Evaluation the efficacy of extrusion pretreatment via enzymatic digestibility and simultaneous saccharification & fermentation with rapeseed straw

The efficacy of extrusion pretreatment was evaluated by enzymatic hydrolysis and simultaneous saccharification and fermentation (SSF) with straw of rapeseed, Brassica napus, an agricultural residue. An acceptable pretreatment result was obtained at a barrel temperature of 165 °C, acid concentration of 20 g L⁻¹, liquid feeding rate of 13.4 cm³ min⁻¹, solid feeding rate of 1.0 g min⁻¹, screw rotation speed of 6.3 rad s⁻¹, and residence time of 10.2 min, with a yield of xmg, representing the sum of the corresponding hydrolyzed sugars; xylose, mannose and galactose, of 794.3 g kg⁻¹ and a glucose release of 21.0 g kg⁻¹. These were calculated to be 963.0 g kg⁻¹ and 910.3 g kg⁻¹ based on cellulose and hemicellulose recoveries,respectively. The highest enzymatic digestibility of 781.0 g kg⁻¹was higher than that obtained from the batch pretreatment with dilute acid by 1.4-fold. The SSF process afforded an ethanol concentration of 16.0 g L⁻¹, corresponding to an ethanol yield of 790 g kg⁻¹ based on the total available cellulose in the pretreated rapeseed straw.     

ABE fermentation from enzymatic hydrolysate of NaOH-pretreated corncobs

Acetone butanol ethanol (ABE) was produced from enzymatic-hydrolyzed corncobs by Clostridium saccharobutylicum DSM 13864. Pretreatment of corncobs was carried out with 0.5 mol L⁻¹ NaOH followed by enzymatic hydrolysis. The yield of total reducing sugars was 917 g kg⁻¹ pretreated (de-lignified) and washed corncobs. The hydrolysate was used without sediments removal for ABE fermentation. A solvent production of 19.44 g L⁻¹ with 12.27 g L⁻¹ butanol was obtained from 55.22 g L⁻¹ sugars, resulting in an ABE yield of 350 g kg⁻¹ and a production rate of 0.54 g L⁻¹ h⁻¹. A control experiment using 55.3 g L⁻¹ mixed sugars resulted in an ABE production of 16.81 g L⁻¹ with 10.26 g L⁻¹ butanol, corresponding to an ABE yield of 300 g kg⁻¹ and a production rate of 0.47 g L⁻¹ h⁻¹, indicating that the enzymatic hydrolysates may contain stimulating compounds that can improve the ABE fermentation.     

Sickle bush (Dichrostachys cinerea L.) field performance and physical–chemical property assessment for energy purposes

The sickle bush (Dichrostachys cinerea (L.) Wight & Arn.) comprises a woody legume shrub which is widely distributed throughout of the tropical areas of Africa, Asia and Oceania, being found as well in Cuba where it represents a difficult to control invasive plant. It holds great silvopasture and energy crop potentials. In southwestern Spain a two year field trial was conducted contemplating also another six hardwood taxa commonly used as energy crops. The sickle bush above ground dry biomass fraction was 60.4%; sickle bush displayed a high transpiration rate during hot days (3.02 kg m⁻² d⁻¹ to 6.82 kg m⁻² d⁻¹); cold winter temperatures (<−2 °C) together with hot and dry summer air (<20% relative humidity) committed survival and growth. The physical-chemical wood properties and the pellets thereof derived were analyzed and compared to those of the other energy crop taxa. The within other woody species normal chemical composition range coupled to a high wood density and energetic use properties (19.2 MJ kg⁻¹ higher heating value, 29 g kg⁻¹ ash content) all allow for an industrial use. Pellets evidenced also good physical and mechanical properties (690 kg m⁻³ bulk density, 42 g kg⁻¹ moisture content). However, the mechanical durability (93.9%) was slightly less than that required by the non-industrial use standards, therefore further improvements should be studied. All of the above could encourage scrubland cuts in Cuba as a mechanical control method, in addition to the expansion of plantations within of their tropical climate based natural habitats.     

