Acrylamide–asparagine relationship in baked/toasted wheat and rye breads

Acrylamide in baked and toasted wheat and rye bread was studied in relation to levels of asparagine in flour, dough, bread and toasts. Asparagine was consumed during bread preparation resulting in reduced acrylamide content in the products. In wheat bread, 12% of the asparagine initially present in the flour (0.14 g kg^?1) remained after yeast fermentation and baking; for rye bread, 82% of asparagine remained after sourdough fermentation and baking. Asparagine present in untoasted wheat bread had totally reacted after hard toasting. Toasted wheat and rye bread slices contained 11–161 and 27–205 µg kg^?1 acrylamide, respectively, compared to untoasted wheat and rye bread with <5 and 7–23 µg kg^?1 acrylamide, respectively. The dietary intake of acrylamide from bread (untoasted) of 2 µg day^?1 is relatively low; however, acrylamide exposure from bread increases several fold for people eating toasted bread.     

Effect of flour extraction rate and baking process on vitamin B1 and B2 contents and antioxidant activity of ginger-based products

The effect of flour extraction rate and baking on thiamine (vitamin B₁) and riboflavin (vitamin B₂) content and antioxidant capacity of traditional ginger cake was studied and then compared to white wheat bread. Ginger cake was formulated either with whole-grain (100% extraction rate) or with brown (92% extraction rate) rye flour and baked at 180 °C for 18 min. The antioxidant capacity was evaluated in terms of radical scavenging activity against peroxyl (ROO·) and superoxide anion radicals (O ₂ ^·? ). Thiamine content in rye doughs (F-100% and F-92%) was found to be 38% lower when compared to wheat dough. In contrast, whole-grain and brown rye doughs exhibited an almost fourfold higher riboflavin content than wheat dough. Rye dough baking led to reductions in thiamine (from 53 to 65%) and riboflavin (from 69 to 71%) contents. Likewise, thiamine and riboflavin contents in wheat dough were also reduced (56 and 10%, respectively) after baking; however, ginger cake with whole-grain rye flour exhibited significantly higher thiamine and riboflavin contents. Rye doughs and ginger cakes showed higher scavenging activities against ROO· radicals when compared to that of wheat dough and bread. Thus, baking significantly enhanced ROO· scavenging properties of ginger cakes while only a slight increase was observed in wheat bread. In contrary, baking gave rise to a decrease in SOD-like activity both in ginger cake or wheat bread. Our findings suggest that formulation with whole-grain rye flour can potentially increase B₁ and B₂ vitamin contents as well as the ROO· scavenging capacity of traditional ginger cake.     

Occurrence of enniatins and beauvericin in 60 Chinese medicinal herbs

A total of 60 Chinese medicinal herbs were examined for contamination of the emerging Fusarium mycotoxins enniatins (ENNs) A, A1, B, B1 and beauvericin (BEA). The herbs under study are commonly used in China as both medicines and food. The dried samples of herbs were randomly collected from traditional Chinese medicine stores in Zhejiang province, China. Sample preparation was achieved by methanol extraction, followed by a simple membrane filtration step; no tedious clean-ups were involved. ENNs A, A1, B, B1 and BEA were analysed by the recently developed stable isotope dilution assays, using liquid chromatography-tandem mass spectrometry (LC-MS/MS). With limits of detection ranging between 0.8 and 1.2 µg kg^–1 for the analytes under study, 25% of all analysed samples were contaminated with at least one of the ENNs and BEA. BEA was the most frequently detected toxin with a 20% incidence in all samples. The percentages of ENN-positive samples were lower: each single ENN was detected in 6.7–11.7% of all samples. Considering the total amounts of the five mycotoxins in single samples, values between 2.5 and 751 µg kg^–1 were found. The mean total amount in positive samples was 126 µg kg^–1. Regarding ginger, the frequent occurrence of ENNs and BEA in dried ginger could be confirmed in samples from Germany. However, in fresh ginger root the toxins were not detectable. This is the first report on the presence of ENNs and BEA in Chinese medicinal herbs.     

