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1.
The effect of high hydrostatic pressure (HHP, 100–400 MPa) for 20 min at 25 °C to sweet potato flour (SPF) on dough properties and characteristics of sweet potato‐wheat bread was investigated. The particle size of SPF after HHP was decreased significantly. The obvious rupture was observed in granules of SPF after HHP at 300 and 400 MPa by scanning electron microscopy (SEM). After HHP, significant differences on endothermic peak temperatures (TP) of SPF were observed by differential scanning calorimetric (DSC), of which the enthalpy change (ΔH) had a slight increase, expect that at 200 MPa. Gas retention of dough with SPF after HHP increased markedly from 1199 (0.1 MPa) to 1246 ml (100 MPa). Specific loaf volume of bread with SPF at 400 MPa was increased significantly, while the hardness and chewiness were reduced. Thus, SPF treated with HHP at 400 MPa could be potentially used in wheat bread production.  相似文献   

2.
BACKGROUND: Changes in instrumental color parameters, chlorophyll a and b, activity of chlorophyllase, Mg‐dechelatase, peroxidase and polyphenol oxidase, total phenolic compounds and pH of spinach purée were assessed after high hydrostatic pressure (HHP) (200, 400 and 600 MPa for 5, 15 and 25 min) treatments at room temperature. RESULTS: HHP treatments induced a better retention of visual green color (?a* and L* values) and chlorophyll contents of spinach purée. As for chlorophyll degradation‐related enzymes, the results indicated that chlorophyllase activity decreased at all pressures; however, Mg‐dechelatase activity was dramatically activated after HHP treatment at 400 and 600 MPa. Peroxidase exhibited higher resistance to HHP; however, polyphenol oxidase, which is responsible for enzymatic browning, was suppressed progressively with increase in pressure level from 200 to 600 MPa. In addition, the pH value of HHP‐treated spinach purée was increased to be close to neutral pH, which could effectively inhibit chlorophyll degradation. No significant differences (P > 0.05) were found after extending the treatment times at the same level of pressure. CONCLUSION: HHP treatments effectively prevent chlorophyll degradation and enzymatic browning in spinach purée and retain a better original fresh green color of spinach compared with conventional thermal treatment. Copyright © 2012 Society of Chemical Industry  相似文献   

3.
Salmorejo is a traditional tomato‐based creamy product. Because salmorejo is not heat‐processed, there is a risk of contamination with foodborne pathogens from raw materials. Even though bacterial growth in salmorejo is strongly inhibited because of its acidic pH (close to 3.9), the growth and survival of 3 foodborne pathogens in this food has not been studied before. In this study, 3 cocktails consisting of Escherichia coli O157, Salmonella enterica serovar Enteritidis, and Listeria monocytogenes strains were inoculated in freshly prepared salmorejo. The food was treated by high hydrostatic pressure (HHP) at 400, 500, or 600 MPa for 8 min, or left untreated, and stored at 4 °C for 30 d. Viable cell counts were determined on selective media and also by the triple‐layer agar method in order to detect sublethally injured cells. In control samples, L. monocytogenes viable cells decreased by 2.4 log cycles at day 7 and were undetectable by day 15. S. enterica cells decreased by 0.5 or 2.4 log cycles at days 7 and 15 respectively, but still were detectable at day 30. E. coli O157 cells survived much better in salmorejo, decreasing only by 1.5 log cycles at day 30. Treatments at pressures of 400 MPa or higher reduced viable counts of L. monocytogenes and S. enterica to undetectable levels. HHP treatments significantly (P < 0.05) reduced E. coli counts by approximately 5.2 to 5.4 log cycles, but also yielded surviving cells that apparently were sublethally injured. Only samples treated at 600 MPA for 8 min were devoid of detectable E. coli cells during storage.  相似文献   