Use of treated effluent water in ethanol production from cellulose

The bioethanol industry exerts a significant demand on water supplies. Current water consumption rate in corn dry grind ethanol plants is (11–15) dm³ m⁻³ of ethanol produced and (23–38) dm³ m⁻³ for cellulosic ethanol plants. The main goal of this study was to examine the feasibility of use of treated wastewater effluent in place of potable freshwater for cellulosic ethanol production. The effects of using two different types of filtered treated effluent; Bloomington- Normal, IL (Residential type) and Decatur, IL (Industrial/Residential Mix type); on the rate of fermentation and final ethanol yield from a pure cellulosic substrate were evaluated. Characterization analysis of both effluent water samples indicated low concentration of toxic elements. Final ethanol concentrations obtained with Bloomington- Normal and Decatur effluent and with a control treatment using de-ionized water were similar, resulting in 360 g kg⁻¹ (0.36 g g⁻¹), 370 g kg⁻¹ (0.37 g g⁻¹) and 360 g kg⁻¹ (0.36 g g⁻¹), respectively. These findings suggest that with proper characterization studies and under appropriate conditions, the use of treated effluent water in cellulosic ethanol production is feasible.     

Drying of firewood - the effect of harvesting time, tree species and shelter of stacked wood

Firewood represents a renewable source of energy and is the main source of energy for about half the World’s population. When burning firewood in domestic stoves, combustion and thus energy efficiency is dependent on the moisture content of the wood. In Denmark, it is generally recommended that moisture content should be no more than 180 g kg⁻¹ total weight. This study aims to assess the effect of species, harvesting time and shelter on the drying of stacked firewood. After felling, the moisture content declined to a relative stable level for all species. The rate of drying depended on the felling time, tree species, and the presence of shelter. The lower asymptotic moisture content depended mainly on the presence of shelter and averaged 188 g kg⁻¹ total weight for frames left in the open and 154 g kg⁻¹ total weight for frames covered by a shelter. It is concluded that Norway spruce felled during the early summer may obtain an acceptable moisture content at the onset of the heating season. Deciduous trees should be felled during the winter or early spring and stored under shelter to be suitable for burning before the heating season. Shelter was found to be of great importance to maintain an acceptable moisture content of firewood in storage during winter.     

Production of xylose and glucose from rapeseed straw in subcritical water – Use of Doehlert design for optimizing the reaction conditions

The hydrothermal depolymerization of the hemicellulosic and cellulosic fractions of rapeseed straw was carried out in a batch reactor in subcritical water. The experimental design methodology (Doehlert matrix) was used to model the hydrothermal process and to optimize operational parameters reaction temperature and holding time for highest yields of saccharides: xylose and glucose, obtained in experimental series 1 and 2, respectively. In good agreement with experimental results, the model predicts an optimal yield of xylose (60.4 g kg⁻¹ ± 2.1 g kg⁻¹) at a temperature and a holding time of about 203 °C and 13 min. The optimal yield of glucose (180.7 g kg⁻¹ ± 2.8 g kg⁻¹) required higher temperatures but shorter times and was obtained at a temperature 255 °C and a holding time of 9 min. A further increase of both reaction parameters would lead to the undesirable transformation of the saccharides: their dehydration, retro-aldol condensation, isomerization and tautomerization. The obtained results confirm that the hydrothermal treatment of rapeseed straw in optimal reaction conditions might be an alternative route for the production of xylose and glucose.     

Microbial changes during the on-farm storage of canola (oilseed rape) straw bales and pellets

