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A novel and convenient method based on high-performance liquid chromatography coupled with chemiluminescence (HPLC) for the determination of propyl gallate in edible oil is reported. The detection was based on the inhibition of chemiluminescence between luminol and hydrogen peroxide in alkaline solution. The analysis was performed using a C18 column with isocratic 60:40 methanol/water. Under the optimum conditions, the concentration of propyl gallate was linear from 9?×?10?6?mol?L?1 to 1?×?10?4?mol?L?1 with a detection limit of 2?×?10?7?mol?L?1. The method is simple, sensitive, and inexpensive and was used for the determination of propyl gallate in edible oil. 相似文献
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Baicalin was determined in cosmetics and toothpaste by high-performance liquid chromatography. The samples were extracted with ultrasound in 75% ethanol for 20 min, purified by anion exchange solid-phase extraction cartridge, and the components were separated on a C18 column. The limits of detection and quantification were 3.0 mg kg?1 and 10.0 mg kg?1. The recovery was from 81.3–101.6%. A linear relationship was present from 1.00–100 µg · mL?1 of baicalin. The protocol was simple, sensitive, selective, and used to determine baicalin in personal care products. Baicalin was not present in materials contrary to the labeled contents. 相似文献
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A liquid chromatographic method is described for the quantification of prednisolone, benzimidazoles, and preservatives using a C18 analytical column as stationary phase. The mobile phase was 30:70 methanol:pH 2.5 phosphate buffer at a flow rate of 1.0 mL min?1 with absorbance detection at 235 nm. The method was linear for concentrations ranged from 40–10,000 ng mL?1. Low values of coefficient of variance were obtained when samples were analyzed as replicates. Excellent recovery values were recorded in commercial products and fortified samples. International Conference of Harmonization protocols were employed to perform comprehensive method validation. The reported method has applications for pharmaceutical and serum samples. 相似文献
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A novel and rapid method was validated for the determination of phenolics in plants by high-performance liquid chromatography coupled to photodiode array detection. The optimized method was employed for the quantification of phenolic acids and rutin present in Heliotropium thermophilum. Eight samples collected from two locations were analyzed. Additionally, temperature dependent changes in the phenolic composition of H. thermophilum samples was evaluated. A series of standards were used for identification that included gallic acid, protocatechuic acid, 4-hydroxybenzoic acid, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, rutin, ferulic acid, benzoic acid, 2-hydroxycinnamic acid, abscisic acid, and trans-cinnamic acid. Chromatographic separation was performed on C8 column at 40°C with gradient elution. A phosphate buffer (20 mM, pH: 2.5) and acetonitrile mixture was used as the mobile phase with detection at 280 nm wavelength and a flow rate of 1.5 mL/min. The developed method was shown to be sensitive, accurate, and reproducible for the determination of phenolic acids and rutin in plants. 相似文献
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Food contamination is a major global issue and the challenges are increasing. Alternative methodology using lipids in detecting contaminants and determining food ingredients is needed to supplement or replace current methodology. The core–shell column is considered to be a breakthrough for high-performance liquid chromatography. In this study, lipid profiling was performed using a C18 core–shell column to separate and identify triacylglycerols in oil and food. The chromatographic profiles were compared at various flow rates to determine their efficiency. The evaluation was done based on chromatographic resolution, resolving power, and separation efficiency. In addition, the height equivalent to a theoretical plate of the column was determined and a comparison was made at the same linear velocity along with the peak capacity. The most efficient flow rate was 400?µL/min, and by applying the methodology, triacylglycerols from edible oil and food were extensively profiled. The results show that triacylglycerol profiles may be utilized as a biomarker for food quality. This alternative methodology provided a comprehensive profile of triacylglycerols in oil and food that allowed the identification of unique triacylglycerol biomarkers for practical analysis. 相似文献