Acrylamide-asparagine relationship in baked/toasted wheat and rye breads

Acrylamide in baked and toasted wheat and rye bread was studied in relation to levels of asparagine in flour, dough, bread and toasts. Asparagine was consumed during bread preparation resulting in reduced acrylamide content in the products. In wheat bread, 12% of the asparagine initially present in the flour (0.14 g kg(-1)) remained after yeast fermentation and baking; for rye bread, 82% of asparagine remained after sourdough fermentation and baking. Asparagine present in untoasted wheat bread had totally reacted after hard toasting. Toasted wheat and rye bread slices contained 11-161 and 27-205 microg kg(-1) acrylamide, respectively, compared to untoasted wheat and rye bread with <5 and 7-23 microg kg(-1) acrylamide, respectively. The dietary intake of acrylamide from bread (untoasted) of 2 microg day(-1) is relatively low; however, acrylamide exposure from bread increases several fold for people eating toasted bread.     

Effect of Flour Extraction Rate and Baking on Thiamine and Riboflavin Content and Antioxidant Capacity of Traditional Rye Bread

ABSTRACT:  The effect of rye flour extraction rates and baking on thiamine and riboflavin content, and antioxidant capacity of traditional rye bread were studied and compared with white wheat flour. The content of thiamine was higher (10.9%) in rye dough formulated with dark rye flour (F-100%; extraction rate of 100%) than in rye dough formulated with brown rye flour (F-92%; extraction rate of 92%) that was similar to dough made with wheat flour. The riboflavin content in rye dough made from flour F-100% was also higher (16%) than in dough formulated with flour F-92%, and both provided larger riboflavin content than wheat dough. Baking led to reductions in thiamine of 56% for wheat bread and of 20% for both rye breads; however, this process caused only a 10% decrease in riboflavin for wheat bread and a 30% decrease for rye breads. Trolox equivalent antioxidant capacity, peroxyl radical scavenging capacity, DPPH radical scavenging activity, and Folin–Ciocalteu reducing capacity were higher in rye than in wheat dough and bread. Baking process produced slight changes in antioxidant activity, except for Superoxide Dismutase-like activity where a sharp decrease was observed. Our findings showed that rye breads are an important source of B vitamins and rye breads formulated with dark and brown flours showed better antioxidant properties than wheat bread. Therefore, rye breads should be more widely recommended in human nutrition.     

Release of free ferulic acid and changes in antioxidant properties during the wheat and rye bread making process

Changes in the free ferulic acid (FFA) contents and antioxidant properties during bread making processes were determined. Experimental breads were produced from whole meal and white wheat and rye flours, and fermented using either baker’s yeast or sourdough starter. Sourdough fermented bread contained the highest content of FFA. Release of occurred mainly during dough fermentation. A further increase in the ferulic acid content in the bread crumb and a decrease in the crust was observed. Total antioxidant properties of sourdough bread, defined as the sum of lipophilic and hydrophilic compound activities, were significantly (p<0.05) higher than for yeast bread. Sourdough bread contained more methanol soluble phenolic compounds, proteins, tocochromanols, and oxidized products of fatty acids than yeast bread. The equilibrium between the anti- and pro-oxidative compound contents resulted in similar antioxidant properties for bread using both types of fermentation, and to results observed for the flour used for baking.     

Impact of sourdough fermented with Lactobacillus plantarum FST 1.7 on baking and sensory properties of gluten-free breads