The effect of on-farm storage on microbial growth on baled and pelletised Brassica napus (oilseed rape/canola) straw was investigated. Canola straw collected in 2008 and 2009 was stored baled in an open shed for 3, 4, 7, 10 and 20 months in 2008 and for 1 and 3 months in 2009. Pellets were produced from straw stored for 3, 7, and 10 months in 2008 and straw stored for 3 months in 2009, and stored for up to 48 weeks.The moisture content (MC), water activity (a_w), bacterial and fungal colony-forming units (CFU), and carbon-to-nitrogen ratio (C:N) of canola straw bales and pellets were measured during storage. In addition, temporal environmental conditions (ambient temperature and relative humidity) and bale temperature were monitored. The moisture content showed a tendency to stabilise during storage, with an equilibrium moisture content of approximately 155 g kg⁻¹ total weight for straw bales and 110 g kg⁻¹ total weight for straw pellets. Consequently, the water activity of canola straw bales remained below 0.8 and that of pellets below 0.66 during storage, providing an explanation for relatively low microbial growth. The number of bacterial and fungal CFU present in the straw bales and pellets followed the trend of ambient relative humidity and no correlation was found with the C:N ratio of the biomass. Canola straw pellets were considered a superior combustion fuel to straw bales due to lower moisture content and less microbial deterioration during storage.     

Energy sorghum hybrids: Functional dynamics of high nitrogen use efficiency

The nitrogen use efficiency (NUE) of high biomass energy sorghum hybrid plants increased during 180 days of growth to a maximum of 370 g DW g⁻¹ N⁻¹. Shoot N uptake was biphasic and continued for 120 days. Leaf N accumulation was rapid until day 60. Specific leaf nitrogen (SLN) varied from 0.9 to 1.7 g N m⁻² green leaf area, a typical range for C4 grass canopies. Stem N increased to a maximum at day 120. NUE increased during development in parallel with increasing stem to leaf biomass ratio and as stems decreased from 0.7% to 0.2% N. At the end of the season, green leaves were ∼1% N, represented 17% of total shoot biomass and accounted for 50% of N present in shoots (above ground biomass) while stems were ∼0.2% N, comprised 83% of shoot biomass and accounted for 50% of shoot N. High NUE was due in part to N-remobilization from lower leaves and stem nodes/internodes to upper portions of the canopy. Up to 70% of dry weight and 90% of N was remobilized during senescence of lower leaves and 70% of N was remobilized from lower stem nodes/internodes. The NUE of energy sorghum was similar to Saccharum officinarum and Miscanthus x giganteus, and higher than grain Sorghum bicolor, Zea mays, and Panicum virgatum. High NUE of energy S. bicolor is due to long duration of vegetative growth, high stem to leaf biomass ratio, and very efficient N-remobilization from lower leaves and stem internodes during development.     

High dry matter whole-plant corn as a biomass feedstock

This research investigated the harvest, ambient pre-treatment, and storage of whole-plant corn as an alternative to conventional systems where corn grain and stover are fractionated at harvest. Harvesting the whole-plant, both grain and most of the above ground stover, after physiological maturity can reduce the intense logistics challenges typically associated with corn harvest and expand the harvest window. To determine the feasibility of the proposed system, corn was harvested at 350–840 g kg⁻¹ whole-plant dry matter (DM) using a forage harvester and then ensiled in pilot-scale silos. Ambient pretreatment during storage was investigated using both dilute acid and lime. Both pretreated and control whole-plant silages were very well conserved during anaerobic storage with DM losses generally less than 40 g kg⁻¹. Hydrodynamic separation of the grain and stover fractions after storage was found to be more effective at fractionating starch and fiber than conventional dry grain harvest, and both fractions had desirable composition. The effects of pretreatment on the silage were very pronounced at 30 and 100 g (kg DM)⁻¹ sulfuric acid loading with less than 100 g (kg DM)⁻¹ of the hemicellulose still bound in the cell wall at DM contents greater than 500 g kg⁻¹. The whole-plant harvest and storage system was shown to be a viable alternative to conventional corn grain and stover systems for producing feedstocks for biochemical conversion.     

Response surface design to study the influence of inoculum,particle size and inoculum to substrate ratio on the methane production from Ulex sp.