4.
The purpose of the study was to determine the effect of high‐hydrostatic pressure (HHP) on inactivation of human norovirus (HuNoV) in oysters and to evaluate organoleptic characteristics of oysters treated at pressure levels required for HuNoV inactivation. Genogroup I.1 (GI.1) or Genogroup II.4 (GII.4) HuNoV was inoculated into oysters and treated at 300 to 600 MPa at 25 and 0 °C for 2 min. After HHP, viral particles were extracted by porcine gastric mucin‐conjugated magnetic beads (PGM‐MBs) and viral RNA was quantified by real‐time RT‐PCR. Lower initial temperature (0 °C) significantly enhanced HHP inactivation of HuNoV compared to ambient temperature (25 °C; P < 0.05). HHP at 350 and 500 MPa at 0 °C could achieve more than 4 log10 reduction of GII.4 and GI.1 HuNoV in oysters, respectively. HHP treatments did not significantly change color or texture of oyster tissue. A 1‐ to 5‐scale hedonic sensory evaluation on appearance, aroma, color, and overall acceptability showed that pressure‐treated oysters received significantly higher quality scores than the untreated control (P < 0.05). Elevated pressure levels at 450 and 500 MPa did not significantly affect scores compared to 300 MPa at 0 °C, indicating increasing pressure level did not affect sensory acceptability of oysters. Oysters treated at 0 °C had slightly lower acceptability than the group treated at room temperature on day 1 (P < 0.05), but after 1 wk storage, no significant difference in sensory attributes and consumer desirability was observed (P > 0.05).  相似文献   

5.
The effect of high hydrostatic pressure (HHP) treatment on the structure, physicochemical and functional properties of cumin protein isolate (CPI) was investigated. More aggregates, pores, irregular conformations and bigger particle size were observed for HHP-treated CPI. HHP resulted in an increase in α-helix, a decrease in β-strand and fluorescence intensity of CPI. Surface hydrophobicity (Ho) of CPI significantly increased after HHP treatment, from 343.35 for native CPI to 906.22 at 600 MPa (P < 0.05). HHP treatment at 200 MPa reduced zeta-potential and solubility of CPI, while had little effect at 400 and 600 MPa. Emulsifying activity and stability of CPI decreased after HHP treatment, of which droplet size of emulsions significantly increased (P < 0.05). HHP-treated CPI could form heat-induced gelation at lower temperature (68.5 °C) and improved storage modulus (G′) comparing to native one (80.6 °C), suggesting that CPI might be potential protein resources as gelation substitute in food system.  相似文献   

6.
High hydrostatic pressure (HHP) is used for microbial inactivation in foods. Addition of carbon dioxide (CO2) to HHP can improve microbial and enzyme inactivation. This study investigated microbial effects of combined HHP and CO2 on Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, and evaluated sensory attributes of treated feijoa fruit puree (pH 3.2). Microorganisms in their growth media and feijoa puree were treated with HHP alone (HHP), or saturated with CO2 at 1 atm (HHPcarb), or 0.4%w/w of CO2 was injected into the package (HHPcarb+CO2). Microbial samples were processed at 200 to 400 MPa, 25 °C, 2 to 6 min. Feijoa samples were processed at 600 MPa, 20 °C, 5 min, then served with and without added sucrose (10%w/w). Treated samples were analyzed for microbial viability and sensory evaluation. Addition of CO2 enhanced microbial inactivation of HHP from 1.7‐log to 4.3‐log reduction in E. coli at 400 MPa, 4 min, and reduction of >6.5 logs in B. subtilis (vegetative cells) starting at 200 MPa, 2 min. For yeast, HHPcarb+CO2 increased the inactivation of HHP from 4.7‐log to 6.2‐log reduction at 250 MPa, 4 min. The synergistic effect of CO2 with HHP increased with increasing time and pressure. HHPcarb+CO2 treatment did not alter the appearance and color, while affecting the texture and flavor of unsweetened feijoa samples. There were no differences in sensory attributes and preferences between HHPcarb+CO2 and fresh sweetened products. Addition of CO2 in HHP treatment can reduce process pressure and time, and better preserve product quality.  相似文献   