Sourdoughs were produced from buckwheat, oat, quinoa, sorghum, teff and wheat flour using the heterofermentative lactic acid bacteria Lactobacillus plantarum FST 1.7 and added to a basic bread formulation of flour from the same grain type (20 % addition level). Dough rheology, textural (crumb hardness, specific volume) and structural bread characteristics (crumb porosity, cell volume, brightness) of sourdough-containing breads were compared to non-sourdough-containing breads (control). Changes in protein profiles as analysed with capillary electrophoresis were observed in all sourdoughs. Furthermore, sourdough addition led to decreased dough strength resulting in softer dough. No influences on specific volume and hardness on day of baking were found for gluten-free sourdough breads. The staling rate was reduced in buckwheat (from 8 ± 2 to 6 ± 2 N/day) and teff sourdough bread (13 ± 1 to 10 ± 4 N/day), however, not significantly in comparison with the control breads. On the contrary, in wheat sourdough bread, the staling rate was significantly reduced (2 ± 1 N/day) in comparison with control bread (5 ± 1 N/day). Sourdough addition increased the cell volume significantly in sorghum (+61 %), teff (+92 %) and wheat sourdough breads (+78 %). Therefore, crumb porosity was significantly increased in all gluten-free and wheat sourdough breads. Shelf life for sourdough breads was one (teff and oat), two (buckwheat, quinoa and sorghum) and 3 days (wheat) and was not prolonged by sourdough addition. The inferior aroma of breads prepared from the gluten-free flours was also not improved by sourdough addition.     

Effects of wheat sourdough process on the quality of mixed oat-wheat bread

The aim of this work was to study the effects of sourdough fermentation of wheat flour with Lactobacillus plantarum, on the quality attributes of mixed oat-wheat bread (51 g whole grain oat flour and 49 g/100 g white wheat flour). Emphasis was laid both on β-glucan stability as well as bread structure and sensory quality. The variables of the sourdough process were: dough yield (DY), fermentation time, fermentation temperature, and amount of sourdough added to the bread dough. The sourdough process was shown to be a feasible method for mixed oat-wheat bread, and, when optimized, provided bread quality equal to straight dough baking. A small amount (10g/100 g dough) of slack sourdough fermented at high temperature for a long time resulted in the most optimal sourdough bread with the highest specific volume (3.5 cm³/g), the lowest firmness after 3 days storage (0.31 kg), and low sensory sourness with high intensity of the crumb flavour. Wheat sourdough parameters did not affect the content of oat β-glucan in the bread. Additionally, both straight dough and sourdough bread contained 1.4-1.6 g β-glucan/100 g fresh bread. The average molecular weight of β-glucan was 5.5 × 10⁵ in both types of bread, while that of oat flour was 10 × 10⁵. This indicates that a slight degradation of β-glucan occurred during proofing and baking, and it was not affected by variation in the acidity of the bread between pH 4.9-5.8.     

The effects of low mixing temperature on dough rheology and bread properties

In this research, the effects of a low mixing temperature on dough rheology and the quality of bread were investigated. In the experiments, strong flour samples (Type 550), 1.5% salt, 3% of yeast and 1% additive mixture were used and dough samples were mixed at 17 °C (low temperature), 23 °C (control) and 30 °C (high temperature). Five different periods (0, 30, 60, 90 and 120 min) were applied at the bulk fermentation stage. At the final proofing stage, the dough was fermented until it reached a constant height. It was determined that almost every bread from dough samples mixed at 17 °C resulted in not only the highest bread volume and bread weight, but also the best texture, elasticity and crumb structure. The results of dough samples mixed at 23 °C were worse than those of dough samples mixed at 17 °C. The worst results were obtained from dough samples mixed at 30 °C (high temperature). As a result, it may be concluded that the quality of bread from dough samples mixed at low temperature (17 °C) is superior to those from dough samples mixed at the higher temperatures. Besides these findings, it may also be stated that prolonging the period of bulk fermentation in dough samples mixed at 17 °C positively develops baking performances.     

不同发酵基质的酸面团对酵母面团体系面包烘焙及老化特性的影响

应用分离自我国传统酸面团的区域特色乳酸菌--旧金山乳杆菌分别发酵小麦粉和小麦麸皮基质制成（小麦/麦麸）酸面团,研究了两种不同发酵基质的酸面团及其添加量对酵母面团体系面包烘焙及老化特性的影响。结果表明：与小麦粉制作的空白组面包相比,小麦酸面团可以明显改善面包的比容和感官品质;添加未发酵麦麸制作的非酸面团麦麸面包品质低于空白组,但引入麦麸酸面团（10%、20%、30%）后面包比容和感官评定得分均高于相对应的非酸面团麦麸面包。小麦酸面团和麦麸酸面团以及小麦麸皮均可以改善面包的老化特性,在相同贮藏期内,酸面团面包和麦麸面包的硬度增加量、水分迁移量和老化焓值都低于空白组,并且添加麦麸酸面团的面包其硬度和老化焓值都低于相对应的非酸面团麦麸面包。     