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An alternative, effective, and solvent-free microextraction method has been developed using weighing paper as the adsorbent for five protoberberine alkaloids from the methanolic extract of Coptidis Rhizoma (C. Rhizoma). Several variables influencing extraction efficiency were optimized. 1.0?×?1.0?cm glassine weighing paper was employed for the isolation of the analytes followed by high-performance liquid chromatography. Under the optimum conditions, the limits of detection were between 0.2 and 0.4?ng/mL with enrichment factors from 41–47. The calibration curves were linear across the concentration ranges examined with correlation coefficients exceeding 0.992. All relative standard deviations were less than 10.0%. The method was employed for the determination of the analytes in C. Rhizoma. Berberine, palmatine, coptisine, epiberberine, and jatrorrhizine were present at concentrations between 9.62?±?0.87?mg/g and 0.48?±?0.05?mg/g. 相似文献
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高效液相色谱法测定甘蓝、青菜和番茄中虫酰肼、甲氧虫酰肼和呋喃虫酰肼残留 总被引:4,自引:0,他引:4
建立了同时测定蔬菜(甘蓝、青菜和番茄)中虫酰肼、甲氧虫酰肼和呋喃虫酰肼(福先)农药残留的高效液相色谱法。以酸化甲醇高速匀浆提取,正己烷去除脂类杂质,二氮甲烷反革取去除亲水性杂质,再经氟罗里硅土柱层析净化,效果显著。采用甲醇-水(75+25,V/V)为流动相,利用Agilent HC—C18柱和紫外检测器(检测波长:230nm)对待测组份进行了分离和测定。实验证明,添加浓度在0.05mg/kg,0.2mg/kg和1mg/kg时,蔬菜(甘蓝、青菜和番茄)中虫酰肼、甲氧虫酰肼和呋喃虫酰肼平均添加回收率在76.9%~105%之间,相对标准偏差(RSD,n=5)小于10%,虫酰肼、甲氧虫酰肼和呋喃虫酰肼在样品中的最低检出浓度为0.05mg/kg。 相似文献
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在ASTM6379标准方法的基础上,建立火箭煤油中芳烃的液相色谱-紫外检测方法。该方法采用氨基柱串联氰基柱,以正己烷为流动相,流速1.0mL/min,柱温28℃。单环芳烃以邻二甲苯计在8~80mg/L(r2=0.9999)范围内线形关系良好,定量限0.008mg/L,在已知浓度的样品溶液中分别加入高、中、低3种不同浓度的标准品溶液,回收率85.4%~114.1%,相对标准偏差小于5%。该方法简便易行、准确可靠。 相似文献
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采用高效液相色谱-电喷雾串联质谱仪(LC -ESI-MS-MS),建立猪肉中林可霉素、竹桃霉素、替米考星、泰乐菌素、克林霉素、吉他霉素、交沙霉素等7种大环内酯类药物残留的检测方法,试验中猪肉中大环内酯类抗生素的残留用乙腈提取,以罗红霉素作为内标,提取液用正己烷去除脂肪后浓缩,再用磷酸盐溶液溶解后,经HLB固相萃取柱净化,甲醇洗脱,洗脱液浓缩定容至1mL ,供液相色谱-串联质谱法测定,内标法定量。本方法采用10μg/kg ,20μg/kg ,100μg/kg ,3个添加浓度,每个浓度6个平行样品,上述7种大环内酯类药物残留的回收率在75%~100%,相对偏差在1.51%~5.48%。 相似文献
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固相萃取-高效液相色谱法测定水中甲萘威 总被引:1,自引:0,他引:1
采用固相萃取,高效液相色谱法对水中甲萘威进行检测,通过实验研究,该法对水中甲萘威的最低检测限为0.001mg/L,相对标准偏差(RSD,n=5)≤2.5%,加标回收率为98.0%~98.5%,固相萃取效果优于液液萃取。该方法适合于地表水及饮用水中甲萘威的检测。 相似文献
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建立了高效液相色谱-电感耦合等离子体质谱 (HPLC-ICP-MS) 联用技术测定玩具中痕量可迁移有机锡的方法。采用Waters Acquity UPLC HSS T3分析柱,在流动相为V(乙腈)∶V(水)∶V (乙酸)=65∶23∶12的混合溶液中加入0.1%三乙胺作为离子对试剂,在pH 3.0条件下,可以很好地分离二丁基锡(DBT)、三苯基锡(TPhT)、三丁基锡(TBT)和二辛基锡(DOT)。此方法简便、快速、灵敏度高,在50 μL进样量下,DBT、TPhT、TBT及DOT的检出限分别为0.08、0.24、0.14和0.65 μg/L;在5、50 μg/L两个加标水平下,回收率分别为80.7%~96.3%和83.5%~100.4%,方法精密度优于4.7%,可以满足测定要求。应用该方法测定了玩具可接触部分材料,包括织物、木料、塑料、涂层中的可迁移有机锡,分析结果令人满意。 相似文献
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清肝散结汤(QGSJ)是由14味不同中药组成的复方制剂,基于长期的实验观察和临床经验以不同组合成方用于肝癌的治疗。本研究建立了一种基于加速溶剂萃取(ASE)结合高效液相色谱-电喷雾飞行时间质谱(HPLC-ESI-TOF MS)的方法同时测定清肝散结汤中29种化学成分。使用70%乙醇作为提取溶剂,提取温度100 ℃,静态萃取时间5 min,提取2次,以上条件采用正交设计和主成分分析进行优化。HPLC分离采用Kromasil C18色谱柱,梯度洗脱。TOF MS在负离子模式下检测,质量扫描范围m/z 100~1100。结果表明:清肝散结汤中29种成分具有良好的线性关系(r>0.994)和日内、日间精密度(RSD<5%);提取回收率在96.5%~104.5%之间。该方法快速、可靠,适用于复方中药的定量评价。 相似文献
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利用反相高效液相色谱法同时测定苹果、梨、橙子中草酸、酒石酸、乙酸、乳酸4种有机酸的含量.采用Waters2695 HPLC-PDA色谱系统,Waters Xbridge C18(4.6×250mm,5 μm)色谱柱,乙腈和0.01 mol/L磷酸二氢钠溶液(pH=2.6)作为流动相,流速1.00mL/min,柱温30℃,紫外检测波长214nm的色谱条件下,能够在10分钟内较好地定量分析水果中4种有机酸的含量,本方法回收率为92.17%-103.19%,相对标准偏差为0.23-0.65,各有机酸标准曲线相关系数均在0.9997以上.本方法准确、高效,灵敏度高,适用于水果中有机酸的检测. 相似文献
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固相萃取-高效液相色谱法测定水中12种酚类化合物 总被引:6,自引:0,他引:6
采用固相萃取,高效液相色谱分离,紫外分光光度法对水中12种酚类物质进行检测。通过实验研究,该法对12种酚的最低检测限为0.17~0.56μg/L,相对标准偏差(RSD,n=7)小于等于6.5%,加标回收率为86.67%~106.33%。该方法适合于地表水及饮用水中酚类化合物的检测。 相似文献