7.
The influence of high hydrostatic pressure (HHP) treatment on the physicochemical and emulsifying properties of sweet potato protein (SPP) at various concentrations, e.g. 2%, 4% and 6% (w/v, SPP‐2, SPP‐4 and SPP‐6), was investigated. Significant differences in hydrophobicity, enthalpy of denaturation and solubility were observed (< 0.05). Emulsifying activity indexes (EAI) of SPP‐2 and SPP‐6 increased at 400 MPa, whereas EAI of all SPP significantly decreased at 600 MPa (< 0.05). Emulsion stability (ESI) was significantly decreased for SPP‐2 and SPP‐6, while increase in ESI was observed for SPP‐4 above 200 MPa (< 0.05). SPP‐2 emulsions showed sharp decrease in apparent viscosity with pressure increase, while pseudo plastic flow behaviour was not changed for all of emulsions. Sporamins A and B were well‐adsorbed in pressurised emulsion without displacement. These results suggest that HHP treatment could be used to modify the physicochemical and emulsifying properties of SPP.  相似文献   

8.
The inactivation of bacteria naturally present in strawberry pulp was investigated after high hydrostatic pressure (HHP) treatment at pressure levels up to 600 MPa at 25 °C for 5 ~ 25 min. Five strains of pressure‐resistant bacteria designated as A, B, C, D and E were isolated and identified. The five strains were gram‐positive, spore‐forming, rods or rod in chains. Growth of the strains was observed at 30 ~ 45 °C, and strain B also grew well at 55 °C. They could produce acid from glucose and were catalase‐positive. Analysis of 16S rRNA gene sequences showed that the five strains belonged to the genus Bacillus. Strain A and D exhibited the greatest 16S rRNA gene sequence similarity of 99% with B. licheniformis and B. firmus, respectively. By combination of phenotypic characteristics and 16S rRNA gene sequences, strain C was B. mycoides and E was B. pumilus. On the basis of physiological and biochemical characteristics, gyrB gene sequences analysis and whole‐cell fatty acids analysis, strain B was B. amyloliquefaciens. Further studies showed that strain B (B. amyloliquefaciens) exhibited the highest pressure resistance, and it was reduced by 4.62‐log after treatment at 600 MPa for 25 min at 25 °C as the most effective observed inactivation.  相似文献   

9.
This aim of the study was to evaluate the technological properties of rice starch modified by high hydrostatic pressure (HHP). Black rice starch (BRS) was dispersed in 20% water and then HHP was applied at pressures of 200, 400 and 600 MPa for 30 min, where morphological, structural, functional and thermal parameters were evaluated. High pressure (BRS600) provided greater morphological damage, such as surface cavities and loss of crystallinity. The treatment HHP > 400 MPa the type of diffraction pattern was changed from type A to type V. The FT-IR spectra showed differences in intensity, especially for control, which revealed better defined peaks of greater intensity. The modified starch showed a greater affinity for water and oil absorption than the native starch as well as for milk absorption, exhibiting a higher binding capacity for the whole milk. HHP treatment is a fast and efficient non-thermal method to improve the technological properties of BRS.  相似文献   

10.
The combined inactivation effects of high hydrostatic pressure (HHP) and antimicrobial compounds (potassium sorbate and ε‐polylysine [ε‐PL]) on 4 different bacterial strains present in skim milk and the effect of these treatments on milk quality were investigated in this study. HHP treatment at 500 MPa for 5 min reduced the populations of Escherichia coli, Salmonella enterica Typhimurium, Listeria monocytogenes, and Staphylococcus aureus from 6.5 log colony‐forming units (CFUs) or higher to less than 1 log CFU/mL. Compared to HHP alone, HHP with potassium or ε‐PL resulted in significantly higher reductions in the bacterial counts. After 5 min of treatment with HHP (500 MPa) and ε‐PL (2 mg/mL), no growth of E. coli, S. enterica Typhimurium, or L. monocytogenes in skim milk was observed during 15 d of refrigerated storage (4 ± 1 °C). Scanning electron microscopy analysis revealed that the synergistic treatments caused more serious damage to the bacterial cell walls. Quality assessments of the treated samples indicated that the combined treatments did not influence the color, the turbidity, the concentrations of –SH group of the proteins, or the in vitro digestion patterns of the milk. This study demonstrates that HHP with potassium or ε‐PL may be useful in the processing of milk or milk‐containing foods.  相似文献   