Ulex europaeus is one of the world worst invaders vegetal species and its suitability for biogas production is significant. The effect of three factors affecting the Biochemical Methane Potential (BMP, expressed as volume of CH₄ per mass of volatile solids of waste) and the biodegradability rate (k, expressed in volume of CH₄ per mass of VS and time) of U. europaeus was assessed by a Central Composite Face Centred Design. The BMP varied from 153 L kg⁻¹ to 308 L kg⁻¹. Inoculum to substrate ratio (ISR) and the type of inoculum had high influence on the final results. k varied from 14 L kg⁻¹ d⁻¹ to 49 L kg⁻¹ d⁻¹. The conditions that simultaneously maximized the BMP and k were an inoculum consisting in 55% (v) of granular sludge and 45 % (v) of suspended sludge from a sludge digester, an ISR of 4 g g⁻¹, and a particle size of 1.9 mm. Considering the average biomass production in shrub land areas, the potential energy production from U. europaeus is estimated in (36.9 ± 19.3) GJ ha⁻¹ yr⁻¹. For example, in Europe, a maximum energy supply of 7 EJ yr⁻¹ could be achieved from potentially harvestable shrub land areas.     

Catalytic conversion of Helianthus tuberosus L. to sugars, 5-hydroxymethylfurfural and levulinic acid using hydrothermal reaction

In this study, the efficient conversion of Helianthus tuberosus L. (Jerusalem artichoke) to sugar, 5-hydroxymethylfurfural (5-HMF), and levulinic acid (LA) were investigated using a Brønsted acid-catalyzed hydrothermal reaction. From this approach, the optimal amounts and reaction conditions for total reducing sugar (TRS), 5-HMF, and LA were as follows: 444 g kg⁻¹ of TRS yield (150.4 °C, 14.6 kg m⁻³ sulfuric acid, and 9.8 min), 193 g kg⁻¹ of 5-HMF yield (166.0 °C, 4.6 kg m⁻³, 30.8 min), and 323 g kg⁻¹ of LA yield (185 °C, 30.8 kg m⁻³, 33.7 min). In terms of combined severity, the TRS and 5-HMF concentrations decreased linearly with increasing combined severity, whereas, the LA concentration was increased at a high combined severity factor. The H. tuberosus L. may be useful as a significant feedstock for the production of platform chemicals such as 5-HMF and LA.     

Biomethane Production from co-fermentation of agricultural wastes

Biomethane (CH₄) was recovered from co-digestion process of waste glycerol and banana wastes. The wastes used contain waste glycerol with varying concentrations from 7.5 to 90 g L⁻¹ and banana peel in the range 2.5–10 %w·v⁻¹. The co-substrate mixture ratio was implemented in 0.5 L batch reactor operated at 37 °C and pH 7 for 120 h. The composition of biogas gas and liquid samples (COD, VFA, pH, alkalinity) were analyzed every 12 and 24 h, respectively. The optimum condition to produce CH₄ was found at 7.5 g L⁻¹ waste glycerol mixed with 7.5% banana peel. The highest CH₄ yield and CH₄ production potential were 0.281 m³ kg⁻¹ COD and 652 mL, respectively.     

Fuel ethanol production from sweet sorghum bagasse using microwave irradiation

Sweet sorghum is a hardy crop that can be grown on marginal land and can provide both food and energy in an integrated food and energy system. Lignocellulose rich sweet sorghum bagasse (solid left over after starch and juice extraction) can be converted to bioethanol using a variety of technologies. The largest barrier to commercial production of fuel ethanol from lignocellulosic material remains the high processing costs associated with enzymatic hydrolysis and the use of acids and bases in the pretreatment step. In this paper, sweet sorghum bagasse was pretreated and hydrolysed in a single step using microwave irradiation. A total sugar yield of 820 g kg⁻¹ was obtained in a 50 g kg⁻¹ sulphuric acid solution in water, with a power input of 43.2 kJ g⁻¹ of dry biomass (i.e. 20 min at 180 W power setting). An ethanol yield based on total sugar of 480 g kg⁻¹ was obtained after 24 h of fermentation using a mixed culture of organisms. These results show the potential for producing as much as 0.252 m³ tonne⁻¹ or 33 m³ ha⁻¹ ethanol using only the lignocellulose part of the stalks, which is high enough to make the process economically attractive.     

