.C.A.S. SilvaK.M.R. Duarte B.S. Rodriguez 《Progress in Nuclear Energy》2011,53(8):1148-1150
To determine allergenic foods, one of the most used tests is the immunoassays such as ELISA (enzyme-linked immunosorbent assay). The aim of this study was to determine the polyclonal antibody efficiency, produced in laboratory, to identify the presence the ovomucoid antigen in treated eggs by gamma irradiation for its inactivation. To evaluate the treatments, polyclonal antibody was produced. It was used, in nature, commercial laying eggs. Samples were submitted to the gamma radiation coming from a source of Co60, under a dose rate of 19.4 and 31.8 Gy/h, in the doses: 0 (control); 10 kGy; 20 kGy and 30 kGy, in all rates. By the ELISA’s test we can find the egg allergen ovomucoid and the radiation treatment do not showed considerable changes. So we can conclude that the antibody produced is capable of identify the ovomucoid allergenic protein and the gamma irradiation in such rates does not shows changes in that protein. 相似文献
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游离三碘甲腺原氨酸(FT3)生物素-亲合素酶联免疫分析 总被引:1,自引:1,他引:0
采用T3抗体包被酶标板微孔、生物素化T3和辣根过氧化物酶(HRP)标记链亲合素作为信号放大体系,一系列定量游离T3(FT3)为标准品,并以四甲基联苯胺-过氧化氢为显色底物,建立了FT3的生物素-亲合素(BA)酶联免疫分析(FT3-BA-EIA)法。其灵敏度为0.90pmol/L,标准曲线范围0.9~45.0pmol/L,批内变异系数3.1%~8.1%,批间变异系数4.9%~10.6%。测定了101例临床血样,其中正常人46例,实测范围2.5~11.1pmol/L,均值为5.1±2.0pmol/L;27例甲亢病人,实测范围9.7~37.3pmol/L,均值为20.5±7.1pmol/L;20例甲低病人,实测范围0.9~3.8pmol/L,均值为2.0±0.9pmol/L;8例孕妇,实测范围为3.4~8.5pmol/L,均值为6.0±2.5pmol/L。通过对临床血样的检测,本法与化学发光法(CIA)及放免法(RIA)的测定结果有良好的相关性。应用本法可有效地诊断甲状腺疾病,具有操作简便、灵敏、快速的特点,适于临床检测和科研应用。 相似文献
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建立了一种简便、快速的测定注射用亚锡亚甲基二膦酸盐冻干品中磷含量的分析方法,与中国药典2005版收录的方法比较,不需要通入硫化氢气体、离心、高温灼烧等步骤,简化了操作程序,缩短了分析时间,有利于生产中的质量控制。本方法的回收率为98.6%~101.3%,相对标准偏差 0.2%,与中国药典方法进行比较验证,结果较为满意。 相似文献
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研究探讨了制备和鉴定SARS冠状病毒表面抗原的快速免疫检测方法。采用基因工程表达SARS冠状病毒表面抗原免疫BALB/c小鼠,通过杂交瘤技术,获得了4株稳定分泌高特异性抗SARS冠状病毒表面抗原的杂交瘤细胞系,制取了含高效价单克隆抗体的腹水;采用SARS冠状病毒表面抗原免疫家兔、绵羊、山羊,制取了抗SARS冠状病毒表面抗原的多克隆抗体。研究结果表明:CS4C11单抗作为示踪抗体、绵羊抗SARS冠状病毒表面抗原抗体作为固相抗体为最佳配对;CS4C11单抗作为示踪抗体、CS4B12单抗作为固相抗体次之。获得的抗SARS冠状病毒表面抗原的抗体为SARS的早期诊断研究奠定了基础。 相似文献