11.
The efficiency of solid–liquid extraction (SLE) and pressurised liquid extraction (PLE) for the recovery of antioxidant and polyphenols from the Irish macroalgae, Fucus serratus, Laminaria digitata, Gracilaria gracilis and Codium fragile, was assessed using the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays and the Folin–Ciocalteu total phenol content (TPC) assay. Fucus serratus had TPC and antioxidant activities thirty times higher than the other species. Solid–liquid extraction cold water (CWSLE) had the highest TPC (81.17 μg GAE mg?1 sample) derived from F. serratus, compared with the TPC of 61.12 μg GAE mg?1 sample for the corresponding PLE extract. For both SLE and PLE extracts, low TPC levels were observed in L. digitata, G. gracilis and C. fragile. The majority of SLE extracts possessed higher FRAP and DPPH activities compared with their PLE counterparts. This study indicates that the high temperatures and pressures in PLE did not enhance the antioxidant activities relative to conventional SLE extraction.  相似文献   

12.
High hydrostatic pressure (HHP) treatments (singly or in combination with natural antimicrobials) were tested for stabilization of Manzanilla Aloreña seasoned olives stored at 25 °C. HHP (5 min) was highly effective on yeast populations at 300 MPa or higher. No viable yeasts were detected in samples treated at 400 MPa for up to three months. Low levels of endospore-forming bacteria were always detected after HHP treatments. Addition of nisin to the brines reduced bacterial counts by 1.4 log cycles but it had no effect on yeasts when tested singly or in combination with HHP treatment (400 MPa, 5 min). Thyme oil had almost no effect on yeast concentrations, but rosemary oil reduced yeast viable counts progressively during storage. Essential oils in combination with HHP (400 MPa, 5 min) significantly (p < 0.05) reduced the concentrations of aerobic mesophilic bacteria. Low-salt brined olives purged with N2 or supplemented with ascorbic acid and then pressurized for 5 min at 450 or 550 MPa were preserved for up to 5 months without spoilage, suggesting that the NaCl content in brines of packed Manzanilla Aloreña table olives could be reduced considerably by application of HHP as a stabilization treatment.  相似文献   

13.
The survival curves of Escherichia coli and Listeria innocua inactivated by high hydrostatic pressure (HHP) were obtained at room temperature (∼22 °C) and at five pressure levels (400, 450, 500, 550 and 600 MPa) in whole milk. These curves were described by the Weibull model and parameters of this model were reduced from two to one with slight loss of goodness-of-fit. The logarithm of the time constant parameter (δ) of the reduced Weibull model was described with respect to high pressure (P). This approach can be used to define a z p value analogous to the modeling of the classical D value (increase in pressure that results in one log unit decrease of δ values). The development of accurate survival models under high pressure, as presented here, can be very beneficial to food industry for designing, evaluating and optimizing HHP processes as a new preservation technology.  相似文献   

14.
A recently proposed Gompertz model (GMPZ) approach describing microbial inactivation kinetics by high‐pressure processing (HPP) incorporated the initial microbial load (N0) and lower microbial quantification limit (Nlim), and simplified the dynamic effects of come‐up time (CUT). The inactivation of Listeria innocua in milk by HPP treatments at 300, 400, 500, and 600 MPa and pressure holding times (thold) ≤10 min was determined experimentally to validate this model approach. Models based on exponential, logistic‐exponential, and inverse functions were evaluated to describe the effect of pressure on the lag time (λ) and maximum inactivation rate (μmax), whereas the asymptote difference (A) was fixed as = log10(N0/Nlim). Model performance was statistically evaluated and further validated with additional data obtained at 450 and 550 MPa. All GMPZ models adequately fitted L. innocua data according to the coefficient of determination (R≥ 0.95) but those including a logistic‐exponential function for μmax(P) were superior (R≥ 0.97). These GMPZ versions predicted that approximately 597 MPa is the theoretical pressure level (Pλ) at which microbial inactivation begins during CUT, mathematically defined as λ (Pλ) = tCUT, and matching the value observed on the microbial survival curve at 600 MPa. As pressure increased, predictions tended to slightly underestimate the HPP lethality in the tail section of the survival curve. This may be overseen in practice since the observed microbial counts were below the predicted log10 N values. Overall, the modeling approach is promising, justifying further validation work for other microorganisms and food systems.  相似文献   