Lignocellulosic ethanol production employing immobilized Saccharomyces cerevisiae in packed bed reactor

Most of ethanol production processes are limited by lower ethanol production rate and recyclability problem of ethanologenic organism. In the present study, immobilized co-fermenting Saccharomyces cerevisiae GSE1618 was employed for ethanol fermentation using rice straw enzymatic hydrolysate in a packed bed reactor (PBR). The immobilization of S. cerevisiae was performed by entrapment in Ca-alginate for optimization of ethanol production by varying alginic acid concentration, bead size, glucose concentration, temperature and hardening time. Remarkably, extra hardened beads (EHB) immobilized with S. cerevisiae could be used up to repeated 40 fermentation batches. In continuous PBR, maximum 81.82 g L⁻¹ ethanol was obtained with 29.95 g L⁻¹ h⁻¹ productivity with initial glucose concentration of 180 g L⁻¹ in feed at dilution rate of 0.37 h⁻¹. However, maximum ethanol concentration of 40.33 g L⁻¹ (99% yield) with 24.61 g L⁻¹ h⁻¹ productivity was attained at 0.61 h⁻¹ dilution rate in fermentation of un-detoxified rice straw enzymatic hydrolysate (REH). At commercial scale, EHB has great potential for continuous ethanol production with high productivity using lignocellulosic hydrolysate in PBR.     

Effect of temperature on the fast pyrolysis of organic-acid leached pinewood; the potential of low temperature pyrolysis

In this paper, we have evaluated the potential of organic acid (mixture of acetic, formic and propionic acid) leaching of biomass and subsequent fast pyrolysis to increase the organic oil, sugars and phenols yield by varying the fluidized bed temperature between 360 °C and 580 °C (360 °C, 430 °C, 480 °C, 530 °C, and 580 °C). The pyrolysis of acid leached pinewood resulted in more organic oil and less water and residue compared to untreated pinewood over the whole temperature range. Below 500 °C the difference was most profound; for acid leached pinewood at 360 °C the organic oil was already 650 g kg⁻¹ pine with a sugar yield of 230 g kg⁻¹ pine. At this low pyrolysis temperature no bed agglomeration was observed for acid leached pine whereas at the higher temperatures tested agglomerates were found, which were identified to be clusters of fluidization sand glued together by sticky pyrolysis products (melt). Low reactor temperatures also favored the production of monomeric phenols, though their absolute yields remained low for both untreated and leached pine (maximum: 23 g kg⁻¹ pine, 80 g kg⁻¹ lignin). GPC, GC/MS and UV-fluorescence spectroscopy showed that acid leaching did not influence significantly the yield and molecular size of the aromatic fraction in the produced pyrolysis oils. Back impregnation of the removed AAEMs into leached biomass revealed that the effects of the applied acid leaching, both with respect to the product yields and bed agglomeration, can be mainly assigned to the removal of AAEMs.     

Assessment of triacylglycerol content in Chlorella vulgaris cultivated in a two-stage process

Chlorella vulgaris cultivation in two-stage process was applied to increase the lipid productivity without compromising the biomass productivity. At the first stage, microalgae was cultivated under nutrient sufficient conditions to obtain a maximized cell density; at the second stage, nitrate conditions are changed to trigger the accumulation of TAG. During first stage, the maximum biomass productivity (32 mg L⁻¹ d⁻¹) was observed after 13 days under nutrient sufficient conditions with 1.21 g L⁻¹ NaNO₃ and 0.00449 g L⁻¹ K₂HPO₄. Maximum lipid content (25.4% DW), lipid productivity (7.5 mg L⁻¹ d⁻¹) and TAG content (41.3% in total lipids) were favored by the nitrogen starvation conditions for more 4 days, at the second stage. Oil extracted at the second stage contained lower percentage of PUFAs being more suitable for the biodiesel production when compared with the oil extracted at the first stage. This two-stage phototrophic process is promising to provide a more efficient way for on a large-scale production of algal biomass and biodiesel production.     