Overall and Local Bread Expansion,Mechanical Properties,and Molecular Structure During Bread Baking: Effect of Emulsifying Starches

In order to determine the relationship between molecular structure of wheat bread dough, its mechanical properties, total and local bread expansion during baking and final bread quality, different methods (rheological, nuclear magnetic resonance, magnetic resonance imaging and general bread characterisation) were employed. The study was extended on wheat dough with starch modified by octenyl succinic anhydride (OSA) in order to generalise the results. The interest of investigating multi-scale changes occurring in dough at different phases of baking process by considering overall results was demonstrated. It was found that OSA starch improved the baking performance during the first phase of baking. This feature was due to a higher absorption of water by OSA starch granules occurring at temperatures below that of starch gelatinization, as confirmed by NMR, and consecutive higher resistance to deformation for OSA dough in this temperature range (20–60 °C). This was explained by a delayed collapse of cell walls in the bottom of the OSA dough. In the second phase of baking (60–80 °C), the mechanism of shrinkage reduced the volume gained by OSA dough during the first phase of baking due to higher rigidity of OSA dough and its higher resistance to deformation. MRI monitoring of the inflation during baking made it possible to distinguish the qualities and defaults coming from the addition of OSA starch as well as to suggest the possible mechanisms at the origin of such dough behaviour.     

Changes in ochratoxin A and type B trichothecenes contained in wheat flour during dough fermentation and bread-baking

Ochratoxin A (OTA) and type B trichothecenes are mycotoxins that occur frequently in cereals and thus can be found in cereal by-products such as bread. The aim of this work was to study the variation of the levels of OTA, deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON) and nivalenol (NIV) during the bread-making process. This was done by using wheat flour spiked with different levels of toxins. Mycotoxin levels were controlled after fermentation of the dough with yeasts (Saccharomyces cerevisiae) and after further baking at different temperature–time combinations. Analysis of variance (ANOVA) of the results showed a significant reduction in OTA level (p < 0.05) during fermentation of the dough. The reduction ranged between 29.8% and 33.5%, depending on the initial concentration of toxin in the flour. During this period, the level of the other mycotoxins studied was not modified. By contrast, in the baking phase there were significant changes in the levels of the four mycotoxins, although the reduction was similar under all the baking conditions. Considering all the temperature–time conditions tested, it can be concluded that during the baking period the average reduction of OTA, NIV, 3-ADON, and DON was 32.9%, 76.9%, 65.6%, and 47.9%, respectively.     

Identification of lactic acid bacteria isolated from oat sourdoughs and investigation into their potential for the improvement of oat bread quality

The use of sourdough in wheat and rye breads has been extensively studied; however, little is known about its potential effect when baking oat bread. Consequently, the impact of sourdough on oat bread quality was investigated. Two different sourdoughs were prepared from wholegrain oat flour without the addition of starter cultures, by continuous propagation at 28 (SD 28) or 37 °C (SD 37) until the composition of the lactic acid bacteria remained stable. The dominant LAB were identified by sequence analysis of the 16S rDNA isolated from pure cultures. LAB from SD 28 belonged to the species Leuconostoc argentinum, Pedicoccus pentosaceus and Weissella cibaria, while Lactobacillus coryniformis dominated SD 37. The isolated LAB were further used as starter cultures for the production of oat sourdoughs. Fundamental rheology revealed softening of the sourdoughs compared to non-acidified and chemically acidified controls, which could not be attributed to proteolytic activity. Incorporation of oat sourdough into an oat bread recipe resulted in significantly increased loaf-specific volume as well as improved texture, independent of addition level or sourdough type. Overall, the results of this study show that sourdoughs containing lactic acid bacteria isolated from oats have the potential to enhance oat bread quality.     