15.
BACKGROUND: Walnut (Juglans regia L.) is a good source of protein that has potential application in new product formation and fortification. The main objectives of this study were to investigate the effects of high hydrostatic pressure (HHP) treatment (300–600 MPa 20 min) on physicochemical and functional properties of walnut protein isolate (WPI) using various analytical techniques at room temperature. RESULTS: The results showed significant modification of solubility, free sulfhydryl content and surface hydrophobicity with increased levels of HHP treatment, indicating partial denaturation and aggregation of proteins. Differential scanning calorimetry and fluorescence spectrum analyses demonstrated that HHP treatment resulted in gradual unfolding of protein structure. Emulsifying activity index was significantly (P < 0.05) increased after HHP treatment at 400 MPa, but significantly decreased (P < 0.05) relative to the untreated WPI with further increase in pressure. HHP treatment at 300–600 MPa significantly decreased emulsion stability index. Additionally, HHP‐treated walnut proteins showed better foaming properties and in vitro digestibility. CONCLUSION: These results suggest that HHP treatment could be applied to modify the properties of walnut proteins by appropriate of pressure levels, which will help in using walnut protein as a potential food ingredient. © 2012 Society of Chemical Industry  相似文献   

16.
Fresh eggs from hens fed diets supplemented with 4% linseed oil (LO) or sunflower oil (SO) were either directly submitted to pasteurisation, hard‐boiling or scrambling processing, or first submitted to refrigerated storage at 4 °C for 60 day and then to processing. Fresh LO eggs showed higher (P ≤ 0.05) proportions of monounsaturated fatty acids (MUFAs) and n‐3 polyunsaturated fatty acids (PUFAs), but lower (P ≤ 0.05) proportions of saturated fatty acids (SFAs), PUFAs and n‐6 PUFAs than the SO eggs. Storage decreased (P ≤ 0.05) the proportion of PUFAs and increased (P ≤ 0.05) that of MUFAs in egg yolks from both treatments. The pasteurisation process had no effect on the fatty acid composition of fresh eggs from both treatments, but increased (P ≤ 0.05) n‐6 PUFAs and decreased (P ≤ 0.05) n‐3 PUFAs in stored LO eggs. Hard‐boiling and scrambling modified the fatty acid composition of fresh and stored eggs from both treatments by decreasing (P ≤ 0.05) the proportion of PUFAs, particularly of the very long‐chain n‐3 eicosapentaenoic, docosapentaenoic and docosahexaenoic PUFAs. LO eggs showed a higher susceptibility to fatty acid modification upon processing as compared to the SO eggs.  相似文献   

17.
Escherichia coli cells in peptone water were pressurized at 300 MPa at ambient temperature with no holding time (pulse series) and with a total holding duration of 300 s for single- (300 s × 1 pulse) and multi-pulsed (150 s × 2 pulses, 100 s × 3 pulses, 75 s × 4 pulses, 60 s × 5 pulses, 50 s × 6 pulses and 30 s × 10 pulses) high hydrostatic pressure (HHP) treatments. Multi-pulsed HHP treatment with no holding time indicated that as the pulse number increased the number of inactivated and injured cells also increased. Holding time had significant effect on the inactivation of E. coli. There was low inactivation difference between single- and multi-pulsed HHP treatments with holding time. Escherichia coli cells showed at least 1.6 log10 more reduction on selective medium than the non-selective medium indicating that more than 95 % of the survivors severely injured for both single- and multi-pulsed treatments with holding time. Although the inactivation difference was low between single- and multi-pulsed HHP treatments, storage at 4 °C revealed that there was less recovery from injury for multi-pulsed HHP treatment.  相似文献   