Production and detailed characterization of bio-oil from fast pyrolysis of palm kernel shell

Bio-oil has been produced from palm kernel shell in a fluidized bed reactor. The process conditions were optimized and the detailed characteristics of bio-oil were carried out. The higher feeding rate and higher gas flow rate attributed to higher bio-oil yield. The maximum mass fraction of biomass (57%) converted to bio-oil at 550 °C when 2 L min⁻¹ of gas and 10 g min⁻¹ of biomass were fed. The bio-oil produced up to 500 °C existed in two distinct phases, while it formed one homogeneous phase when it was produced above 500 °C. The higher heating value of bio-oil produced at 550 °C was found to be 23.48 MJ kg⁻¹. As GC–MS data shows, the area ratio of phenol is the maximum among the area ratio of identified compounds in 550 °C bio-oil. The UV–Fluorescence absorption, which is the indication of aromatic content, is also the highest in 550 °C bio-oil.     

Hydrothermal conversion of water lettuce biomass at 473 or 523 K

The high moisture content of an aquatic biomass was used advantageously in a hydrothermal process. Reducing sugars, amino acids, proteins, and crude oil were extracted from water lettuce (Pistia stratiotes L.) using subcritically heated water. The highest yields of reducing sugars and amino acids were obtained after treatment at 473 K for 30 min (23.70 ± 0.52 g kg⁻¹ and 4.35 ± 0.09 g kg⁻¹ dry mass respectively), while protein was obtained at 3.60 ± 0.04 g kg⁻¹ feedstock after treatment at 523 K for 60 min. The greatest solubilization occurred at 523 K after 60 min. The solid residues could be applied as fertilizers as hemicellulose and cellulose were hydrothermally converted to humus. The crude oil components that were extracted from the liquid residues differed markedly between the two treatment temperatures. The conversion of furan compounds to cyclopentenone and its derivatives only occurred at the higher reaction temperature and was increased by a longer reaction time.     

Facile sonochemical synthesis of strontium phosphate based materials for potential application in supercapattery devices

Among hybrid energy storage devices, supercapattery gained profound research interest due to its ability to give high energy density while maintaining the power density and cyclic stability. Herein, novel low-cost strontium based materials are synthesized by controlled sonochemical method and subsequently calcined at various temperatures. The multiple phases of the material synergistically contributed in the electrochemical charge storage process and give high specific capacity of 220 C g⁻¹ (as-prepared material) and 213 C g⁻¹ (calcined at 200 °C) at 0.5 A g⁻¹. A thorough electrochemical performance of optimized material is investigated as an electrode in asymmetric device. The supercapattery (SP2//AC) exhibits a specific capacity of 103.4 C g⁻¹ at 0.5 A g⁻¹ in the voltage range of 0–1.7 V. Furthermore, supercapattery offers a considerably high specific energy of 24.4 Wh kg⁻¹ at a specific power of 425 W kg⁻¹ and an excellent specific power of 1870 W kg⁻¹ by maintaining specific energy at 14.5 Wh kg⁻¹. In addition, the device retained its specific capacity to 90% after 3000 charging/discharging cycles at 1 A g⁻¹. Strontium based materials could be proposed as an appropriate electrode material for energy storage systems.     

Efficient ethanol production from inulin by two-stage aerate strategy

A major concern for ethanol production from inulin-containing materials, is the higher unconverted sugar, which increases the cost of ethanol production and wastewater treatment. Some key factors, such as inulinase, biomass or aeration rates, were studied to solve the problems in the process of ethanol fermentation from inulin. It was showed that more inulinase and increasing inoculum size can shorten the fermentation time, but could not reduce residual sugars. Two-stage aerate strategy was developed to utilize the remained sugars: keep the aeration at 5 h⁻¹ at the first 12 h, and drop it to 1.2 h⁻¹. Under this condition, contradiction between fermentation time and high ethanol yield was solved (60 h and 0.43 g g⁻¹), and the final residual sugar concentration decreased to about 10 g L⁻¹ with 98 g L⁻¹ ethanol. The ethanol productivity was up to 1.63 g L⁻¹ h⁻¹, which is the highest productivity of ethanol fermentations from inulin-containing materials.