The bacteriocin producer Lactobacillus amylovorus DCE 471 is a competitive starter culture for type II sourdough fermentations

Type II sourdoughs were prepared using Lactobacillus amylovorus DCE 471, a producer of the bacteriocin amylovorin L. The strain was used as a starter culture for rye and wheat sourdoughs on laboratory scale (10 L), and in rye sourdough on pilot scale (100 L). The sourdoughs were acidified to a pH of around 3.5 within 15 h (laboratory dough) to 25 h (pilot‐scale dough). Final amylovorin L titres of 0.3–0.4 (laboratory scale) and 0.2 (pilot scale) MAU kg^?1 of sourdough were detected. After baking of wheat dough that was supplemented with the pilot‐scale sourdough, no amylovorin L activity was recovered from the breadcrumbs. On laboratory scale, aeration or the addition of complex carbohydrates hardly affected growth or amylovorin L production. Rye and wheat sourdough fermentation were rather similar despite differences in sugar concentrations. The persistence of L. amylovorus DCE 471 during rye sourdough fermentation, both on laboratory and pilot scale, was confirmed by repetitive sequence‐based polymerase chain reaction (rep‐PCR) and by testing isolates towards an amylovorin L‐sensitive organism. Further, rep‐PCR indicated that the background microbiota of the flour—probably responsible for the production of low amounts of acetic acid—grew poorly and were overgrown by L. amylovorus DCE 471 during the pilot‐scale fermentation. Copyright © 2007 Society of Chemical Industry     

Effect of Wheat Bran,Coarse Wheat Flour,and Rice Flour on the Instrumental Texture of Cookies

Abstract

The effect of replacing wheat flour with wheat bran (0–10%), coarse wheat flour (0–20%) or with rice flour (0–20%) on the quality of cookies was studied. The cookie dough was subjected to Instrumental Texture Profile Analysis in order to determine dough cohesiveness and adhesiveness. After baking spread factor, puncture force and fracture strength of the cookies was also determined. The control dough had cohesiveness and adhesiveness values of 0.279 and 13.6 N s whereas control cookies had puncture force and fracture strength of 90.84 N and 100.16 N. Wheat bran increased dough cohesiveness and adhesiveness whereas coarse wheat flour had the opposite effect. Increasing levels of rice flour decreased cohesiveness but increased adhesiveness. Wheat bran and rice flour lowered the spread factor where as coarse wheat flour increased spread factor. Coarse wheat flour and rice flour lowered the fracture strength where as wheat bran increased fracture strength. Sensory evaluation revealed that increasing levels of wheat bran lowered the overall acceptability whereas rice flour and coarse wheat flour improved sensory scores.     

Exploitation of buckwheat sourdough for the production of wheat bread

This study investigates the exploitation of buckwheat sourdough for the production of wheat bread. The fermentation induced extensive hydrolysis of buckwheat main storage proteins, but did not influence the total protein, starch and polyphenols content of buckwheat. Buckwheat sourdough was incorporated at 10 and 20?% (w/w) in wheat dough, and control doughs were produced with the addition of a chemically acidified (CA) buckwheat batter. The addition of buckwheat sourdough greatly affected the rheological properties of the dough, by inducing a strengthening of the gluten network and decrease in elasticity. The acidification of wheat dough also stimulated the baker’s yeast activity during proofing, resulting in higher release of CO₂ in shorter times (volume of CO₂ released (ml), control dough, 1,671.5; dough with 10?% sourdough, 2,600; dough with 10?% chemically acidified dough, 2,715.5). The properties of wheat bread were enhanced by the addition of 10?% buckwheat sourdough, which led to higher specific volume (control, 3.41?ml/g; bread with 10?% sourdough, 4.03?ml/g) and softer crumb (crumb hardness, control, 5.28?N; bread with 10?% sourdough, 3.93?N). On the other hand, the higher acidification level did not influence the bread volume, but slightly hardened the crumb (crumb hardness, bread with 20?% sourdough, 7.41?N; bread with 20?% chemically acidified dough, 6.48?N). The fermentation positively influenced the nutritional properties of buckwheat flour and wheat bread, in terms of polyphenols (control bread, 8.84?mg GAE/100?g; bread with 10 and 20?% sourdough, 17.83 and 18.20?mg GAE/100?g, respectively) and phytic acid contents. Incorporation of buckwheat sourdough also led to an extension in the shelf life of wheat bread, which became more evident for the higher addition level. Overall, the results of this study suggest that buckwheat sourdough represents a suitable tool for enhancing the overall quality and nutritional properties of wheat bread.     