18.
Wild Lonicera caerulea berries were subjected to five different high hydrostatic pressure (HHP) treatments (which resemble the conditions of active component extraction and commercial sterilization). The content of anthocyanins and total phenolics increased by 6.84% and 14.35% (p < 0.05), respectively after treatment at 200 MPa for 5 and 10 min. As HHP increased, a higher loss of active component was observed. The total phenolic contents did not differ significantly between the control and the 400 MPa/20 min treated group (p > 0.05); HHP processing demonstrated better sterilization effect but severely destroyed enzymes. Polyphenol oxidase (PPO) and peroxidase (POD) activity were activated at lower HHP, such as 200 MPa, and decreased at 400–600 MPa. Superoxide dismutase (SOD) maintained good stability under HHP processing. The antioxidant capacities of anthocyanins extracts of wild L. caerulea berry were evaluated by 3 different methods (DPPH assay, oxygen radical absorbance capacity assay, and cellular antioxidant activity assay).Industrial RelevanceFactors such as color, luster, and nutrition often affect consumer choice in food. However, the color and nutrition of foods tend to be destroyed during processing and storage. The demand for healthier and more nutritious food while retaining the color and flavor after processing highlights the need to develop novel and gentler technologies for fruit processing. Recently, high hydrostatic pressure (HHP) technologies have been used in different branches of the food industry. In the present study, the content of active component in blue honeysuckle fruit pulps such as anthocyanins and polyphenols showed tendency to increase and then decrease with increasing pressure at room temperature. Five different HHP treatment groups (resembling the conditions of active component extraction conditions and commercial sterilization) were compared to the control (fresh fruit) and heat-treated group to determine the effects of HHP processing on L. caerulea berry pulps. The aim of this study was to investigate the changes in active component particularly the content and composition of anthocyanins under different high-pressure treatment at room temperature; the color and physicochemical indexes were also analyzed at the same conditions. Low HHP for a long period of time (400 MPa/20 min) demonstrated better results than that with high HHP for a short time (600 MPa/10 min), as indicated by the higher contents of anthocyanins and phenols and stronger antioxidant capacities. Therefore, Low HHP conditions can be used as an auxiliary means of active component extraction. The conditions of HHP processing at low HHP for a long period of time (400 MPa/20 min) can be altered to retain active components during food processing.  相似文献   

19.
High hydrostatic pressure (HHP) treatments can improve the potential of orange, mango, and prickly pear peels as food formulation fiber sources. Akaike Information Criteria differences identified Peleg and GAB as the best model alternatives to describe experimental moisture isotherms. HHP (600 MPa/10 min/22 and 55 °C) effects on moisture isotherms expressed as relative water sorption content change with respect to controls (RWSCaw) showed that in the 0.1–0.93 aw range, HHP improved the adsorption water retention of orange peels. The same was true for the desorption water retention for all HHP-treated fruit peels except for prickly pear HHP-treated at 22 °C and > 0.35 aw. The area under the hysteresis curve (AH) in the 0.15–0.51 aw range showed that HHP increased hysteresis for all fruit peels tested. All this illustrates the HHP potential to modify the hygroscopic properties of fruit peels at lower temperature and in less processing time than conventional processes.Industrial relevanceOrange, mango, and prickly pear peels are potential food fiber formulation sources with differentiated hygroscopic and functional properties. In this study, 600 MPa treatments at 22 and 55 °C for 10 min modified the adsorption and desorption moisture retention capacity of all fruit peels tested in this study. HHP technology can improve the potential of fruit peels as dietary fiber sources with the advantage of shorter processing times and lower temperatures than conventional technologies used to treat food fibers.  相似文献   

20.
Oleoresin sage (Salvia officinalis) (SAG) (200–1500 mg kg?1), ascorbyl palmitate (AP) (100–300 mg kg?1) and TBHQ (200 mg kg?1) were assessed for delaying the thermo‐oxidation in sunflower oil (SO) during 18 h of frying (180 °C). Electronic nose compared the global aroma fingerprints of potato chips fried in oils. The chemical rancidity indices viz., fatty acids, total polar compounds (TPC), altered triglycerides (dimers, polymers, oxidised monomers, diglycerides), free fatty acids, conjugated dienes and induction periods were monitored along with physical indices viz., viscosity and colour. SOSAG+AP (1309.62 + 270.71 mg kg?1) outperformed SOTBHQ by preserving polyunsaturated fatty acids (60.48% vs. 56.23%), retarding TPCs (28.16% vs. 29.91%), triglyceride dimers (90.24 vs. 95.82 mg g?1) and polymers (25.40 vs. 26.98 mg g?1) concomitantly extending the oil disposal time (basis 25% TPC) (15.9 vs. 14.7 h). The postfrying viscosity, colour values and global aroma fingerprints of fried chips indicate a close match between SOSAG+AP and SOTBHQ.  相似文献   

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