Sourdough in gluten-free bread-making: An ancient technology to solve a novel issue?

The increasing demand for high quality gluten-free (GF) bread, clean labels and natural products is raising the need for new approaches in GF bread-making. Sourdough is the foremost fermentation used for baking purposes and it has been proven to be ideal for improving the texture, palatability, aroma, shelf life and nutritional value of wheat and rye breads. These characteristic features derive from the complex metabolic activities of the sourdough-resident lactic acid bacteria and yeasts, e.g. acidification, production of exopolysaccharides, proteolytic- amylolytic- and phytase activity, and production of antimicrobial substances. These effects have been extensively studied and well described for traditional baking, whereas little is known about the role of sourdough in GF baking. Yet, the microbiological and qualitative characterisation of local GF fermented products indicate an overlap with the microbiota of wheat/rye fermentation and suggest that the positive metabolic activities of the sourdough microbiota are still retained during fermentation of GF crops. Thus, the use of sourdough in GF baking may be the new frontier for improving the quality, safety and acceptability of GF bread.     

Influence of enzyme treatment on the rheology of rye doughs

The rheological behaviour of rye dough is mainly influenced by enzymatic reactions. An investigation with a rotation rheometer called a rheological baking test was used to study the influence of pentosanase, proteinase, fungal α-amylase, and bacterial α-amylase, respectively. The application of pentosanase increased the plasticity of the dough and decreased the viscosity. Proteinase had only a slight influence on the rheological behaviour of rye dough. It slightly decreased the dough viscosity, but had no influence on the visco-elasticity. The fungal and the bacterial α-amylase showed the same effects. The dough elasticity decreased with the increase in temperature. These effects are obvious even at temperatures below gelatinization, and the bacterial amylase shows a greater influence, because it can work at higher temperatures. It could be concluded that the dough structure is mainly built up by pentosans and proteins at temperatures of 30°C, whereas the starch plays a major role in the temperature range above 45°C. A bread baking test showed that pentosanase had an effect on the shape of the loaf whereas the amylases influenced the volume and the crumb elasticity. Proteinase showed no influence on baking results. This correponds well with the results of the rheological investigations. The rheological baking test is a good tool for predicting the baking performance of rye flour.     

The microflora of sourdough. XVIII. The protein degrading capabilities of lactic acid bacteria in sourdough

Acidification of the dough by the use of sourdough or acidifiers is necessary not only for good baking quality of rye flour but it is also very important for development of the typical sensory characteristics of rye bread. We confirmed that the lactic acid bacteria of sour dough are proteolytic. Proteolytic effects are observed in the increase of the amino acid content during fermentation. A marked increase was found in the content of leucine, alanine, valine, isoleucine, glutamic acid, glutamine, arginine, lysine, methionine, phenylalanine, tyrosine and serine. Lactobacillus plantarum showed a higher proteolytic activity than L. brevis ssp. lindneri or L. fructivorans.     

Sourdough-type bread from waste bread crumb

Optimal conditions for sourdough production from waste bread containing sugar were studied. Using a dough prepared with 50% white bread crumb in water instead of 35% crumb, high levels of acidity were formed at 35°C with a commercial lyophilized starter containingLactobacillus plantarum. The effect of temperature (25 or 35°C) on titratable acidity development was equivalent to crumb concentration (35 or 50%). Cell growth stopped after 12–24 h but acid production continued for more than 48 h. Best results were obtained with whole wheat bread crumb followed by sweet-type crumb (about 8% sugar, dry basis) and white bread crumb. Two commercial sources ofLactobacillus plantarum, one meat starter and one bread starter, were screened based on acid production in fermented bread crumb.